A new look. New plasmids. Streamlined ordering.
As of July 17th, 2019 the new Gateway vector website has launched. We are still providing the same Gateway plasmids to the research community but with a more up-to-date look and streamlined ordering process. We are also making available a number of Golden Gate vectors derived from the GreenGate system. Please bear with us as we continue to update the collection and associated information. If you see a problem, or need additional information, please contact us.
Please note that all users of the previous website will need to create new accounts to place orders on this system. We were unable to carry over the previous user accounts.
Gateway and Golden Gate vectors for plant transformation
Gateway technology by Invitrogen provides a quick method for cloning a DNA fragment into multiple expression vectors. At VIB-Ugent Center for Plant Systems Biology, we have constructed over 200 versatile vectors for gene functional analysis in plants and other species. The plasmid accessions offered by PSB comprise Gateway entry clones, intermediary plasmides and destination vectors for transgene construction. Additionally, a growing collection of Golden Gate vectors has been made available as well. This collection contains a wide range of entry modules (promoters, tags and other building blocks) and destination vectors. All plasmids are documented with a graphical map and the corresponding genbank (.gb) file.
Introduction to our plant vectors
Most of the binary constructs we built for Agrobacterium plant transformation are based on the pPZP200 backbone (see below). These constructs carry one of the three most frequently used plant marker genes, selectable with kanamycin, hygromycin or phosphinotricin (Basta). These genes were cloned toward the left border of the T-DNA.
Our constructs also include E. coli high copy number plasmids, useful for transient gene expression in plant cells. When relevant, Gateway vectors conform with the rfA conversion rules.
Gateway plasmid accessions built by other groups are available from our stocks.
This distribution of Gateway vectors is supported by the group of Plant Genome Editing from the VIB-UGent Center for Plant Systems Biology.