LOCUS       pGG-E-AtU6-26-BbsI-ccdB-BbsI-F        3801 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...k2-8129d0b
VERSION     urn.local...k2-8129d0b
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="E"
     promoter        14..401
                     /modified_by="thjac"
                     /label="AtU6-26"
     UTR             410..728
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /annotation_group="ccdB 5'UTR: 441 -> 759"
                     /label="ccdB 5'UTR"
     CDS             729..1034
                     /label="ccdB"
     scaffold        1044..1126
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="thjac"
                     /label="Scaffold"
     GG              complement(1127..1137)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:83-dgycryy"">F</a>"
                     /label="F"
     primer          complement(1157..1177)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1657..1761
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1762..2622
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2793..3381
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3745..3766
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cctctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
       61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
      121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
      181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
      241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
      301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
      361 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggggtcttca agccagataa
      421 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
      481 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
      541 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
      601 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
      661 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
      721 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
      781 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
      841 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
      901 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg
      961 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1021 tctggggaat ataaggaaga caagttttag agctagaaat agcaagttaa aataaggcta
     1081 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttacta tgagaccgaa
     1141 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     1201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1321 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1381 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1441 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1501 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1561 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1621 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1741 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1801 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1861 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1921 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1981 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2041 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2101 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2161 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2221 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2281 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2341 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2401 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2461 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     2521 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2581 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2641 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2701 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3481 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3541 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3601 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3661 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3721 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     3781 gacactatag aagtgaagct t
//
