LOCUS       pGG-A-AtU6-26-BbsI-BbsI-B        3177 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...tm-7wm1biz
VERSION     urn.local...tm-7wm1biz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        14..401
                     /modified_by="User"
                     /label="AtU6-26"
     Scaffold        420..502
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:.:7o-dgycryx"">SpCas9 Scaffold</a>)"
     GG              complement(503..513)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(533..553)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1033..1137
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1138..1998
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2169..2757
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3121..3142
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tctctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
       61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
      121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
      181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
      241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
      301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
      361 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggggtcttcg agaagacctg
      421 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      481 gcaccgagtc ggtgcttttt ttaacatgag accgaattct cgccctatag tgagtcgtat
      541 tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      601 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      661 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      721 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
      781 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      841 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      901 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
      961 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1021 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1081 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1141 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1201 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1261 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1321 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1381 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1441 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1501 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1561 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1621 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1681 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1741 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1801 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga
     1861 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1921 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1981 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2041 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2281 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2881 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2941 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3001 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     3061 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     3121 gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
