LOCUS       pGG-C-Cas9PTA-D         6828 bp    DNA     circular     14-MAR-2023
DEFINITION  .
ACCESSION   urn.local...fj-citr5zk
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11464398|                                                 
            VNTDBDATE|-11464411|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-C-Cas9PTA-D|                                           
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            "Label 3") 4                         (CShapeMapEntry 0 "Shape 33" 1
            "Label 21") 29                       (CShapeMapEntry 0 "Shape 16" 1
            "Label 1") 27                        (CShapeMapEntry 2 "Shape 28" 1
            "Label 17") 22                       (CShapeMapEntry 0 "Shape 15" 1
            "Label 0") 21                        (CShapeMapEntry 8 "Shape 17" 1
            "Label 0") 43                        (CShapeMapEntry 8 "Shape 20" 1
            "Label 0") 50                        (CShapeMapEntry 0 "Shape 32" 1
            "Signal Label") 70                   (CShapeMapEntry 0 "Unique
            RSite" 1 "Label 19") 68                   (CShapeMapEntry 0 "RSite"
            1 "Label 19") 67                          (CShapeMapEntry 0 "ORF" 0
            "ORF Label") 94                           (CShapeMapEntry 8 "Shape
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            1 1                                              (mapper: 15.7067
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..4117
                     /modified_by="mamee"
                     /label="AtCAS9"
     NLS             4130..4150
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     Stop            4151..4153
                     /created_by="mamee"
                     /label="STOP"
     GG              complement(4154..4164)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(4184..4204)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        4684..4788
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    4789..5649
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5820..6408
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6772..6793
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggata agaagtactc tatcggactc gatatcggaa ctaactctgt
       61 gggatgggct gtgatcaccg atgagtacaa ggtgccatct aagaagttca aggttctcgg
      121 aaacaccgat aggcactcta tcaagaaaaa ccttatcggt gctctcctct tcgattctgg
      181 tgaaactgct gaggctacca gactcaagag aaccgctaga agaaggtaca ccagaagaaa
      241 gaacaggatc tgctacctcc aagagatctt ctctaacgag atggctaaag tggatgattc
      301 attcttccac aggctcgaag agtcattcct cgtggaagaa gataagaagc acgagaggca
      361 ccctatcttc ggaaacatcg ttgatgaggt ggcataccac gagaagtacc ctactatcta
      421 ccacctcaga aagaagctcg ttgattctac tgataaggct gatctcaggc tcatctacct
      481 cgctctcgct cacatgatca agttcagagg acacttcctc atcgagggtg atctcaaccc
      541 tgataactct gatgtggata agttgttcat ccagctcgtg cagacctaca accagctttt
      601 cgaagagaac cctatcaacg cttcaggtgt ggatgctaag gctatcctct ctgctaggct
      661 ctctaagtca agaaggcttg agaacctcat tgctcagctc cctggtgaga agaagaacgg
      721 acttttcgga aacttgatcg ctctctctct cggactcacc cctaacttca agtctaactt
      781 cgatctcgct gaggatgcaa agctccagct ctcaaaggat acctacgatg atgatctcga
      841 taacctcctc gctcagatcg gagatcagta cgctgatttg ttcctcgctg ctaagaacct
      901 ctctgatgct atcctcctca gtgatatcct cagagtgaac accgagatca ccaaggctcc
      961 actctcagct tctatgatca agagatacga tgagcaccac caggatctca cacttctcaa
     1021 ggctcttgtt agacagcagc tcccagagaa gtacaaagag attttcttcg atcagtctaa
     1081 gaacggatac gctggttaca tcgatggtgg tgcatctcaa gaagagttct acaagttcat
     1141 caagcctatc ctcgagaaga tggatggaac cgaggaactc ctcgtgaagc tcaatagaga
     1201 ggatcttctc agaaagcaga ggaccttcga taacggatct atccctcatc agatccacct
     1261 cggagagttg cacgctatcc ttagaaggca agaggatttc tacccattcc tcaaggataa
     1321 cagggaaaag attgagaaga ttctcacctt cagaatccct tactacgtgg gacctctcgc
     1381 tagaggaaac tcaagattcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
     1441 gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag tctttcatcg agaggatgac
     1501 caacttcgat aagaaccttc caaacgagaa ggtgctccct aagcactctt tgctctacga
     1561 gtacttcacc gtgtacaacg agttgaccaa ggttaagtac gtgaccgagg gaatgaggaa
     1621 gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt gatctcttgt tcaagaccaa
     1681 cagaaaggtg accgtgaagc agctcaaaga ggattacttc aagaaaatcg agtgcttcga
     1741 ttcagttgag atttctggtg ttgaggatag gttcaacgca tctctcggaa cctaccacga
     1801 tctcctcaag atcattaagg ataaggattt cttggataac gaggaaaacg aggatatctt
     1861 ggaggatatc gttcttaccc tcaccctctt tgaagataga gagatgattg aagaaaggct
     1921 caagacctac gctcatctct tcgatgataa ggtgatgaag cagttgaaga gaagaagata
     1981 cactggttgg ggaaggctct caagaaagct cattaacgga atcagggata agcagtctgg
     2041 aaagacaatc cttgatttcc tcaagtctga tggattcgct aacagaaact tcatgcagct
     2101 catccacgat gattctctca cctttaaaga ggatatccag aaggctcagg tttcaggaca
     2161 gggtgatagt ctccatgagc atatcgctaa cctcgctgga tctcctgcaa tcaagaaggg
     2221 aatcctccag actgtgaagg ttgtggatga gttggtgaag gtgatgggaa ggcataagcc
     2281 tgagaacatc gtgatcgaaa tggctagaga gaaccagacc actcagaagg gacagaagaa
     2341 ctctagggaa aggatgaaga ggatcgagga aggtatcaaa gagcttggat ctcagatcct
     2401 caaagagcac cctgttgaga acactcagct ccagaatgag aagctctacc tctactacct
     2461 ccagaacgga agggatatgt atgtggatca agagttggat atcaacaggc tctctgatta
     2521 cgatgttgat catatcgtgc cacagtcatt cttgaaggat gattctatcg ataacaaggt
     2581 gctcaccagg tctgataaga acaggggtaa gagtgataac gtgccaagtg aagaggttgt
     2641 gaagaaaatg aagaactatt ggaggcagct cctcaacgct aagctcatca ctcagagaaa
     2701 gttcgataac ttgactaagg ctgagagggg aggactctct gaattggata aggcaggatt
     2761 catcaagagg cagcttgtgg aaaccaggca gatcactaag cacgttgcac agatcctcga
     2821 ttctaggatg aacaccaagt acgatgagaa cgataagttg atcagggaag tgaaggttat
     2881 caccctcaag tcaaagctcg tgtctgattt cagaaaggat ttccaattct acaaggtgag
     2941 ggaaatcaac aactaccacc acgctcacga tgcttacctt aacgctgttg ttggaaccgc
     3001 tctcatcaag aagtatccta agctcgagtc agagttcgtg tacggtgatt acaaggtgta
     3061 cgatgtgagg aagatgatcg ctaagtctga gcaagagatc ggaaaggcta ccgctaagta
     3121 tttcttctac tctaacatca tgaatttctt caagaccgag attaccctcg ctaacggtga
     3181 gatcagaaag aggccactca tcgagacaaa cggtgaaaca ggtgagatcg tgtgggataa
     3241 gggaagggat ttcgctaccg ttagaaaggt gctctctatg ccacaggtga acatcgttaa
     3301 gaaaaccgag gtgcagaccg gtggattctc taaagagtct atcctcccta agaggaactc
     3361 tgataagctc attgctagga agaaggattg ggaccctaag aaatacggtg gtttcgattc
     3421 tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt gagaagggaa agagtaagaa
     3481 gctcaagtct gttaaggaac ttctcggaat cactatcatg gaaaggtcat ctttcgagaa
     3541 gaacccaatc gatttcctcg aggctaaggg atacaaagag gttaagaagg atctcatcat
     3601 caagctccca aagtactcac tcttcgaact cgagaacggt agaaagagga tgctcgcttc
     3661 tgctggtgag cttcaaaagg gaaacgagct tgctctccca tctaagtacg ttaactttct
     3721 ttacctcgct tctcactacg agaagttgaa gggatctcca gaagataacg agcagaagca
     3781 acttttcgtt gagcagcaca agcactactt ggatgagatc atcgagcaga tctctgagtt
     3841 ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag gtgttgtctg cttacaacaa
     3901 gcacagagat aagcctatca gggaacaggc agagaacatc atccatctct tcacccttac
     3961 caacctcggt gctcctgctg ctttcaagta cttcgataca accatcgata ggaagagata
     4021 cacctctacc aaagaagtgc tcgatgctac cctcatccat cagtctatca ctggactcta
     4081 cgagactagg atcgatctct cacagctcgg tggtgattca agggctgatc ctaagaagaa
     4141 gaggaaggtt tgatcagtga gaccgaattc tcgccctata gtgagtcgta ttacctggcc
     4201 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4261 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4321 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
     4381 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     4441 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     4501 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     4561 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4621 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg aggtatcatt
     5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6781 taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
