LOCUS       pGG-A-SMBP-B            5740 bp    DNA     circular     28-JUL-2022
DEFINITION  .
ACCESSION   p00131
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        12..3065
                     /modified_by="wacae"
                     /Transferred_From="pSMB"
                     /Transferred_Similarity="100.00%"
                     /label="pSMB"
     GG              complement(3066..3076)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(3096..3116)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        3596..3700
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    3701..4561
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4732..5320
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5684..5705
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tttggtattc atcctcaata tgcaatttat ccatcaacaa tgaacacttg
       61 taattaattt aaatgaaatc tcttagattg tttggctaat ataaattttc ataaaatgtt
      121 ttatataata aatacccgaa aaatggtagt atttaatttc cctccttgtt ataaaatagc
      181 caggtgattc agtaattcac ctgaaccaaa cggtgtacaa cacatatata tatattgtat
      241 actaaaggta attgcgtatc actttgaatt ttatacgtac acttaattag ccataatggg
      301 gattctaaaa taagattata agtttcagaa catgttggta tctctatatg attaaacgct
      361 cattaataag gggtttaatg catgacgtct tcgtgaaggc aacggcacat ctcaataaga
      421 taatattggg ccaacagaaa cttgtaagtt tcataaattg ttttacgaag agattattta
      481 ccgtgagaat ccaatgtttt tatgtgtttc gtgtatctta catggaattt aggaaattaa
      541 aagttaccat tcatatcata ttcgttttta tctttaatag ttttatgtaa ttcgtttggg
      601 gaaaaaagtg agaagaagat aaattttgag gcgaatctca agaagagaga gtgaagtcaa
      661 acgagggacc ttgaattgtg gatttggtgt tggaccatca cgtgctctcc tccacggcct
      721 ttcaggattt tgcttcttct accgcacgtg ccacactcct cctcctacgt accggtttcc
      781 attattgctt ttttcttctt tgcattccaa ttcttcttct tattcttttt gtcaaaggct
      841 ctaaagtctc tatatctatt tgttcgttta gtgaactttt ttatttatat caaatggatt
      901 atttcataaa cttgtaagag ttatatttca ggaaatcata tccacagttt tgaagatcga
      961 tcccccatgc ctttttatat atacaaattc agttatgatt gtttgacgca actgcagata
     1021 aatcatgtgt atacacatta tagggaaaca ttaatagtaa tcctgctatt tgcagaacct
     1081 taagaacgtc tgtgaactga acgtacgtta ttatgtatta atatttcaac tttaaagttt
     1141 aaactaaaaa aaaattagca gaagaaaatt acatgaaaat tgtcttacaa aatgttaaga
     1201 aaaaaaaacg ctagtggaaa tgcgccaata ttacttgcac gttcaatcat atagtataat
     1261 tagctgttca tttaaatccc atagtttctc taacgttttt tttttttttg atgaacactc
     1321 taacgttatt catataacta atatggtaag taaggagcaa atatggaact ttaaggatta
     1381 agatcatttg tagaaaatct tatttctaaa tttccaacga aactgcactg aaattgatgc
     1441 attaaaaaag taagtgggag cataattcaa ggtacgatac atcatttttc ttcccaacat
     1501 ttgactgcaa ttttgagggc atagaagctt caattcctgt tttgttttgt ccttgggata
     1561 cataaaccag atcttgtatt atattatatt taccatgtac tatatagtat tcgaagttca
     1621 ttctcattaa tcgattccta cttgttttca aagcaaatta gtcagcctta ctatataaca
     1681 gctgtaatcc atagtgatta ctgattagta tagttaatcc tcataatcat acttacgtca
     1741 cagtagaaaa taataatatt gtagctatct aatacgtaat atatacacaa ttctcttctt
     1801 cttttttttt gtcaaagata gtatacacaa ttctttagat gtatatctag gtatttatac
     1861 tcgatgattt gtacaaatat gtaaatcgaa gaacttgata ttaattagaa acaaaaaacg
     1921 aaacatagtg tcgaagaaaa agattatttt cggcacaaca tcatgcacaa tcttcattcg
     1981 ctatagacct tctaagccct agctaggcac aacattggac atggaacggt gaatcttcaa
     2041 aattatttgc agatcaccat gtaagagaga tacatatata tagtctagat aggaatgatc
     2101 tgatcatctt agctgatgtt tataccaata tgaataggtt tatatatgtg gcaagaaaaa
     2161 ttatttaatg gaaactgcct atacatatta aagtatttct catatatttc tgtgaccagc
     2221 tttggtttat taaatttaat acaaaataat agtggcactg atccatagaa gccaagcttc
     2281 tctatctttc tctttccaca ctttctttct atctcaaaga agaagaaaga gagagagaga
     2341 gagatagaaa ccctaaacat cgtgcccttg aagctaagat atcttcagct ttgtagaaat
     2401 tcattttgct tacttcattg cgctctctct ctttcattgt cttctacctc ttgcctctaa
     2461 cagaagcaat actttccggc ctccaaggtt cgtttctttc ctcatccatc ttatatcttt
     2521 cattgtcgtc atcatcatct gcatctacat atatatacac acacgcactc cctcacactt
     2581 cctcatatat atcatgtgta cttttcgtct taaacatctc tctattttca tttcttttct
     2641 ttgattactt aagaacatga ctaagtctct atcattaaac ctctcttgtt taagaagcag
     2701 acaaagatcg ttcagtttgc agcaccttca tgtttctctt ggacattcag gttttactta
     2761 tgtgggtata tgtaatagaa gattcatctt ttgttttaaa attaaaggtt aataagttgg
     2821 agcctttgtt cttcgtgact atatgcatgc atttggtaaa cctagggttt tgatttttag
     2881 atctaatata acccaaaccc caattaacac cttgagatta ttggatacat acttgcatgg
     2941 tcaaacccat tcatttctca atttgttaaa acttaaaacc ttcaatataa tgtgtgtttc
     3001 tttctttggg gttaattaat atttcattag ggcaaaggtc gaaaagaaag cgtcaaagtt
     3061 tgcttaacat gagaccgaat tctcgcccta tagtgagtcg tattacctgg ccgtcgtttt
     3121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3181 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3241 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3301 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3361 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3421 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3481 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3541 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3601 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3661 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3721 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3781 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3841 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3901 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3961 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4021 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4081 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4141 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4201 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4261 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4321 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4381 gtttattgct gataaatctg gagccggtga gcgtggctct cgaggtatca ttgcagcact
     4441 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4501 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4561 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4621 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4681 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5401 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5461 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5521 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5581 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5641 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5701 tcacacgagc tatttaggtg acactataga agtgaagctt
//
