LOCUS       pGG-D-UGI-NLS-E         2979 bp    DNA     circular UNA 08-FEB-2023
DEFINITION  .
ACCESSION   urn.local...ng-6q70tzn
VERSION     urn.local...ng-6q70tzn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     CDS             20..268
                     /locus_tag="BI022_gp103"
                     /old_locus_tag="AR9_g104"
                     /note="1UDI_I Dna Glycosylase-Uracil Glycosylase
                     InhibitorProtein Complex of PBS2 phage"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA-glycosylase inhibitor"
                     /protein_id="YP_009283008.1"
                     /db_xref="GI:1070064894"
                     /db_xref="GeneID:29058822"
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVH
                     TAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /modified_by="wacae"
                     /modified_by="mamee"
                     /label="uracil-DNA-glycosylase inhibitor"
     Linker          269..280
                     /created_by="wacae"
                     /modified_by="mamee"
                     /label="SGGS Linker"
     NLS             281..301
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /modified_by="mamee"
                     /label="SV40"
     Stop            302..304
                     /created_by="mamee"
                     /label="STOP"
     GG              complement(305..315)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     primer          complement(335..355)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        835..939
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    940..1800
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1971..2559
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2923..2944
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca ggcggctcaa caaatttatc tgacatcatt gaaaaagaaa caggaaaaca
       61 actagtgatt caagaatcaa ttctaatgtt accagaagaa gtagaggaag taattgggaa
      121 taaaccagaa agtgatattt tagttcatac tgcttatgat gaaagtacag atgaaaatgt
      181 aatgctatta acttcagatg ctccagaata taaaccttgg gctttagtaa ttcaagacag
      241 taatggagaa aataaaatta aaatgttatc cggaggctca cctaagaaga agaggaaggt
      301 ttaactgctg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta
      361 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      421 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      481 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      541 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      601 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      661 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      721 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      781 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1621 tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat tgcagcactg
     1681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     1981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2761 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2821 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2881 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     2941 cacacgagct atttaggtga cactatagaa gtgaagctt
//
