LOCUS       pGG-F-linkerII-G        3232 bp    DNA     circular     08-FEB-2023
DEFINITION  .
ACCESSION   urn.local...47-7a1e0bz
VERSION     urn.local...47-7a1e0bz
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8c-dgycryy"">F</a>"
                     /label="F"
     Linker          12..60
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6k-dgycryx"">Linker II</a>"
                     /label="Linker II"
     GG              complement(60..70)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:84-dgycryy"">G</a>"
                     /label="G"
     misc_feature    complement(845..1378)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8k-dgycryy"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2252..2840
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 ggtctcaact ataagctagg tagtaactag tgcgactgtc tagaatcgcg tatgcgagtg
       61 tattgagacc gagctcctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
      121 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
      181 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
      241 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
      301 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
      361 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
      421 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
      481 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
      541 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
      601 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
      661 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
      721 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
      781 tgataacctt atttttgacg aggggaaatt aggccgggaa gccgatctcg gcttgaacga
      841 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
      901 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
      961 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
     1021 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
     1081 caaacctggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
     1141 caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat
     1201 gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca
     1261 tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac
     1321 ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg
     1381 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1441 cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     1501 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     1561 cattacagtt tacgaaccga acaggcttat gtcaactggg ttcgtgcctt catccgtttc
     1621 cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg
     1681 ctggcgaacg agcgcaaggt ttcgctctcc acgcatcgtc aggcattggc ggccttgctg
     1741 ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct
     1801 cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt
     1861 tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc tagtggttgg
     1921 ctactaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1981 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2041 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2101 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2161 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2641 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2761 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2821 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2881 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2941 ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3001 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3061 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3121 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3181 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatctcgggc cc
//
