LOCUS       pGG-A-LinkerIII-E        3236 bp    DNA     circular     08-FEB-2023
DEFINITION  .
ACCESSION   urn.local...dq-citr5zg
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-11528228|                                                 
            VNTDBDATE|-11528228|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-A-LinkerIII-E|                                         
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            VNTOAUTHORNAME|Invitrogen|                                         
            VNTOAUTHORTEL|(760)603-7200|                                       
            VNTOAUTHORFAX|(760)602-6500|                                       
            VNTOAUTHOREML|tech_service@invitrogen.com|                         
            VNTOAUTHORWWW|http://www.invitrogen.com|                           
            VNTOAUTHORAD1|Invitrogen Corporation|                              
            VNTOAUTHORAD2|1620 Faraday Avenue|                                 
            VNTOAUTHORAD3|Carlsbad, CA 92008, U.S.A.|                          
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     Linker          12..60
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker III"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6l-dgycryx"">Linker III</a>"
                     /label="Linker III"
     GG              complement(61..71)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     misc_feature    complement(846..1379)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8k-dgycryy"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2253..2841
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
ORIGIN      
        1 ggtctcaacc ttaagctagg tagtaactag tgcgactgaa gcttatcgcg tatgcgagtg
       61 ctgctgagac cgagctcctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
      121 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      181 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
      241 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      301 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
      361 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      421 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      481 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      541 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      601 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      661 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      721 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      781 ttgataacct tatttttgac gaggggaaat taggccggga agccgatctc ggcttgaacg
      841 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
      901 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
      961 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc
     1021 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc
     1081 tcaaacctgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca
     1141 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga
     1201 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc
     1261 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca
     1321 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc
     1381 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1441 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     1501 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     1561 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     1621 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     1681 gctggcgaac gagcgcaagg tttccctctc cacgcatcgt caggcattgg cggccttgct
     1741 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcccaagacc
     1801 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     1861 ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg
     1921 gctactaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1981 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2041 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2101 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2161 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2221 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2281 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2341 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2401 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2461 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2641 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2701 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2761 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2821 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2881 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2941 attaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3001 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3061 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3121 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3181 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatcttctc gggccc
//
