LOCUS       pGG-D-AtU6PTA-E         3211 bp    DNA     circular     28-JUL-2022
DEFINITION  .
ACCESSION   urn.local...d5-citr5zd
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11456031|                                                 
            VNTDBDATE|-11456031|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-D-AtU6PTA-E|                                           
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
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                   (274 (CShapeMapEntry 0 "Shape 26" 1 "Label 20") 15          
                    (CShapeMapEntry 8 "Shape 22" 1 "Label 3") 4                
                    (CShapeMapEntry 0 "Shape 33" 1 "Label 21") 29              
                    (CShapeMapEntry 0 "Shape 16" 1 "Label 1") 27               
                    (CShapeMapEntry 2 "Shape 28" 1 "Label 17") 22              
                    (CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21               
                    (CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43               
                    (CShapeMapEntry 8 "Shape 20" 1 "Label 0") 50               
                    (CShapeMapEntry 0 "Shape 32" 1 "Signal Label") 70          
                    (CShapeMapEntry 0 "Unique RSite" 1 "Label 19") 68          
                    (CShapeMapEntry 0 "RSite" 1 "Label 19") 67                 
                    (CShapeMapEntry 0 "ORF" 0 "ORF Label") 94                  
                    (CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88                
                    (CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87               
                    (CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86               
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                    (CShapeMapEntry 8 "Shape 1" 1 "Signal Label")) 67.6449
            67.6449 39 39      0.1 -13986) 1 1 0 1 1                           
                              (mapper: 11.4087 -14.1605 67.6449 67.6449 0.01 10
            -1 3211 3211 1 0 0)     #128=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0
            Nil 1593273782 100)           (CObjectList                         
                                           #129=(CGroupWidget (CWidget 1 (0 0)
            1 2 0 0 Nil -517 100)                  (CObjectList                
                                                    #130=(CAxis                
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            6.27414 1 0.0320093) 0.195365)                              
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                            0.579035 0 -10 6.91848 0.946117 Nil)               
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                                         (LOGFONT 68 26 0 0 400 0 0 0 0 3 2 1
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            "3211 bp" "@L bp"                         16 0 -0.579035 0 -10.8
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                                              #133=(CGroupWidget (CWidget 10 (6
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                            "Chimeric sgRNA" "@N" 1 14.151 5.79035 0 -10       
                                    4.86363 0.783503 #135#)                    
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                           #144=(CGroupWidget                                  
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            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
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            (CStringList) 0 42421752 (CObList))).
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     promoter        14..401
                     /vntifkey=29
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AtU6-26"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:79-dgycryx"">AtU6-26</a>"
                     /label="AtU6-26"
     Scaffold        420..502
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:.:7o-dgycryx"">SpCas9 Scaffold</a>)"
     GG              complement(537..547)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     primer          complement(567..587)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1067..1171
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1172..2032
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2203..2791
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3155..3176
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctctttttt tcttcttctt cgttcataca gttttttttt gtttatcagc
       61 ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat tcggagtttt
      121 tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa
      181 tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa aaggcccctg
      241 ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag tatttcttat
      301 ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag ataagaaaac
      361 gaagctgagt ttatatacag ctagagtcga agtagtgatt ggggtcttcg agaagacctg
      421 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      481 gcaccgagtc ggtgcttttt ttctagaccc agctttcttg tacaaagttg gcattactgc
      541 tgagaccgaa ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg
      601 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      661 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      721 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      781 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
      841 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
      901 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
      961 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     1021 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     1081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     1141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     1201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     1261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     1321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     1381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     1441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     1501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1861 tgataaatct ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga
     1921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     2041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2341 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2581 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2701 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     3001 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     3061 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     3121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag
     3181 ctatttaggt gacactatag aagtgaagct t
//
