LOCUS       pGG-A-GS_linker-C        2712 bp    DNA     circular UNA 28-JUL-2022
DEFINITION  .
ACCESSION   urn.local...4s-cpu7129
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     Linker          12..37
                     /modified_by="mamee"
                     /label="GS_linker"
     GG              complement(38..48)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     primer          complement(68..88)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        568..672
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    673..1533
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1704..2292
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2656..2677
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tctggatcga gtgggggttc tagtggcggc ttgagaccga attctcgccc
       61 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
      301 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
      361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
      481 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      541 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      601 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      661 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      721 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      781 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      841 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      901 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      961 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1021 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1081 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1141 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1201 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1261 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1321 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1381 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1441 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1501 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1561 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1621 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2341 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2401 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2461 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2521 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2581 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2641 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata
     2701 gaagtgaagc tt
//
