LOCUS pGG-A-GS_linker-B 2712 bp DNA circular UNA 28-JUL-2022 DEFINITION . ACCESSION p00012 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; A" /label="A" Linker 12..37 /modified_by="mamee" /label="GS_linker" GG complement(38..48) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" primer complement(68..88) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 568..672 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 673..1533 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 1704..2292 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 2656..2677 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaacc tctggatcga gtgggggttc tagtggcaac atgagaccga attctcgccc 61 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 121 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 181 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg 241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc 301 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct 361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag 481 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg 541 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata 601 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga 661 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 721 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 781 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 841 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 901 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 961 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 1021 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 1081 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 1141 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 1201 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 1261 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 1321 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 1381 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 1441 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 1501 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata 1561 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 1621 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 1681 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 1741 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 1801 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 1861 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 1921 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 1981 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 2041 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 2101 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 2161 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 2221 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 2281 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 2341 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 2401 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 2461 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2521 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 2581 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 2641 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata 2701 gaagtgaagc tt //