LOCUS pGG-D-6xHIS-E 2709 bp DNA circular SYN 13-MAR-2023 DEFINITION . ACCESSION KF718967 VERSION KF718967.1 KEYWORDS . SOURCE Cloning vector pGGD000 (unknown) ORGANISM Cloning vector pGGD000 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3749) AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U. and Forner,J. TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis JOURNAL PLoS ONE 8 (12), E83043 (2013) PUBMED 24376629 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 3749) AUTHORS Forner,J. TITLE Direct Submission JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies, Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230, Heidelberg D-69221, Germany COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##. FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="D" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; D" /label="D" tag 12..34 /cds_type="ORF" /modified_by="mamee" /label="6xHIS*" GG complement(35..45) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="E" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; E" /label="E" primer complement(65..85) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 565..669 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 670..1530 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" rep_origin 1701..2289 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer 2653..2674 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcatca ggtcaccatc accaccatca ctagctgctg agaccgaatt ctcgccctat 61 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg 241 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt 301 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac 361 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 421 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc 481 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 1021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 1081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 1141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 1201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 1261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 1321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 1381 cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 1441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 1501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 2461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 2521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 2581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 2641 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa 2701 gtgaagctt //