LOCUS pGG-D-6xHIS-E 2709 bp DNA circular SYN 13-MAR-2023
DEFINITION .
ACCESSION KF718967
VERSION KF718967.1
KEYWORDS .
SOURCE Cloning vector pGGD000 (unknown)
ORGANISM Cloning vector pGGD000
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 3749)
AUTHORS Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
and Forner,J.
TITLE GreenGate - A Novel, Versatile, and Efficient Cloning System for
Plant Transgenesis
JOURNAL PLoS ONE 8 (12), E83043 (2013)
PUBMED 24376629
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 3749)
AUTHORS Forner,J.
TITLE Direct Submission
JOURNAL Submitted (28-SEP-2013) Centre for Organismal Studies,
Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
Heidelberg D-69221, Germany
COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy
sequencing ##Assembly-Data-END##.
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="D"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; D"
/label="D"
tag 12..34
/cds_type="ORF"
/modified_by="mamee"
/label="6xHIS*"
GG complement(35..45)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="E"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; E"
/label="E"
primer complement(65..85)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 565..669
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 670..1530
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
rep_origin 1701..2289
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer 2653..2674
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcatca ggtcaccatc accaccatca ctagctgctg agaccgaatt ctcgccctat
61 agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
121 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
181 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
241 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
301 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
361 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
421 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
481 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
541 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
601 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
661 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
721 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
781 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
841 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
901 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
961 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
1021 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
1081 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
1141 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
1201 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
1261 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
1321 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
1381 cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
1441 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
1501 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
1561 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
1621 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
1681 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
1741 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
1801 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
1861 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
1921 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
1981 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
2041 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
2101 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
2161 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
2221 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
2281 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
2341 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
2401 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
2461 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
2521 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
2581 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
2641 ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga cactatagaa
2701 gtgaagctt
//