LOCUS       pGG-B-3xMYC-C           2781 bp    DNA     circular SYN 10-FEB-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:87-dgycryy"">B</a>"
                     /label="B"
     tag             14..106
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="3xMYC"
     GG              complement(107..117)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     primer          complement(137..157)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        637..741
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    742..1602
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1773..2361
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2725..2746
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac agcatggagc agaagctgat ctcagaggag gacctggagc agaagctgat
       61 ctcagaggag gacctggagc agaagctgat ctcagaggag gacctgggct tgagaccgaa
      121 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
      181 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      241 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      301 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      361 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      421 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      481 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      541 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      661 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      721 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      781 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      841 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      901 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      961 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1021 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1081 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1141 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1201 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1261 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1321 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1381 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1441 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     1501 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1561 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1621 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1681 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1741 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1801 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1861 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1921 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1981 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2041 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2101 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2161 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2221 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2281 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2341 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2401 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2461 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2521 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2581 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2641 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2701 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     2761 gacactatag aagtgaagct t
//
