LOCUS       pGG-E-G7T-F             2958 bp    DNA     circular     10-MAR-2023
DEFINITION  .
ACCESSION   urn.local...b9-citr5za
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|714504050|                                                 
            VNTDBDATE|-11486158|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-E-G7T-F|                                               
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            0.164644 2.53336                 (274 (CShapeMapEntry 0 "Shape 26"
            1 "Label 20") 15                   (CShapeMapEntry 8 "Shape 22" 1
            "Label 3") 4                         (CShapeMapEntry 0 "Shape 33" 1
            "Label 21") 29                       (CShapeMapEntry 0 "Shape 16" 1
            "Label 1") 27                        (CShapeMapEntry 2 "Shape 28" 1
            "Label 17") 22                       (CShapeMapEntry 0 "Shape 15" 1
            "Label 0") 21                        (CShapeMapEntry 8 "Shape 17" 1
            "Label 0") 43                        (CShapeMapEntry 8 "Shape 20" 1
            "Label 0") 50                        (CShapeMapEntry 0 "Shape 32" 1
            "Signal Label") 70                   (CShapeMapEntry 0 "Unique
            RSite" 1 "Label 19") 68                   (CShapeMapEntry 0 "RSite"
            1 "Label 19") 67                          (CShapeMapEntry 0 "ORF" 0
            "ORF Label") 94                           (CShapeMapEntry 8 "Shape
            2" 1 "Label 4") 88                         (CShapeMapEntry 9 "Shape
            11" 1 "Label 0") 87                        (CShapeMapEntry 8 "Shape
            18" 1 "Label 0") 86                        (CShapeMapEntry 8 "Shape
            19" 1 "Label 0") 85                        (CShapeMapEntry 8 "Shape
            1" 1 "Signal Label")) 67.6449 67.6449 39 39      0.1 -13986) 1 1 0
            1 1                                              (mapper: 11.4087
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                        (LOGFONT 68 26 0 0 400 0 0 0 0 3 2 1 49                
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="E"
     terminator      12..283
                     /modified_by="mamee"
                     /label="tG7"
     GG              complement(284..294)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:83-dgycryy"">F</a>"
                     /label="F"
     primer          complement(314..334)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        814..918
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    919..1779
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1950..2538
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2902..2923
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cccccgatga gctaagctag ctatatcatc aatttatgta ttacacataa
       61 tatcgcactc agtctttcat ctacggcaat gtaccagctg atataatcag ttattgaaat
      121 atttctgaat ttaaacttgc atcaataaat ttatgttttt gcttggacta taatacctga
      181 cttgttattt tatcaataaa tatttaaact atatttcttt caagatggga attaacatct
      241 acaaattgcc ttttcttatc gaccatgtac tcgacggccg agtactatga gaccgaattc
      301 tcgccctata gtgagtcgta ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac
      361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      481 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
      541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
      721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     1261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     1501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     1561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     1621 gccggtgagc gtggctctcg aggtatcatt gcagcactgg ggccagatgg taagccctcc
     1681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     1741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     1801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     2821 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     2881 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgagcta tttaggtgac
     2941 actatagaag tgaagctt
//
