LOCUS       pGG-E-35ST-F            2926 bp    DNA     circular     10-MAR-2023
DEFINITION  .
ACCESSION   urn.local...b8-citr5za
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11486151|                                                 
            VNTDBDATE|-11486159|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-E-35ST-F|                                              
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            1 "Label 20") 15                   (CShapeMapEntry 8 "Shape 22" 1
            "Label 3") 4                         (CShapeMapEntry 0 "Shape 33" 1
            "Label 21") 29                       (CShapeMapEntry 0 "Shape 16" 1
            "Label 1") 27                        (CShapeMapEntry 2 "Shape 28" 1
            "Label 17") 22                       (CShapeMapEntry 0 "Shape 15" 1
            "Label 0") 21                        (CShapeMapEntry 8 "Shape 17" 1
            "Label 0") 43                        (CShapeMapEntry 8 "Shape 20" 1
            "Label 0") 50                        (CShapeMapEntry 0 "Shape 32" 1
            "Signal Label") 70                   (CShapeMapEntry 0 "Unique
            RSite" 1 "Label 19") 68                   (CShapeMapEntry 0 "RSite"
            1 "Label 19") 67                          (CShapeMapEntry 0 "ORF" 0
            "ORF Label") 94                           (CShapeMapEntry 8 "Shape
            2" 1 "Label 4") 88                         (CShapeMapEntry 9 "Shape
            11" 1 "Label 0") 87                        (CShapeMapEntry 8 "Shape
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            1 1                                              (mapper: 11.4087
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="E"
     terminator      12..251
                     /modified_by="mamee"
                     /label="t35S"
     GG              complement(252..262)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:83-dgycryy"">F</a>"
                     /label="F"
     primer          complement(282..302)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        782..886
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    887..1747
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1918..2506
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2870..2891
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cgataactag ctgagcggcc atgctagagt ccgcaaaaat caccagtctc
       61 tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat
      121 aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
      181 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
      241 tccagtgacc tactatgaga ccgaattctc gccctatagt gagtcgtatt acctggccgt
      301 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      361 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      421 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
      481 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
      541 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
      601 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
      661 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
      721 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      961 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1021 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1081 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1141 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1201 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1261 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1321 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1381 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1441 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1501 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1561 tggctggttt attgctgata aatctggagc cggtgagcgt ggctctcgag gtatcattgc
     1621 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1681 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1741 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1801 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1861 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2041 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2761 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2821 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2881 acaatttcac acgagctatt taggtgacac tatagaagtg aagctt
//
