LOCUS       pGG-E-NOST-F            2954 bp    DNA     circular     08-FEB-2023
DEFINITION  .
ACCESSION   urn.local...b7-citr5z9
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11063157|                                                 
            VNTDBDATE|-11486157|                                               
            LSOWNER|                                                           
            VNTNAME|pGG-E-NOST-F|                                              
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            "Label 0") 21                        (CShapeMapEntry 8 "Shape 17" 1
            "Label 0") 43                        (CShapeMapEntry 8 "Shape 20" 1
            "Label 0") 50                        (CShapeMapEntry 0 "Shape 32" 1
            "Signal Label") 70                   (CShapeMapEntry 0 "Unique
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            1 "Label 19") 67                          (CShapeMapEntry 0 "ORF" 0
            "ORF Label") 94                           (CShapeMapEntry 8 "Shape
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FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="E"
     terminator      12..279
                     /modified_by="mamee"
                     /label="tNOS"
     GG              complement(280..290)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:83-dgycryy"">F</a>"
                     /label="F"
     primer          complement(310..330)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        810..914
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    915..1775
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1946..2534
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2898..2919
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg cctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
       61 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
      121 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
      181 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
      241 aaattatcgc gcgcggtgtc atctatgtta ctagatcgaa ctatgagacc gaattctcgc
      301 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      541 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      601 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      661 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      721 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      781 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      841 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      901 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      961 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1021 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1081 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1141 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1201 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1261 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1321 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1381 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1441 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1501 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1561 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1621 gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1681 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1741 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1801 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1861 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1921 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1981 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2041 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2101 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2161 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2221 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2281 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2341 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2401 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2461 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2521 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2581 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2641 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2701 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2761 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2821 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2881 gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta ggtgacacta
     2941 tagaagtgaa gctt
//
