LOCUS pGG-A-pBdEF1a-B 4686 bp DNA circular UNA 23-FEB-2023
DEFINITION .
ACCESSION p00012
KEYWORDS .
SOURCE
ORGANISM .
FEATURES Location/Qualifiers
GG 1..11
/modified_by="wacae"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="A"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; A"
/label="A"
promoter 12..2011
/created_by="lapau"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pBdEF1a.2"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBdEF1a.2"
/label="pBdEF1a.2"
primer_bind 459..478
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="0.1"
/Tm="59.1"
/annotation_group="6688415291435400ad356256-17c4-4e18-80ec
-ecd7fbed510d"
/created_by="primer3"
/Sequence="TCAAAGTCAGATTGCGGCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P119"
primer_bind 1225..1244
/%GC="60.0"
/Hairpin_Tm="41.3"
/Self_Dimer_Tm="None"
/Tm="59.8"
/annotation_group="668841534983210038dae64e-1a86-4525-80b1
-72c57bb81397"
/created_by="primer3"
/Sequence="GTCTTAAGCCCCAGCCTCTC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P120"
primer_bind 1845..1864
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="668841540693610001eaba76-5823-4848-8c0d
-0faf78c8e88a"
/created_by="primer3"
/Sequence="TACATCTGTCCGCCCACCTA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P121"
GG complement(2012..2022)
/created_by="thjac"
/modified_by="thjac"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="B"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; B"
/label="B"
primer complement(2042..2062)
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
/label="GG_SEQ_rev"
promoter 2542..2646
/created_by="mamee"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)"
/label="amp"
misc_feature 2647..3507
/X-Transferred_From="AmpR"
/X-Transferred_Similarity="100.00%"
/modified_by="mamee"
/note="Derived using Geneious Prime 2022.1.1 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="AmpR"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR"
/label="AmpR"
/note="Geneious type: Bacterial Selection"
primer_bind complement(3173..3192)
/%GC="55.0"
/Hairpin_Tm="40.4"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="66884148850148002e5a3764-ccaf-42a0-84d5
-5ae552e98744"
/created_by="primer3"
/Sequence="TACAGGCATCGTGGTGTCAC"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P20"
rep_origin 3678..4266
/created_by="thjac"
/modified_by="mamee"
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
origin of replication"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
ColE1/pMB1/pBR322/pUC Ori (high-copy-number
ColE1/pMB1/pBR322/pUC origin of replication)"
/label="ColE1/pMB1/pBR322/pUC Ori"
primer_bind 3748..3767
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="5.1"
/Tm="60.0"
/annotation_group="668841517440420091e8fcd2-1056-4930-88a7
-60cbd8905ee7"
/created_by="primer3"
/Sequence="TGTTTGCCGGATCAAGAGCT"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P43"
primer_bind complement(3858..3877)
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="58.9"
/annotation_group="6688415059740400ff536362-2a65-4594-9c9d
-92de73f425a4"
/created_by="primer3"
/Sequence="GCGGTGCTACAGAGTTCTTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P6"
primer_bind 4368..4387
/%GC="55.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="60.0"
/annotation_group="6688415234682800c0d116fe-f2d6-42ef-b119
-9e88d222c8ea"
/created_by="primer3"
/Sequence="TACCGCCTTTGAGTGAGCTG"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P39"
primer_bind complement(4619..4638)
/%GC="50.0"
/Hairpin_Tm="None"
/Self_Dimer_Tm="None"
/Tm="59.9"
/annotation_group="6688414825270600109d86be-8af0-41de-8558
-f1b7be22ff7f"
/created_by="primer3"
/Sequence="TCCGCTCACAATTCCACACA"
/Mismatches=0
/#_Off-target_Sites=0
/Off-target_Template="pGG-A-pBdEF1a-B"
/label="P19"
primer 4630..4651
/note="Derived using Geneious Prime 2020.2.2 'Annotate
from Database' based on nucleotide similarity"
/Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
/Transferred_Similarity="100.00%"
/Primary_Match="100.00%; Adjusted-Interval-0:0/0;
GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
/label="GG_SEQ_fw"
ORIGIN
1 ggtctcaacc tagatttgtg attgttcagc tacatgctcc ttgaagcacc tttattctac
61 tataaattag ttcgttggac gagactatta gaccacatgc tagccatgct tgttcgtagt
121 tgtcttcacc gccattgcaa aaattgtcaa taaatattta gagtgggtgg catcagaaaa
181 acatctctag tggactctct tcctatcata gctactcggg ctgtagatag aacgagggca
241 caagagttgg gtggcgtagg tttactcgtg acctcaactc ttttggctgt gtcttacgtc
301 taagatgggt ttggcatgtg agaaacatag gtctaagcaa ttcatgttag ggctgttgca
361 ttgttgttgc atcaaccaaa tgtccagata gcagttcatg ctacatctag ttgaaaaccc
421 tcatcattag gcggaacatg tgttcttttt tagcatagtc aaagtcagat tgcggcactc
481 gctcatccac ggaaagaatt ttccctgtgc aggcatctcg atcaaaagac gcaaattaat
541 ttttgaatag cgatataaca atatctaatt aacgtttctt gttttctgcg aaatgtcttt
601 catcataaaa tgagtcatct cgatgagccc aagtgacata gcccaacacc ccaccccacc
661 aataaaagtg aagaaaacat gttgggaaaa ctataccaag taaaatacga gttgttctaa
721 agaaaaagta aagtacgagt tagatcgcac cctgtcctgg agtgtggctt gatgatccaa
781 ctcctagcat tgtatccctg tttttggatg atgtaactat tatttacaat gaataaagag
841 gtgttttact agtaaaaaaa tcttgagggg aggagaaaat aatggaggtc ttttttcaaa
901 ccgatggact attattttta gtgaaagaga ataatattat tggaaaaatt attctatcca
961 cttattttat attggcagaa tacaaagaat ggtggggtcc acgcggaact tgcggccccc
1021 gaaacctatc gagggcgcgg tacccaagca aggaacggag gaaacttgcg gggcccgaaa
1081 cctagtgata aaaggcatat catccacacg atgaagatct gacggaccat atctcccacc
1141 acggaaagcc atcagacgag gatcagacgg ccaggaagga accctagcgc ccgccggtgc
1201 caatataaag cgccactctc tctcgtctta agccccagcc tctccattcc cctctccctc
1261 tcgccgccgc cgtctccttc tcctactccc ttcgaggtgt gttgttcatc cgtcccgaat
1321 ccatccatcc cctcttcaga tgtgttgttc atggctctaa tagctctaga tctgcttgtt
1381 tgtgttgttt agctctagat ctactcgcgc gcgcttctct ctcgatctcc tgtagaacaa
1441 ttttggttgg ttttttgtgc atatccatgg taattttgtc tgcaatatgg aggaggcttt
1501 ctaagctcct acgtagcatc gatctttaga attccctcgg tttctgttta tttcttcgcg
1561 agggctctct gttatctgta ggagtagctg taagcgcggt tcgttacgga ttaatcgtca
1621 tgcttagttg aacctatcgg tcgaaggatt tgtgtgggtt gtcgtgtaga attgacacca
1681 tctacttact gtactgatat gccgatctgt aggatactct tcattacttt tgtttactgc
1741 tagttgtggt gtagatttag cattctcaaa cccatgctgt agcgtttcta atattgttac
1801 atagatctac cggtgcctgt taattgtatt cgatcgggcg tttctacatc tgtccgccca
1861 cctagtttta tatgtggtaa tcaaaattgc gttgacttcg tgatgctgtc tgtgtactgt
1921 ttttaatcgc tcttacttag atgatcaaca tggtgatggt tacgatttac tgttttctaa
1981 tccctgttac ttcgatgctg cagtttcagc caacatgaga ccgaattctc gccctatagt
2041 gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
2101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
2161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
2221 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
2281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
2341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
2401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
2461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
2521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
2581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
2641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
2701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
2761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
2821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
2881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
2941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
3001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
3061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
3121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
3181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
3241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
3301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
3361 ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
3421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
3481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
3541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
3601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
3661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
3721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
3781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
3841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
3901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
3961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
4141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
4201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
4261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
4321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
4381 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
4441 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
4501 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
4561 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
4621 tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg
4681 aagctt
//