LOCUS pGG-A-pBdEF1a-B 4686 bp DNA circular UNA 23-FEB-2023 DEFINITION . ACCESSION p00012 KEYWORDS . SOURCE ORGANISM . FEATURES Location/Qualifiers GG 1..11 /modified_by="wacae" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="A" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; A" /label="A" promoter 12..2011 /created_by="lapau" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pBdEF1a.2" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pBdEF1a.2" /label="pBdEF1a.2" primer_bind 459..478 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="0.1" /Tm="59.1" /annotation_group="6688415291435400ad356256-17c4-4e18-80ec -ecd7fbed510d" /created_by="primer3" /Sequence="TCAAAGTCAGATTGCGGCAC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P119" primer_bind 1225..1244 /%GC="60.0" /Hairpin_Tm="41.3" /Self_Dimer_Tm="None" /Tm="59.8" /annotation_group="668841534983210038dae64e-1a86-4525-80b1 -72c57bb81397" /created_by="primer3" /Sequence="GTCTTAAGCCCCAGCCTCTC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P120" primer_bind 1845..1864 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="668841540693610001eaba76-5823-4848-8c0d -0faf78c8e88a" /created_by="primer3" /Sequence="TACATCTGTCCGCCCACCTA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P121" GG complement(2012..2022) /created_by="thjac" /modified_by="thjac" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="B" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; B" /label="B" primer complement(2042..2062) /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)" /label="GG_SEQ_rev" promoter 2542..2646 /created_by="mamee" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (AmpR)" /label="amp" misc_feature 2647..3507 /X-Transferred_From="AmpR" /X-Transferred_Similarity="100.00%" /modified_by="mamee" /note="Derived using Geneious Prime 2022.1.1 'Annotate from Database' based on nucleotide similarity" /Transferred_From="AmpR" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR" /label="AmpR" /note="Geneious type: Bacterial Selection" primer_bind complement(3173..3192) /%GC="55.0" /Hairpin_Tm="40.4" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="66884148850148002e5a3764-ccaf-42a0-84d5 -5ae552e98744" /created_by="primer3" /Sequence="TACAGGCATCGTGGTGTCAC" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P20" rep_origin 3678..4266 /created_by="thjac" /modified_by="mamee" /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ColE1/pMB1/pBR322/pUC Ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of replication)" /label="ColE1/pMB1/pBR322/pUC Ori" primer_bind 3748..3767 /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="5.1" /Tm="60.0" /annotation_group="668841517440420091e8fcd2-1056-4930-88a7 -60cbd8905ee7" /created_by="primer3" /Sequence="TGTTTGCCGGATCAAGAGCT" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P43" primer_bind complement(3858..3877) /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="58.9" /annotation_group="6688415059740400ff536362-2a65-4594-9c9d -92de73f425a4" /created_by="primer3" /Sequence="GCGGTGCTACAGAGTTCTTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P6" primer_bind 4368..4387 /%GC="55.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="60.0" /annotation_group="6688415234682800c0d116fe-f2d6-42ef-b119 -9e88d222c8ea" /created_by="primer3" /Sequence="TACCGCCTTTGAGTGAGCTG" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P39" primer_bind complement(4619..4638) /%GC="50.0" /Hairpin_Tm="None" /Self_Dimer_Tm="None" /Tm="59.9" /annotation_group="6688414825270600109d86be-8af0-41de-8558 -f1b7be22ff7f" /created_by="primer3" /Sequence="TCCGCTCACAATTCCACACA" /Mismatches=0 /#_Off-target_Sites=0 /Off-target_Template="pGG-A-pBdEF1a-B" /label="P19" primer 4630..4651 /note="Derived using Geneious Prime 2020.2.2 'Annotate from Database' based on nucleotide similarity" /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw" /Transferred_Similarity="100.00%" /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)" /label="GG_SEQ_fw" ORIGIN 1 ggtctcaacc tagatttgtg attgttcagc tacatgctcc ttgaagcacc tttattctac 61 tataaattag ttcgttggac gagactatta gaccacatgc tagccatgct tgttcgtagt 121 tgtcttcacc gccattgcaa aaattgtcaa taaatattta gagtgggtgg catcagaaaa 181 acatctctag tggactctct tcctatcata gctactcggg ctgtagatag aacgagggca 241 caagagttgg gtggcgtagg tttactcgtg acctcaactc ttttggctgt gtcttacgtc 301 taagatgggt ttggcatgtg agaaacatag gtctaagcaa ttcatgttag ggctgttgca 361 ttgttgttgc atcaaccaaa tgtccagata gcagttcatg ctacatctag ttgaaaaccc 421 tcatcattag gcggaacatg tgttcttttt tagcatagtc aaagtcagat tgcggcactc 481 gctcatccac ggaaagaatt ttccctgtgc aggcatctcg atcaaaagac gcaaattaat 541 ttttgaatag cgatataaca atatctaatt aacgtttctt gttttctgcg aaatgtcttt 601 catcataaaa tgagtcatct cgatgagccc aagtgacata gcccaacacc ccaccccacc 661 aataaaagtg aagaaaacat gttgggaaaa ctataccaag taaaatacga gttgttctaa 721 agaaaaagta aagtacgagt tagatcgcac cctgtcctgg agtgtggctt gatgatccaa 781 ctcctagcat tgtatccctg tttttggatg atgtaactat tatttacaat gaataaagag 841 gtgttttact agtaaaaaaa tcttgagggg aggagaaaat aatggaggtc ttttttcaaa 901 ccgatggact attattttta gtgaaagaga ataatattat tggaaaaatt attctatcca 961 cttattttat attggcagaa tacaaagaat ggtggggtcc acgcggaact tgcggccccc 1021 gaaacctatc gagggcgcgg tacccaagca aggaacggag gaaacttgcg gggcccgaaa 1081 cctagtgata aaaggcatat catccacacg atgaagatct gacggaccat atctcccacc 1141 acggaaagcc atcagacgag gatcagacgg ccaggaagga accctagcgc ccgccggtgc 1201 caatataaag cgccactctc tctcgtctta agccccagcc tctccattcc cctctccctc 1261 tcgccgccgc cgtctccttc tcctactccc ttcgaggtgt gttgttcatc cgtcccgaat 1321 ccatccatcc cctcttcaga tgtgttgttc atggctctaa tagctctaga tctgcttgtt 1381 tgtgttgttt agctctagat ctactcgcgc gcgcttctct ctcgatctcc tgtagaacaa 1441 ttttggttgg ttttttgtgc atatccatgg taattttgtc tgcaatatgg aggaggcttt 1501 ctaagctcct acgtagcatc gatctttaga attccctcgg tttctgttta tttcttcgcg 1561 agggctctct gttatctgta ggagtagctg taagcgcggt tcgttacgga ttaatcgtca 1621 tgcttagttg aacctatcgg tcgaaggatt tgtgtgggtt gtcgtgtaga attgacacca 1681 tctacttact gtactgatat gccgatctgt aggatactct tcattacttt tgtttactgc 1741 tagttgtggt gtagatttag cattctcaaa cccatgctgt agcgtttcta atattgttac 1801 atagatctac cggtgcctgt taattgtatt cgatcgggcg tttctacatc tgtccgccca 1861 cctagtttta tatgtggtaa tcaaaattgc gttgacttcg tgatgctgtc tgtgtactgt 1921 ttttaatcgc tcttacttag atgatcaaca tggtgatggt tacgatttac tgttttctaa 1981 tccctgttac ttcgatgctg cagtttcagc caacatgaga ccgaattctc gccctatagt 2041 gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 2101 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 2161 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg 2221 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca 2281 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg 2341 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 2401 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc 2461 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 2521 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 2581 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 2641 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 2701 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 2761 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 2821 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 2881 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 2941 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 3001 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 3061 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 3121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 3181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 3241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 3301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 3361 ggctctcgag gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 3421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 3481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 3541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 3601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 3661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 3841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 4021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 4081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 4141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 4201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 4261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 4321 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 4381 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 4441 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 4501 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 4561 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 4621 tgtggaattg tgagcggata acaatttcac acgagctatt taggtgacac tatagaagtg 4681 aagctt //