LOCUS       pGG-C-BP-Cas9-BP*-D        6912 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...a1-ew4hf81
VERSION     urn.local...a1-ew4hf81
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     misc_feature    14..16
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     NLS             17..70
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP (<a
                     href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:33/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP*
                     (<a href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /label="BP"
     CDS             77..4180
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /label="CAS9"
     NLS             4187..4237
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     GG              complement(4238..4248)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="D"
     primer          complement(4268..4288)
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     misc_feature    4768..5733
                     /modified_by="wacae"
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5904..6492
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6856..6877
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatgaaac ggacagccga cggaagcgag ttcgagtcac ctaagaagaa
       61 gaggaaggtt ggctcgatgg ataagaagta ctctatcgga ctcgatatcg gaactaactc
      121 tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt tcaaggttct
      181 cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc
      241 tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt acaccagaag
      301 aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta aagtggatga
      361 ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga agcacgagag
      421 gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt accctactat
      481 ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca ggctcatcta
      541 cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg gtgatctcaa
      601 ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct acaaccagct
      661 tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc tctctgctag
      721 gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg agaagaagaa
      781 cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact tcaagtctaa
      841 cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg atgatgatct
      901 cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg ctgctaagaa
      961 cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga tcaccaaggc
     1021 tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc tcacacttct
     1081 caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct tcgatcagtc
     1141 taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt tctacaagtt
     1201 catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga agctcaatag
     1261 agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc atcagatcca
     1321 cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat tcctcaagga
     1381 taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg tgggacctct
     1441 cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa ccatcacccc
     1501 ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca tcgagaggat
     1561 gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact ctttgctcta
     1621 cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg agggaatgag
     1681 gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct tgttcaagac
     1741 caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa tcgagtgctt
     1801 cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg gaacctacca
     1861 cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa acgaggatat
     1921 cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga ttgaagaaag
     1981 gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga agagaagaag
     2041 atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg ataagcagtc
     2101 tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa acttcatgca
     2161 gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc aggtttcagg
     2221 acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg caatcaagaa
     2281 gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg gaaggcataa
     2341 gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga agggacagaa
     2401 gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg gatctcagat
     2461 cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct acctctacta
     2521 cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca ggctctctga
     2581 ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta tcgataacaa
     2641 ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa gtgaagaggt
     2701 tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca tcactcagag
     2761 aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg ataaggcagg
     2821 attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg cacagatcct
     2881 cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg aagtgaaggt
     2941 tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat tctacaaggt
     3001 gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
     3061 cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg attacaaggt
     3121 gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa
     3181 gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc tcgctaacgg
     3241 tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga
     3301 taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg tgaacatcgt
     3361 taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc ctaagaggaa
     3421 ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg gtggtttcga
     3481 ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa
     3541 gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt catctttcga
     3601 gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga aggatctcat
     3661 catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga ggatgctcgc
     3721 ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt acgttaactt
     3781 tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata acgagcagaa
     3841 gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc agatctctga
     3901 gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa
     3961 caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc tcttcaccct
     4021 taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg ataggaagag
     4081 atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta tcactggact
     4141 ctacgagact aggatcgatc tctcacagct cggtggtgat tcaggtaaaa gaaccgccga
     4201 cggcagcgaa ttcgagccta agaagaagag gaaggtttca gtgagaccga attctcgccc
     4261 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     4441 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4501 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4561 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4621 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4681 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4741 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4801 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4861 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4921 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4981 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5041 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5101 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5161 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5221 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5281 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5341 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5401 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5461 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5521 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5581 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5641 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5701 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6781 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6841 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata
     6901 gaagtgaagc tt
//
