LOCUS       pEN-L1-mCherry-L2        3255 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...wt-ejuyuzr
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|665408589|                                                 
            VNTDBDATE|707322776|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L1-mCherry-L2|                                         
            VNTAUTHORNAME|Mansou Karimi|                                       
            VNTAUTHORTEL|0032-9-3313838|                                       
            VNTAUTHOREML|manka@psb.vib-ugent.be|                               
            VNTOAUTHORNAME|Computer Group|                                     
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                  (CWidget 1 (10 #10# 0) 1 2 0 0 Nil -1060 100)                
                    (CObjectList                                               
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                                 (LOGPEN 0 10 0) 8 1 1.5842 1.9 0.123113 1)    
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                                       (CWidget 0 (0 0) 1 2 0 0 #214# 30933172
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            (2434)" "@N (@S)" 5 -14.5413 0 0                                -10
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            #245=(CGroupWidget                                                 
                  (CWidget 1 (10 #12# 0) 1 2 0 0 Nil -355 100)                 
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            1.15807 0.611111 0                                "{\\i Eco}RV
            (104)" "@N (@S)" 5 5.174 12.7388                                0
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            #248=(CGroupWidget                                                 
                  (CWidget 1 (10 #15# 0) 1 2 0 0 Nil -277 100)                 
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            (343)" "@N (@S)" 5 10.9193                                9.26456 0
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                                                           #251=(CGroupWidget  
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            #31# 0) 1 2 0 0 Nil -279 100)                         (CObjectList 
                                                                  
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                                                       #254=(CGroupWidget      
                                                             (CWidget 1 (10
            #33# 0) 1 2 0 0 Nil -122 100)                         (CObjectList 
                                                                  
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            0                                "{\\i Esp}3I (702)" "@N (@S)" 5
            14.3529                                2.31614 0 -10 2.98618
            0.724371 #255#))                         (CObjectList))            
                                               #257=(CGroupWidget              
                                                     (CWidget 2 (10 #26# 0) 1 2
            0 0 Nil -1297 100)                         (CObjectList            
                                                        #258=(CScratch         
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                                "{\\i Acs}I (299)" "@N (@S)" 5 9.64944         
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                                   (CWidget 2 (1 #26# 2) 1 2 0 0 #149# 0 100)  
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            #262=(CGroupWidget                                                 
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            (CObjectList))                         #267=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -435 100)                  (CObjectList)
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            (CWidget 12 (0 0) 1 2 0 0 Nil -827 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList #269=(XLATFRAGMENT 2536 235 0 1 2537 707))    
            (CObList #270=(AnlzFontMaskItem 2534 2535 4)                       
             #271=(AnlzFontMaskItem 2536 2536 4)                               
             #272=(AnlzXLATItem 2536 3243 2 2536 3243)) 1 (CObList)) (CObList) 
              1633776737 (CStringList) 1734435169 1734436705 (CObList))).
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
     misc_feature    48
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             103..810
                     /label="mCherry"
     primer_bind     347..366
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="66800825619919006ae12041-df0c-4cff-8ea1
                     -518ccae29922"
                     /created_by="primer3"
                     /Sequence="CCGACATCCCCGACTACTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-mCherry-L2"
                     /label="P103"
     attL2           complement(812..911)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
     promoter        complement(930..947)
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="T7 promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:yz-f3tbldq"">T7 promoter</a>"
                     /label="T7 promoter"
     primer          complement(952..968)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     misc_feature    1022..1023
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Replacement"
     promoter        1030..1080
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1081..1890
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1106..1125
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="6680082260211400a0fc9fe2-4831-4b63-940a
                     -e290a1fde4bf"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-mCherry-L2"
                     /label="P44"
     primer_bind     1740..1759
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="66800824662994009460345f-2bb5-44e5-b95b
                     -ff730dddae91"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-mCherry-L2"
                     /label="P42"
     rep_origin      2037..2625
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     2525..2544
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="668008231755880073a5a0a4-5e1c-4c21-94f7
                     -52f972402f3a"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-mCherry-L2"
                     /label="P45"
     primer_bind     3133..3152
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6680082375158100c120fbca-185e-4e79-abae
                     -5f4eddded0ae"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-mCherry-L2"
                     /label="P46"
     primer          3204..3220
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3224..3239
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taatggtgag caagggcgag
      121 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      181 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      241 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      301 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      361 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      421 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      481 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      541 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      601 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      661 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
      721 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
      781 tccaccggcg gcatggagga gctgtacaag tacccagctt tcttgtacaa agttggcatt
      841 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      901 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
      961 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1021 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1081 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1141 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1201 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1261 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1321 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1381 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1441 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1501 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1561 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1621 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1681 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1741 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1801 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1861 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     1921 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     1981 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2041 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2101 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2161 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2221 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2281 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2401 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2641 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2701 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2761 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2821 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2881 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     2941 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     3001 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3061 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3121 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3181 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3241 cttaagctcg ggccc
//
