LOCUS       pH7m24GW-FAST          13967 bp    DNA     circular SYN 29-AUG-2022
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    283..1074
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1320..1908
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1944..2204)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2578..2772
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2838..3911)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4340..4969)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6155..6385
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     promoter        6387..7829
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pOLE1"
                     /Transferred_Similarity="99.93%"
                     /Primary_Match="99.93%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6w-dgycryx"">pOLE1</a>"
                     /label="pOLE1"
     CDS             join(7830..8182,8423..8588)
                     /modified_by="thjac"
                     /X-Transferred_From="OLE1"
                     /X-Transferred_Similarity="100.00%"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="99.42%"
                     /Primary_Match="99.42%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; OLE1 (<a
                     href=""urn:local:.:91-dgycryy"">OLE1 gene</a>)"
                     /label="OLE1"
     intron          8183..8422
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OLE1 gene"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:.:91-dgycryy"">OLE1 gene</a>)"
                     /label
     CDS             8643..9533
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Egfp"
                     /Transferred_Similarity="99.72%"
                     /Primary_Match="99.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hy-e4twm0o"">Egfp</a>"
                     /label="Egfp"
     terminator      9549..9774
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S v2"
                     /Transferred_Similarity="99.56%"
                     /Primary_Match="99.56%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:lk-f0ukkf4"">t35S v2</a>)"
                     /label="t35S"
     attR4           9799..9922
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     promoter        9947..9977
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    10031..10690
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     UTR             10713..11031
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     CDS             11032..11337
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(11378..11502)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      11560..11785
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S v2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:lk-f0ukkf4"">t35S v2</a>)"
                     /label="t35S"
     Plant_Selection 11850..13614
                     /modified_by="mamee"
                     /X-Transferred_From="Hygromycin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
                     (<a href=""urn:local:.:l3-f0p6xgl"">Hygromycin</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hygro"
     promoter        11850..12122
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:ip-f0oyhrv"">pNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     CDS             12137..13162
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hyg"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:90-dgycryy"">Hyg</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hyg"
     terminator      13347..13614
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     T-DNA_border    13635..13967
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
      301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
     6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     6361 atctgatcca agctcaagct aagctctact tagatcaaca cataaaagtt agtaagtgaa
     6421 gaaccacaac aacaacacta gattcatctt caagtgtatg taggtatagt aacatgaaca
     6481 agaacagact caagtacaag atcgcatacg aaaatggaaa tggcaatgtc acttccacat
     6541 aatcaaacac gaatcctcat atcaacaagg cctgagattc taactagctc ataacaactt
     6601 agccaatagt tacttgagac taccaaatgt atgtagaact aaagactaag ggacagagag
     6661 ttcgtctaaa caggtgaatc tagtcgttgt tatctaataa acaattcagc cccaaatgca
     6721 gaacacacat agagctctct attgattcaa attacgatct gatactgata acgtctagat
     6781 ttttagggtt aaagcaatca atcacctgac gattcaaggt ggttggatca tgacgattcc
     6841 agaaaacatc aagcaagctc tcaaagctac actctttggg atcatactga actctaacaa
     6901 cctcgttatg tcccgtagtg ccagtacaga catcctcgta actcggattg tgcacgatgc
     6961 catggctata cccaacctcg gtcttggtca caccaggaac tctctggtaa gctagctcca
     7021 ctccccagaa acaaccggcg ccaaattgcg cgaattgctg acctgaagac ggaacatcat
     7081 cgtcgggtcc ttgggcgatt gcggcggaag atgggtcagc ttgggcttga ggacgagacc
     7141 cgaatccgag tctgttgaaa aggttgttca ttggggattt gtatacggag attggtcgtc
     7201 gagaggtttg agggaaagga caaatgggtt tggctctgga gaaagagagt gcggctttag
     7261 agagagaatt gagaggttta gagagagatg cggcggcgat gagcggagga gagacgacga
     7321 ggacctgcat tatcaaagca gtgacgtggt gaaatttgga acttttaaga ggcagataga
     7381 tttattattt gtatccattt tcttcattgt tctagaatgt cgcggaacaa attttaaaac
     7441 taaatcctaa atttttctaa ttttgttgcc aatagtggat atgtgggccg tatagaagga
     7501 atctattgaa ggcccaaacc catactgacg agcccaaagg ttcgttttgc gttttatgtt
     7561 tcggttcgat gccaacgcca cattctgagc taggcaaaaa acaaacgtgt ctttgaatag
     7621 actcctctcg ttaacacatg cagcggctgc atggtgacgc cattaacacg tggcctacaa
     7681 ttgcatgatg tctccattga cacgtgactt ctcgtctcct ttcttaatat atctaacaaa
     7741 cactcctacc tcttccaaaa tatatacaca tctttttgat caatctctca ttcaaaatct
     7801 cattctctct agtaaacaag aacaaaaaaa tggcggatac agctagagga acccatcacg
     7861 atatcatcgg cagagaccag tacccgatga tgggccgaga ccgagaccag taccagatgt
     7921 ccggacgagg atctgactac tccaagtcta ggcagattgc taaagctgca actgctgtca
     7981 cagctggtgg ttccctcctt gttctctcca gccttaccct tgttggaact gtcatagctt
     8041 tgactgttgc aacacctctg ctcgttatct tcagcccaat ccttgtcccg gctctcatca
     8101 cagttgcact cctcatcacc ggttttcttt cctctggagg gtttggcatt gccgctataa
     8161 ccgttttctc ttggatttac aagtaagcac acatttatca tcttacttca taattttgtg
     8221 caatatgtgc atgcatgtgt tgagccagta gctttggatc aatttttttg gtcgaataac
     8281 aaatgtaaca ataagaaatt gcaaattcta gggaacattt ggttaactaa atacgaaatt
     8341 tgacctagct agcttgaatg tgtctgtgta tatcatctat ataggtaaaa tgcttggtat
     8401 gatacctatt gattgtgaat aggtacgcaa cgggagagca cccacaggga tcagacaagt
     8461 tggacagtgc aaggatgaag ttgggaagca aagctcagga tctgaaagac agagctcagt
     8521 actacggaca gcaacatact ggtggggaac atgaccgtga ccgtactcgt ggtggccagc
     8581 acactactca agggtgggcg cgccgaccca gctttcttgt acaaagtggt gatatcccgc
     8641 ggatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     8701 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     8761 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     8821 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     8881 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     8941 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     9001 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     9061 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     9121 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     9181 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     9241 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     9301 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacgaac
     9361 ccggcatgtt acgtcctgta gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt
     9421 gggcattcag tctggatcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     9481 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgaccatggc
     9541 ggccgggagg gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct
     9601 ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct
     9661 tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt
     9721 aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc
     9781 atgcgagagc ttgagctcca actttgtata gaaaagttga acgagaaacg taaaatgata
     9841 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     9901 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     9961 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
    10021 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
    10081 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
    10141 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
    10201 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
    10261 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
    10321 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
    10381 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
    10441 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
    10501 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
    10561 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
    10621 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
    10681 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
    10741 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
    10801 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
    10861 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
    10921 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
    10981 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
    11041 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
    11101 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
    11161 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
    11221 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
    11281 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
    11341 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
    11401 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
    11461 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgg
    11521 cggccgggag catgcgacgt cgatctaact gactagccgc ggccatgcta gagtccgcaa
    11581 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
    11641 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
    11701 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
    11761 tcctaaaacc aaaatccagt gacctgcagg catgcgacgt cgggccctct agaggatccc
    11821 cgggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt atgacccccg
    11881 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga
    11941 gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
    12001 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
    12061 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
    12121 aactcgaatc ccccctatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
    12181 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
    12241 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
    12301 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
    12361 ggaagtgctt gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc
    12421 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc ttcagccggt
    12481 cgcggaggct atggatgcta tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
    12541 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
    12601 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
    12661 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
    12721 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
    12781 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
    12841 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
    12901 gcttgcagga tcgccaaggc tccgggcgta tatgctccgc attggtcttg accaactcta
    12961 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
    13021 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
    13081 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
    13141 tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg
    13201 cctcagccag caactcgcgc gagcctagca aggcaaatgc gagagaacgg ccttacgctt
    13261 ggtggcacag ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc gggagggccg
    13321 gtcgcagtga ttcaggaatt aattccctag agtcaagcag atcgttcaaa catttggcaa
    13381 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
    13441 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
    13501 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
    13561 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca
    13621 tgcaagctga taattcaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
    13681 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
    13741 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
    13801 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
    13861 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
    13921 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatca
//
