LOCUS       pXK7FNF2               11211 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...23e-dx41dff
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|473175269|                                                 
            VNTDBDATE|473175269|                                               
            LSOWNER|                                                           
            VNTNAME|pXK7FNF2,0|                                                
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|mansour.karimi@psb.ugent.be|                          
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                                        (LOGPEN 0 0 8437824) 1                 
                                        (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sal}I (9616)" "@N (@S)" 5
            -13.9386                                9.26456 0 -10 2.92705
            0.694805 #352#))                         (CObjectList))            
                                               #354=(CGroupWidget              
                                                     (CWidget 2 (10 #23# 0) 1 2
            0 0 Nil 12559328 100)                         (CObjectList         
                                                           #355=(CScratch      
                                                                 (CWidget 1 (1
            #23# 1) 1 2 0 0 #231# 0 100)                                (LOGPEN
            0 10 0) 8 1 5.29726 1.9 0.123113 1)                         
            #356=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #259# 32139796 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Sph}I (1742)" "@N (@S)" 5 14.7658         
                                  8.10649 0 -10 3.11923 0.694805 #355#)        
                             #357=(CScratch                                    
                                   (CWidget 2 (1 #23# 2) 1 2 0 0 #231# 0 100)  
                                         (LOGPEN 0 10 0) 8 1 1.63346 1.9
            0.123113 1)                          #358=(CLabel                  
                                                       (CWidget 0 (0 0) 1 2 0 0
            #259# 32142292 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Sph}I (8278)" "@N (@S)" 5 -16.8131                           
                -2.31614 0 -10 3.11923 0.694805 #357#))                        
            (CObjectList))) (CObjectList))                        
            #359=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 12559328 100)    
                         (CObjectList) (CObjectList))                          
                   #360=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 327685 100)
                             (CObjectList) (CObjectList))) (CObjectList)))     
               (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
            1735357044    (CStringList) 544370548 2083664691 (CObList))).
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="6069676270406400f311e96e-9f45-4ab5-81fb
                     -43fe3cd54f2b"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     771..790
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="6069676566179600ff475f39-3497-4cad-8894
                     -a376e26b24b4"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P24"
     primer_bind     1384..1403
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="6069676622391100e4399e56-4de0-489f-828f
                     -f53a9575ce57"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P25"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1725..1729
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     terminator      complement(1772..1997)
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     1899..1922
                     /%GC="45.8"
                     /Hairpin_Tm="32.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="606967743042760018cf7b3a-8457-41a3-91f2
                     -7b80f5e727d2"
                     /created_by="primer3"
                     /Sequence="CCTTATCTGGGAACTACTCACACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P65"
     misc_feature    1989^1990
                     /Original_Bases="CATGGCCGCTCCCGGCCGC"
                     /label="CATGGCCGCTCCCGGCCGC"
                     /note="Geneious type: Editing History Deletion"
     primer          2016..2032
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          2036..2051
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     CDS             complement(2082..2798)
                     /label="GFP"
     CDS             complement(2826..3542)
                     /label="GFP"
     NLS             complement(3543..3587)
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NLS c2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; c2 NLS
                     (<a href=""urn:local:.:9c-dgycryy"">NLS c2</a>)"
                     /modified_by="mamee"
                     /label="c2"
     primer_bind     complement(3576..3595)
                     /%GC="55.0"
                     /Hairpin_Tm="42.3"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="60696774885393009341c4d3-116b-43f6-b593
                     -cda5892fa02c"
                     /created_by="primer3"
                     /Sequence="GCTCCATGCAGCCTTCTCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P66"
     misc_feature    3617
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        complement(3646..4692)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     misc_feature    3712^3713
                     /Original_Bases="CGGATCGTC"
                     /label="CGGATCGTC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    3714
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3716
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3911
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     3941..3962
                     /%GC="45.5"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="12.2"
                     /Tm="59.9"
                     /annotation_group="606967650978200041ba6ff7-d3f8-4d46-adfc
                     -956e240d6219"
                     /created_by="primer3"
                     /Sequence="CGCAATGATGGCATTTGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P18"
     primer_bind     complement(4004..4023)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="60.2"
                     /annotation_group="6069676394220500a7a33470-abf9-45f7-840d
                     -c070f2bdd95c"
                     /created_by="primer3"
                     /Sequence="CGGATTCCATTGCCCAGCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P11"
     misc_feature    4660..4672
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    4741..4742
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          complement(4783..4799)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     T-DNA_border    4827..5027
                     /modified_by="mamee"
                     /label="RB"
     primer_bind     4848..4867
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6069676451573300a33d626f-d93e-4b95-bdb8
                     -e8d90871e384"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P14"
     misc_feature    4942
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5025..5054
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(5110..5129)
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="6069677027213700a176bfef-1515-4c71-b75d
                     -78012b5133e4"
                     /created_by="primer3"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P37"
     primer_bind     5676..5694
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="60696758685222003b3d555d-334f-44ca-ae0c
                     -ad6da906e2ef"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P1"
     regulatory      6243..6872
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     6468..6487
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6069675928044200974b8edf-0a3a-4ff1-832e
                     -e0b490eab387"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P2"
     primer_bind     7265..7283
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="606967598499360063ffea3d-4891-423c-9266
                     -c1488999b018"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P3"
     regulatory      7301..8374
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    7305
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     8069..8086
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="6069676041888100baa7967b-3007-4819-bb56
                     -f68fbfb83460"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P4"
     rep_origin      8440..8634
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     8869..8888
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60696760981881004e8fade1-7597-4ad7-9fab
                     -7f09f02e84ac"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P5"
     regulatory      9008..9268
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(9304..9892)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     9693..9712
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="6069676155331800b3e757cf-d7e0-498a-828f
                     -14adf85019d0"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P6"
     misc_feature    10078^10079
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10090^10091
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10095..10098
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(10138..10929)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     10465..10484
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="6069676213585100df28a129-ac68-4fd8-bb16
                     -e5b9ea0c3b4d"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7FNF2"
                     /label="P7"
     Promoter        complement(10930..11204)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    11064^11065
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11072^11073
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11143..11144
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    11147^11148
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11180^11181
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgagc tctcccatat ggtcgacctg caggtcactg gattttggtt ttaggaatta
     1801 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     1861 ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca
     1921 cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg tgatttttgc
     1981 ggactctagc atggccgcgg ctagtcagtt agatcgtttt cccagtcacg acgttgtaaa
     2041 acgacggcca gtgaattatc aactatgtat aataaagttg tcttgtacag ctcgtccatg
     2101 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     2161 ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
     2221 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
     2281 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
     2341 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     2401 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     2461 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     2521 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     2581 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
     2641 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
     2701 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     2761 accaccccgg tgaacagctc ctcgcccttg ctcaccattc ccactttgta caagaaagct
     2821 gggtacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
     2881 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
     2941 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
     3001 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg
     3061 ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc
     3121 ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc gatgcggttc
     3181 accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg
     3241 aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc
     3301 tgcttcatgt ggtcggggta gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg
     3361 agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg
     3421 ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc gtttacgtcg
     3481 ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc
     3541 ataggttgag aagatggctc tattttcatg cgtttaagag aaggctgcat ggagcctgct
     3601 tttttgtaca aacttgctga tatcactagt gcggccgcct gcaggtcgac tagaatagta
     3661 aattgtaatg ttgtttgttg tttgttttgt tgtggtaatt gttgtaaaaa taccggagtc
     3721 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
     3781 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     3841 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     3901 ctgtcggcag gggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
     3961 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
     4021 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
     4081 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacg
     4141 aagattttct tcttgtcatt gagtcgtaaa agactctgta tgaactgttc gccagtcttc
     4201 acggcgagtt ctgttagatc ctcgatctga atttttgact ccatggcctt tgattcagta
     4261 ggaactactt tcttagagac tccaatctct attacttgcc ttggtttatg aagcaagcct
     4321 tgaatcgtcc atactggaat agtacttctg atcttgagaa atatatcttt ctctgtgttc
     4381 ttgatgcagt tagtcctgaa tcttttgact gcatctttaa ccttcttggg aaggtatttg
     4441 atctcctgga gattattact cgggtagatc gtcttgatga gacctgccgc gtaggcctct
     4501 ctaaccatct gtgggtcagc attctttctg aaattgaaga ggctaatctt ctcattatcg
     4561 gtggtgaaca tggtatcgtc accttctccg tcgaactttc ttcctagatc gtagagatag
     4621 agaaagtcgt ccatggtgat ctccggggca aaggagatca gcttggctct agtcgaccat
     4681 atgggagagc tcccaacgcg ttggatgcat agcttgagta ttctatagtg tcacctaaat
     4741 gacaactttt ctatacaaag ttgatagctt ggcgtaatca tggtcatagc tgtttcctgt
     4801 gtgaaattgt tatccgctcg ggcccaagct tagcttgagc ttggatcaga ttgtcgtttc
     4861 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     4921 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     4981 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttaaagta
     5041 ctttaaagta ctttaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     5101 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     5161 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     5221 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     5281 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     5341 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     5401 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     5461 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     5521 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     5581 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     5641 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     5701 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     5761 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     5821 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     5881 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     5941 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     6001 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     6061 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     6121 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     6181 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     6241 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     6301 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     6361 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     6421 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     6481 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     6541 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     6601 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     6661 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     6721 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     6781 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     6841 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     6901 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     6961 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     7021 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     7081 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     7141 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     7201 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     7261 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc
     7321 cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc
     7381 gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc
     7441 ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat
     7501 taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt
     7561 gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga
     7621 ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc
     7681 agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca
     7741 tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt
     7801 ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa
     7861 agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac
     7921 gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg
     7981 ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga
     8041 ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga
     8101 ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc
     8161 aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg
     8221 agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga
     8281 gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa
     8341 cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat
     8401 tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg
     8461 gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat
     8521 tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc
     8581 ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc
     8641 tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct
     8701 acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct
     8761 acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg
     8821 cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     8881 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8941 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     9001 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     9061 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     9121 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9181 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9241 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9481 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9661 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9721 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     9781 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9841 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9901 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9961 ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat
    10021 aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg
    10081 cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaatttctag ctagacatta
    10141 tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
    10201 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
    10261 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
    10321 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
    10381 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
    10441 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
    10501 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
    10561 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
    10621 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
    10681 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
    10741 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
    10801 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
    10861 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
    10921 ttcccccatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
    10981 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg
    11041 catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
    11101 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
    11161 ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgcccgaa t
//
