LOCUS       pKNGSTAP               14132 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...1yg-dx3gud8
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|482233282|                                                 
            VNTDBDATE|482234797|                                               
            LSOWNER|                                                           
            VNTNAME|pKNGSTAP|                                                  
            VNTAUTHORNAME|Mansour Karimi|                                      
            VNTAUTHORTEL|003293313838|                                         
            VNTAUTHOREML|mansour.karimi@psb.ugent.be|                          
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FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="11207741313453995c8fc02e-ac23-42d2-a662
                     -2a172c7a0dd0"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     complement(1294..1313)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.6"
                     /annotation_group="11207773370548998696c271-b374-4076-8134
                     -140ac4e17e8f"
                     /created_by="primer3"
                     /Sequence="CGCCCGGTTCTTTTTGTCAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P157"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1728..1732
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1748
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1750^1751
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     primer          1783..1803
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     primer_bind     complement(1808..1828)
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.5"
                     /annotation_group="112077821557360095453fd0-c3dd-4a8e-808e
                     -aa0ded0a7771"
                     /created_by="primer3"
                     /Sequence="ACAAAGTTGATAGCTTGGCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P165"
     attR4           1820..1943
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(1984..2289)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(2290..2608)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.06%"
                     /Primary_Match="99.06%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2363
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2442
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(2616..2636)
                     /%GC="57.1"
                     /Hairpin_Tm="33.7"
                     /Self_Dimer_Tm="6.0"
                     /Tm="60.1"
                     /annotation_group="1120778326357899efccd1c5-73d3-47bb-af93
                     -a0ae599a037b"
                     /created_by="primer3"
                     /Sequence="GCGTAATCTAGAGGATCCGGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P166"
     misc_feature    complement(2631..3289)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    2640^2641
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     promoter        complement(3343..3373)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    3381^3382
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR3           complement(3397..3521)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     primer_bind     3569..3593
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /Sequence="ACAACATTACAATTACAATGGGCAC"
                     /Tm="58.8"
                     /%GC="36.0"
                     /Hairpin_Tm="36.6"
                     /Self_Dimer_Tm="13.1"
                     /modified_by="mamee"
                     /label="P173"
     CDS             3586..4104
                     /label="GStag"
     primer_bind     complement(3727..3746)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /Sequence="GCGTCATCGTAGGTCCAGAC"
                     /Tm="60.2"
                     /%GC="60.0"
                     /Hairpin_Tm="39.5"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P174"
     primer_bind     3966..3985
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /Sequence="GAACCTCTACTTCCAGGGCG"
                     /Tm="59.8"
                     /%GC="60.0"
                     /Hairpin_Tm="33.6"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P175"
     primer_bind     complement(4112..4131)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /Sequence="TGTGATCTCGAGTGAACCGC"
                     /Tm="60.1"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.1"
                     /modified_by="mamee"
                     /label="P176"
     attR1           4129..4253
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        4278..4308
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    4332
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4362..5021
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    4946
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     5024..5043
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.8"
                     /Tm="59.9"
                     /annotation_group="11207770064125008487f7c7-21e3-4fa5-ba1a
                     -726606568150"
                     /created_by="primer3"
                     /Sequence="GCGTGGATCCGGCTTACTAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P154"
     UTR             5044..5362
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    5136
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5354
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             5363..5668
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(5709..5833)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      5879..6104
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     6134..6153
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="16.7"
                     /Tm="60.0"
                     /annotation_group="6069050657713600b051da3e-bdfd-428a-b4b5
                     -6f349fd15497"
                     /created_by="primer3"
                     /Sequence="TTGCATGCCCCCACTACAAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P38"
     promoter        6135..6531
                     /modified_by="mamee"
                     /X-Transferred_From="pROLD"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dgycryx"">pROLD</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD 2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pROLD
                     (<a href=""urn:local:.:em-f1u7wbe"">pROLD 2</a>)"
                     /label="pROLD"
     misc_feature    6515..6522
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6647
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     CDS             6685..7482
                     /label="EgfpER"
     misc_feature    6712
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     6931..6950
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="112077557009080080b446b7-26aa-4f33-9de5
                     -421081fcc622"
                     /created_by="primer3"
                     /Sequence="CTCGTGACCACCCTGACCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P27"
     terminator      7511..7736
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="97.35%"
                     /Primary_Match="97.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     misc_feature    7603
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     T-DNA_border    7748..7948
                     /modified_by="mamee"
                     /label="RB"
     primer_bind     7769..7788
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="112077468446239953a656d4-e4a2-41d5-a172
                     -ce1407c3a737"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P14"
     misc_feature    7863
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    7956..7985
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     8597..8615
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="112077335107519921c86cb7-503d-4caa-87dc
                     -648c70aa726d"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P1"
     regulatory      9164..9793
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     9389..9408
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="11207734622410999c702b7a-d7cf-4d02-a1dc
                     -b149840bf346"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P2"
     primer_bind     10186..10204
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="112077357138739967c06ee7-c574-4062-9b48
                     -d424e9db76d4"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P3"
     regulatory      10222..11295
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    10226
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     10990..11007
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="112077368788030050f885c5-46c8-47cd-b7f2
                     -72a352397487"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P4"
     rep_origin      11361..11555
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     11790..11809
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1120773802168200e77b4222-9f5c-4b31-99c8
                     -28f77cd1b1eb"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P5"
     regulatory      11929..12189
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(12225..12813)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     12614..12633
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="1120773910653299cff47009-4266-4485-826a
                     -0184fb5bba09"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P6"
     misc_feature    12999^13000
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    13011^13012
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    13016..13019
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(13059..13850)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     13386..13405
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="1120774020988900a246f96b-e00f-4756-860d
                     -ea68899534a4"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNGSTAP"
                     /label="P7"
     Promoter        complement(13851..14125)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    13985^13986
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    13996^13997
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14064..14065
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    14068^14069
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14101^14102
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgcgg tacccgggga tcctctagag ggcccggcgc gcccaggaaa cagctatgac
     1801 catgattacg ccaagctatc aactttgtat agaaaagttg aacgagaaac gtaaaatgat
     1861 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     1921 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
     1981 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
     2041 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
     2101 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
     2161 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
     2221 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
     2281 aaactgcatt tcaccagccc ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc
     2341 gggcgacctc agccatccct tcttgatttt ccgctttcca gcgttcggca cgcagacgac
     2401 gggcttcatt ctgcatggtt gtgcttaccg aaccggagat actgacatca tatatgcctt
     2461 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
     2521 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     2581 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
     2641 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2701 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2761 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2821 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     2881 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2941 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     3001 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     3061 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     3121 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     3181 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     3241 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     3301 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
     3361 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
     3421 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     3481 atcattttac gtttctcgtt caactttatt atacatagtt gataattcac tggccgtcgt
     3541 tttacttaat taaaacaaca aacaacaaac aacattacaa ttacaatggg cacccccgca
     3601 gtcaccacct acaagctggt cattaacggc aagactctga agggcgagac caccaccaag
     3661 gccgtggacg cagaaaccgc ggaaaaggcg ttcaagcagt acgcgaacga caacggggtg
     3721 gacggagtct ggacctacga tgacgctaca aaaacgttca ccgtgaccga ggttaacacc
     3781 cccgctgtga ctacgtacaa gctggtgatc aatgggaaga ccctgaaggg cgagaccacc
     3841 acgaaagctg tagacgccga gacagccgag aaggccttca agcagtacgc caatgacaac
     3901 ggcgtggatg gcgtgtggac ctatgacgac gccacgaaga cattcactgt gactgagatc
     3961 gatgagaacc tctacttcca gggcggcagt atggacgaga agaccaccgg ctggagaggc
     4021 ggccacgtgg tcgagggact ggccggtgag ctggagcagc tacgggcccg ccttgagcac
     4081 cacccccagg ggcagcgcga gccgggtgga ggcggttcac tcgagatcac aagtttgtac
     4141 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     4201 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     4261 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     4321 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     4381 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     4441 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     4501 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     4561 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     4621 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     4681 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     4741 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     4801 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     4861 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     4921 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     4981 aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac
     5041 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5101 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5161 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     5221 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     5281 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     5341 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     5401 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     5461 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     5521 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     5581 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5641 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5701 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5761 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     5821 tgtacaaagt ggtgatatca ctagtgcggc cgcctgagct catctaactg actagccgcg
     5881 gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
     5941 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
     6001 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     6061 tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcagga agcttgcatg
     6121 cctgcaggtc agcttgcatg cccccactac aatgaatttg ttcgtgaact attagttgcg
     6181 ggccttggca tccgactacc tctgcggcaa tattatattc cctgggccca ccgtgaaccc
     6241 aatttcgcct atttattcat tacccccatt aacattgaag tagtcatgat gggcctgcag
     6301 cacgttggtg aggctggcac aactcatcca tatactttct gaccggatcg gcacattatt
     6361 gtagaaaacg cggacccaca gcgcactttc caaagcggtg ccgcgtcaga atgcgctggc
     6421 agaaaaaaat taatccaaaa gtaccctcca agcagcccat ataaacgcgt ttacaaatcc
     6481 gctaacctca acaatttgag cagagaaaat tcgcacctac aatagaggat ccccgggtac
     6541 cgagctcgaa ttctcaacac aacatataca aaacaaacga atctcaagca atcaagcatt
     6601 ctacttctat tgcagcaatt taaatcattt cttttaaagc aaaagcaatt ttctgaaaat
     6661 tttcaccatt tacgaacgat agccatggtg aagactaatc tttttctctt tctcatcttt
     6721 tcacttctcc tatcattatc ctcggccgac atggtgagca agggcgagga gctgttcacc
     6781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     6841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     6901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     6961 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     7021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7201 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     7261 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     7321 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     7381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     7441 actctcggca tggacgagct gtacaagaag gacgagctgt aagcgccgcc cggctgcagc
     7501 ccgggggatc cactagttct agagtccgca aaaatcacca gtctctctct acaaatctat
     7561 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     7621 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     7681 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag
     7741 gcatgcaagc ttagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     7801 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     7861 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     7921 caaccacagg gttcccctcg ggatcaaagt actttaaagt actttaaagt actttaaagt
     7981 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     8041 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     8101 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     8161 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     8221 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     8281 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8341 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8401 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8461 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     8521 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     8581 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     8641 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     8701 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     8761 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     8821 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     8881 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     8941 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
     9001 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     9061 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     9121 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     9181 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     9241 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9301 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9361 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9421 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9481 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     9541 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     9601 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     9661 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     9721 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     9781 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     9841 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     9901 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     9961 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
    10021 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
    10081 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
    10141 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
    10201 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
    10261 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
    10321 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
    10381 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
    10441 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
    10501 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
    10561 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
    10621 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
    10681 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
    10741 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
    10801 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
    10861 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
    10921 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
    10981 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
    11041 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
    11101 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
    11161 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
    11221 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
    11281 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
    11341 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
    11401 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
    11461 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
    11521 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
    11581 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
    11641 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
    11701 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
    11761 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11821 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11881 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
    11941 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    12001 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    12061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    12241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    12661 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    12721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    12781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca tgatatatct
    12901 cccaatttgt gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga
    12961 tagtttggct gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg
    13021 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
    13081 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
    13141 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
    13201 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
    13261 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
    13321 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
    13381 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
    13441 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
    13501 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
    13561 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
    13621 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
    13681 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
    13741 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
    13801 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
    13861 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
    13921 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
    13981 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
    14041 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa
    14101 caggcttatg tccactgggt tcgtgcccga at
//
