LOCUS       pXK7S*NF2              12307 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...sl-dozjzn3
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459374645|                                                 
            VNTDBDATE|459374645|                                               
            LSOWNER|                                                           
            VNTNAME|pXK7S*NF2|                                                 
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
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                               "{\\i Sac}I (8582)" "@N (@S)" 5 -14.5568        
                                   -1.15807 0 -10 2.97739 0.704328 #357#))     
                               (CObjectList))                                  
                         #359=(CGroupWidget                                    
                               (CWidget 2 (10 #19# 0) 1 2 0 0 Nil -1489 100)   
                                 (CObjectList                                  
                                  #360=(CScratch                               
                                        (CWidget 1 (1 #19# 1) 1 2 0 0 #284#
            39428488                                 100) (LOGPEN 0 0 10079283)
            1 1 6.23438 1.9                                0.123113 1)         
                                                     #361=(CLabel              
                                                           (CWidget 0 (0 0) 1 2
            0 0 #263# 14352960 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 43
            16 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Sac}II (77)" "@N (@S)" 5 2.86517                             
              13.8968 0 -10 2.52918 0.704328 #360#)                         
            #362=(CScratch                                                     
                  (CWidget 2 (1 #19# 2) 1 2 0 0 #284# 39303716                 
                           100) (LOGPEN 0 0 10079283) 1 1 6.03678 1.9          
                                 0.123113 1)                                   
                           #363=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #263# 14352960 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sac}II (464)" "@N (@S)" 5
            11.1104                                9.26456 0 -10 2.81731
            0.704328 #362#))                         (CObjectList))            
                                               #364=(CGroupWidget              
                                                     (CWidget 2 (10 #20# 0) 1 2
            0 0 Nil -693 100)                         (CObjectList             
                                                       #365=(CScratch          
                                                             (CWidget 1 (1 #20#
            1) 1 2 0 0 #284# 28342192                                 100)
            (LOGPEN 0 0 10079283) 1 1 6.10724 1.9                              
             0.123113 1)                                                  
            #366=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #263# 14352960 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Sal}I (326)" "@N (@S)" 5 7.85619          
                                 11.5807 0 -10 2.52918 0.704328 #365#)         
                            #367=(CScratch                                     
                                  (CWidget 2 (1 #20# 2) 1 2 0 0 #284# 28498340 
                                           100) (LOGPEN 0 0 10079283) 1 1
            1.3638 1.9                                0.123113 1)              
                                                #368=(CLabel                   
                                                      (CWidget 0 (0 0) 1 2 0 0
            #263# 14352976 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 43
            16 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Sal}I (9616)" "@N (@S)" 5 -14.3355                           
                2.31614 0 -10 2.83332 0.704328 #367#))                        
            (CObjectList))                                               
            #369=(CGroupWidget                                                 
                  (CWidget 2 (10 #23# 0) 1 2 0 0 Nil -983 100)                 
                   (CObjectList                                                
                    #370=(CScratch                                             
                          (CWidget 1 (1 #23# 1) 1 2 0 0 #284# 39421080         
                                   100) (LOGPEN 0 0 10079283) 1 1 5.38424 1.9  
                                         0.123113 1)                           
                                   #371=(CLabel (CWidget 0 (0 0) 1 2 0 0 #263#
            -1 100)                                (LOGPEN 0 0 8437824) 1      
                                                   (LOGFONT 43 16 0 0 400 255 0
            0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sph}I
            (1742)" "@N (@S)" 5 12.8781                                6.94842
            0 -10 2.97739 0.704328 #370#)                         
            #372=(CScratch                                                     
                  (CWidget 2 (1 #23# 2) 1 2 0 0 #284# 29486792                 
                           100) (LOGPEN 0 0 10079283) 1 1 2.04698 1.9          
                                 0.123113 1)                                   
                           #373=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #263# 28844756 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sph}I (8278)" "@N (@S)" 5
            -13.5468                                -8.10649 0 -10 2.97739
            0.704328 #372#))                         (CObjectList)))
            (CObjectList))                         #374=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -1271 100)                  (CObjectList)
            (CObjectList))                                  #375=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -549 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 70   
            (CStringList) 21815400 95 (CObList))).
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="605562047794050072b8077e-d402-4c0e-ab69
                     -810eae998fb2"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     771..790
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="6055620769558800c4e71fe1-4011-4634-8ff8
                     -5d3bfd84a536"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P24"
     primer_bind     1384..1403
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="6055620829569100395c6392-0162-4e17-9242
                     -231911947d19"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P25"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1725..1729
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     terminator      complement(1772..1997)
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     misc_feature    1989^1990
                     /Original_Bases="CATGGCCGCTCCCGGCCGC"
                     /label="CATGGCCGCTCCCGGCCGC"
                     /note="Geneious type: Editing History Deletion"
     primer          complement(2036..2051)
                     /label="M13F"
     CDS             complement(2082..3893)
                     /label="GUS"
     primer_bind     complement(2588..2607)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="14.5"
                     /Tm="60.2"
                     /annotation_group="6055621062332300fb4ff6fe-7778-46dc-a166
                     -1ed2fb8f2425"
                     /created_by="primer3"
                     /Sequence="GGAAGCAACGCGTAAACTCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P32"
     primer_bind     complement(3176..3195)
                     /%GC="50.0"
                     /Hairpin_Tm="46.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="60556210017839002103fbef-64ea-4049-9ce1
                     -098574e8f615"
                     /created_by="primer3"
                     /Sequence="TTTGCAAGTGGTGAATCCGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P31"
     primer_bind     complement(3785..3804)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="6055620943704900cad9c34b-9a4b-48a7-9af4
                     -8f10463a5463"
                     /created_by="primer3"
                     /Sequence="TGATCAGCGTTGGTGGGAAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P30"
     CDS             complement(3921..4637)
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="99.16%"
                     /Primary_Match="99.16%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8y-dgycryy"">GFP</a>"
                     /label="GFP"
     primer_bind     complement(4438..4457)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="6055620886638000729087e9-4c7d-42f8-a04b
                     -0157fac2ecb2"
                     /created_by="primer3"
                     /Sequence="CTCGTGACCACCCTGACCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P27"
     NLS             complement(4638..4690)
                     /modified_by="mamee"
                     /label="c2 NLS"
     misc_feature    4712
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        complement(4741..5787)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     misc_feature    4807^4808
                     /Original_Bases="CGGATCGTC"
                     /label="CGGATCGTC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    4809
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4811
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5006
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(5099..5118)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.1"
                     /Tm="60.2"
                     /annotation_group="6055620595545300a31c8847-f262-423a-a719
                     -a1e52255b556"
                     /created_by="primer3"
                     /Sequence="CGGATTCCATTGCCCAGCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P11"
     primer_bind     complement(5584..5604)
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="60.0"
                     /annotation_group="605562053658880067bb6650-c675-4ce5-add9
                     -6d6fe6160242"
                     /created_by="primer3"
                     /Sequence="GATGGTTAGAGAGGCCTACGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P10"
     misc_feature    5755..5767
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5836..5837
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          complement(5878..5894)
                     /label="M13 R"
     T-DNA_border    5922..6152
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     5943..5962
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6055620653276100b58816cf-30a0-4b9a-8e9b
                     -e48b2e85b2ee"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P14"
     misc_feature    6037
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6120..6149
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(6205..6224)
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="6055621247573200e867aa2e-59e4-432d-9882
                     -ca5e59585122"
                     /created_by="primer3"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P37"
     primer_bind     6771..6789
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="6055620067070200f216c35c-cfd2-4423-8888
                     -1761ce651fc4"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P1"
     regulatory      7338..7967
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     7563..7582
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6055620130150300455d86cf-43a8-49b3-a222
                     -a0da3b3e3b3d"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P2"
     primer_bind     8360..8378
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="6055620188345400d24a0586-67e1-4f6a-a783
                     -2d090e2e24f0"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P3"
     regulatory      8396..9469
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    8400
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     9164..9181
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="60556202486213005b77ec9a-f378-4adb-aa1f
                     -29223292c530"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P4"
     rep_origin      9535..9729
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     9964..9983
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60556203054246004ccd73c9-1ded-44d1-99cc
                     -5acdd1b53fb0"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P5"
     regulatory      10103..10363
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(10399..10987)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     10788..10807
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="60556203626386007c8e1ec7-6637-4e47-ad2a
                     -fe78868d211c"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P6"
     misc_feature    11186^11187
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11191..11194
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(11234..12025)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     11561..11580
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="6055620419827800dd3c8c39-2b60-4285-ae45
                     -36c99f2c949a"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pXK7S*NF2"
                     /label="P7"
     Promoter        complement(12026..12300)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    12160^12161
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    12168^12169
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    12239..12240
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    12243^12244
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    12276^12277
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgagc tctcccatat ggtcgacctg caggtcactg gattttggtt ttaggaatta
     1801 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
     1861 ctcaacacat gagcgaaacc ctataagaac cctaattccc ttatctggga actactcaca
     1921 cattattctg gagaaaaata gagagagata gatttgtaga gagagactgg tgatttttgc
     1981 ggactctagc atggccgcgg ctagtcagtt agatcgtttt cccagtcacg acgttgtaaa
     2041 acgacggcca gtgaattatc aactatgtat aataaagttg ttcattgttt gcctccctgc
     2101 tgcggttttt caccgaagtt catgccagtc cagcgttttt gcagcagaaa agccgccgac
     2161 ttcggtttgc ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct
     2221 tgcgaggtcg caaaatcggc gaaattccat acctgttcac cgacgacggc gctgacgcga
     2281 tcaaagacgc ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg
     2341 gtgtacattg agtgcagccc ggctaacgta tccacgccgt attcggtgat gataatcggc
     2401 tgatgcagtt tctcctgcca ggccagaagt tctttttcca gtaccttctc tgccgtttcc
     2461 aaatcgccgc tttggacata ccatccgtaa taacggttca ggcacagcac atcaaagaga
     2521 tcgctgatgg tatcggtgtg agcgtcgcag aacattacat tgacgcaggt gatcggacgc
     2581 gtcgggtcga gtttacgcgt tgcttccgcc agtggcgcga aatattcccg tgcaccttgc
     2641 ggacgggtat ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca
     2701 cgcgctatca gctctttaat cgcctgtaag tgcgcttgct gagtttcccc gttgactgcc
     2761 tcttcgctgt acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg
     2821 ttaaagccga cagcagcagt ttcatcaatc accacgatgc catgttcatc tgcccagtcg
     2881 agcatctctt cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc
     2941 attaatgcgt ggtcgtgcac catcagcacg ttatcgaatc ctttgccacg caagtccgca
     3001 tcttcatgac gaccaaagcc agtaaagtag aacggtttgt ggttaatcag gaactgttcg
     3061 cccttcactg ccactgaccg gatgccgacg cgaagcgggt agatatcaca ctctgtctgg
     3121 cttttggctg tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga
     3181 ttcaccactt gcaaagtccc gctagtgcct tgtccagttg caaccacctg ttgatccgca
     3241 tcacgcagtt caacgctgac atcaccattg gccaccacct gccagtcaac agacgcgtgg
     3301 ttacagtctt gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg
     3361 gtgtagagca ttacgctgcg atggattccg gcatagttaa agaaatcatg gaagtaagac
     3421 tgctttttct tgccgttttc gtcggtaatc accattcccg gcgggatagt ctgccagttc
     3481 agttcgttgt tcacacaaac ggtgatacgt acacttttcc cggcaataac atacggcgtg
     3541 acatcggctt caaatggcgt atagccgccc tgatgctcca tcacttcctg attattgacc
     3601 cacactttgc cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa
     3661 cctttcggta taaagacttc gcgctgatac cagacgttgc ccgcataatt acgaatatct
     3721 gcatcggcga actgatcgtt aaaactgcct ggcacagcaa ttgcccggct ttcttgtaac
     3781 gcgctttccc accaacgctg atcaattcca cagttttcgc gatccagact gaatgcccac
     3841 aggccgtcga gttttttgat ttcacgggtt ggggtttcta caggacgtaa cattcccact
     3901 ttgtacaaga aagctgggta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     3961 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     4021 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     4081 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     4141 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     4201 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     4261 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     4321 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     4381 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag
     4441 gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     4501 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     4561 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     4621 tcgcccttgc tcaccatagg ttgagaagat ggctctattt tcatgcgttt aagagaaggc
     4681 tgcatggagc ctgctttttt gtacaaactt gctgatatca ctagtgcggc cgcctgcagg
     4741 tcgactagaa tagtaaattg taatgttgtt tgttgtttgt tttgttgtgg taattgttgt
     4801 aaaaataccg gagtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
     4861 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
     4921 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
     4981 ccatctttgg gaccactgtc ggcaggggca tcttgaacga tagcctttcc tttatcgcaa
     5041 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
     5101 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
     5161 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
     5221 tccaccatgt tgacgaagat tttcttcttg tcattgagtc gtaaaagact ctgtatgaac
     5281 tgttcgccag tcttcacggc gagttctgtt agatcctcga tctgaatttt tgactccatg
     5341 gcctttgatt cagtaggaac tactttctta gagactccaa tctctattac ttgccttggt
     5401 ttatgaagca agccttgaat cgtccatact ggaatagtac ttctgatctt gagaaatata
     5461 tctttctctg tgttcttgat gcagttagtc ctgaatcttt tgactgcatc tttaaccttc
     5521 ttgggaaggt atttgatctc ctggagatta ttactcgggt agatcgtctt gatgagacct
     5581 gccgcgtagg cctctctaac catctgtggg tcagcattct ttctgaaatt gaagaggcta
     5641 atcttctcat tatcggtggt gaacatggta tcgtcacctt ctccgtcgaa ctttcttcct
     5701 agatcgtaga gatagagaaa gtcgtccatg gtgatctccg gggcaaagga gatcagcttg
     5761 gctctagtcg accatatggg agagctccca acgcgttgga tgcatagctt gagtattcta
     5821 tagtgtcacc taaatgacaa cttttctata caaagttgat agcttggcgt aatcatggtc
     5881 atagctgttt cctgtgtgaa attgttatcc gctcgggccc aagcttagct tgagcttgga
     5941 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
     6001 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     6061 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     6121 aagtacttta aagtacttta aagtacttta aagtactttg atccaacccc tccgctgcta
     6181 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     6241 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     6301 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     6361 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     6421 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     6481 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     6541 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     6601 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     6661 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     6721 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     6781 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     6841 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     6901 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     6961 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     7021 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     7081 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     7141 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     7201 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     7261 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     7321 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     7381 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     7441 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     7501 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     7561 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     7621 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     7681 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     7741 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     7801 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     7861 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     7921 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     7981 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     8041 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     8101 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     8161 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     8221 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     8281 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     8341 gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     8401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     8461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     8521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     8581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     8641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     8701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     8761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     8821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     8881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     8941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     9001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     9061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     9121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     9181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     9241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     9301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     9361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     9421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     9481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     9541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     9601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     9661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     9721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     9781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     9841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     9901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     9961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    10021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
    10081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
    10141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    10201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    10861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11041 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
    11101 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
    11161 ttagtgcatc taatcgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat
    11221 ttctagctag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
    11281 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
    11341 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
    11401 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
    11461 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
    11521 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
    11581 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
    11641 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
    11701 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg
    11761 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
    11821 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
    11881 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
    11941 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
    12001 cttcggcgat caccgcttcc cccatgatgt ttaactttgt tttagggcga ctgccctgct
    12061 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
    12121 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
    12181 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
    12241 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
    12301 cccgaat
//
