LOCUS       pEN-L4-UAS-R1           2836 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...20-dt8d430
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459276709|                                                 
            VNTDBDATE|459276709|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L4-UAS-R1,0|                                           
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                                  #234=(CLabel                                 
                                        (CWidget 0 (0 0) 1 2 0 0 #158# 327728
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 39 14 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xba}I
            (162)" "@N (@S)" 5 7.8504 11.5807                                0
            -10 2.61515 0.711604 #233#)) (CObjectList))                  
            #235=(CGroupWidget                                                 
                  (CWidget 2 (10 #13# 0) 1 2 0 0 Nil -305 100)                 
                   (CObjectList                                                
                    #236=(CScratch                                             
                          (CWidget 1 (1 #13# 1) 1 2 0 0 #178# 0 100)           
                                (LOGPEN 0 0 10079283) 1 1 5.88961 1.9          
                                 0.123113 1)                                   
                           #237=(CLabel (CWidget 0 (0 0) 1 2 0 0 #157# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Pst}I (173)" "@N (@S)" 5
            10.8382                                9.26456 0 -10 2.50841
            0.711604 #236#)                          #238=(CScratch            
                                                           (CWidget 2 (1 #13#
            2) 1 2 0 0 #178# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 0.127114 1.9                                0.123113
            1)                                                   #239=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #157# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Pst}I (2757)" "@N (@S)" 5 -4.05088                     
                      16.213 0 -10 2.81084 0.711604 #238#))                    
                (CObjectList))                                               
            #240=(CGroupWidget                                                 
                  (CWidget 2 (10 #14# 0) 1 2 0 0 Nil -1086 100)                
                    (CObjectList                                               
                     #241=(CScratch                                            
                           (CWidget 1 (1 #14# 1) 1 2 0 0 #178# 0 100)          
                                 (LOGPEN 0 0 10079283) 1 1 5.91414 1.9         
                                  0.123113 1)                                  
                            #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #157# 0 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Sac}I (162)" "@N (@S)" 5
            9.64944                                10.4226 0 -10 2.61515
            0.711604 #241#)                          #243=(CScratch            
                                                           (CWidget 2 (1 #14#
            2) 1 2 0 0 #178# 28808408                                 100)
            (LOGPEN 0 0 10079283) 1 1 0.613269 1.9                             
              0.123113 1)                                                  
            #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #157# 0 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Sac}I (2539)" "@N (@S)" 5 -9.65656        
                                   11.5807 0 -10 2.91758 0.711604 #243#))      
                              (CObjectList))) (CObjectList))                   
                 #245=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1061 100)  
                           (CObjectList) (CObjectList))                        
                     #246=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -994 100)
                             (CObjectList) (CObjectList))) (CObjectList)))     
               (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
            50855937    (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     attL4           1..97
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
     misc_feature    98..120
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_binding    121..290
                     /label="UAS"
     attR1           291..447
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="99.36%"
                     /Primary_Match="99.36%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:y-f0o11e8"">attR1</a>"
     misc_feature    417
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer          complement(507..527)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        588..638
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    639..1448
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     664..683
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="60550967382869009a8db9d2-2c73-4ba0-84b5
                     -a037b5a47b27"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UAS-R1"
                     /label="P44"
     primer_bind     1298..1317
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="605509662293570059618f39-014e-4ffd-82ca
                     -f232a0425ebb"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UAS-R1"
                     /label="P42"
     rep_origin      1595..2183
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    1742
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1987..1988
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2083..2102
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="60550967969236001929b3ae-3cc6-43d8-9bb5
                     -fcc88d12ace8"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UAS-R1"
                     /label="P45"
     primer_bind     2691..2710
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6055096854769400c029134b-69c5-42fd-9775
                     -2247d54af4d5"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UAS-R1"
                     /label="P46"
     primer          2782..2802
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
     misc_feature    2815
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttggaa ttcgatatga agcttgatat
      121 cgggtgacag ccctccgagc gggtgacagc cctccgaagc gggtgacagc cctccgacgt
      181 cgggtgacag ccctccgagc gggtgacagc cctccgagcg ggtgacagcc ctccgctgca
      241 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag caagtttgta
      301 caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      361 gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa
      421 ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag ggcggccgcg
      481 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      541 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      601 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      661 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      721 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
      781 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
      841 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
      901 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
      961 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1021 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1081 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1141 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1201 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1261 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1321 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1381 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1441 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     1501 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     1561 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1621 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1681 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1741 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1801 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1861 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1921 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1981 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2041 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2101 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2161 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2221 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2281 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2341 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2401 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2461 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     2521 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     2581 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     2641 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     2701 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     2761 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccgagttaa
     2821 cgctaccatg gagctc
//
