LOCUS       pEN-L4-pOp6M2-R1        3159 bp    DNA     circular     07-MAR-2023
DEFINITION  .
ACCESSION   urn.local...9b-dv3kw62
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459276655|                                                 
            VNTDBDATE|459276655|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L4-pOp6-M2-R1,0|                                       
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                   (CWidget 1 (10 #9# 0) 1 2 0 0 Nil -631 100)                 
                   (CObjectList                                                
                    #238=(CScratch                                             
                          (CWidget 1 (1 #9# 1) 1 2 0 0 #189# 0 100)            
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                                        (LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
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                                              "{\\i Nco}I (2530)" "@N (@S)" 5
            -14.0504                                4.63228 0 -10 2.91758
            0.711604 #238#))                         (CObjectList))            
                                               #240=(CGroupWidget              
                                                     (CWidget 1 (10 #10# 0) 1 2
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            (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
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                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Nde}I (103)" "@N (@S)" 5 4.94798                       
                    13.8968 0 -10 2.61515 0.711604 #241#))                     
               (CObjectList))                                               
            #243=(CGroupWidget                                                 
                  (CWidget 1 (10 #12# 0) 1 2 0 0 Nil -956 100)                 
                   (CObjectList                                                
                    #244=(CScratch                                             
                          (CWidget 1 (1 #12# 1) 1 2 0 0 #189# 0 100)           
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                           #245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Nru}I (425)" "@N (@S)" 5
            12.577 6.94842                                0 -10 2.50841
            0.711604 #244#)) (CObjectList))                  
            #246=(CGroupWidget                                                 
                  (CWidget 1 (10 #15# 0) 1 2 0 0 Nil -240 100)                 
                   (CObjectList                                                
                    #247=(CScratch                                             
                          (CWidget 1 (1 #15# 1) 1 2 0 0 #189# 0 100)           
                                (LOGPEN 0 0 10079283) 1 1 1.01021 1.9          
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                           #248=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)  
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                                         (LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1
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                                              "{\\i Sac}II (2658)" "@N (@S)" 5
            -13.0678                                9.26456 0 -10 3.09548
            0.711604 #247#))                         (CObjectList))            
                                               #249=(CGroupWidget              
                                                     (CWidget 1 (10 #16# 0) 1 2
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            1) 1 2 0 0 #189# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 0.0257504 1.9                                0.123113
            1)                                                   #251=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #169# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 1 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Sal}I (3155)" "@N (@S)" 5 -2.7766                      
                     17.3711 0 -10 2.73968 0.711604 #250#))                    
                (CObjectList))                                               
            #252=(CGroupWidget                                                 
                  (CWidget 1 (10 #18# 0) 1 2 0 0 Nil -241 100)                 
                   (CObjectList                                                
                    #253=(CScratch                                             
                          (CWidget 1 (1 #18# 1) 1 2 0 0 #189# 0 100)           
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                                                "{\\i Spe}I (2987)" "@N (@S)" 5
            -6.82965                                12.7388 0 -10 2.91758
            0.711604 #253#))                         (CObjectList))            
                                               #255=(CGroupWidget              
                                                     (CWidget 2 (10 #11# 0) 1 2
            0 0 Nil -305 100)                         (CObjectList             
                                                       #256=(CScratch          
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            1)                                                   #257=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Not}I (177)" "@N (@S)" 5 10.8614                       
                    9.26456 0 -10 2.50841 0.711604 #256#)                      
               #258=(CScratch                                                  
                     (CWidget 2 (1 #11# 2) 1 2 0 0 #189# 0 100)                
                           (LOGPEN 0 0 10079283) 1 1 1.03398 1.9               
                            0.123113 1)                                        
                      #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)       
                                    (LOGPEN 0 0 8437824) 1                     
                                    (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34  
                                          "Arial") 1.15807 0.611111 0          
                                         "{\\i Not}I (2646)" "@N (@S)" 5
            -13.0036                                8.10649 0 -10 2.81084
            0.711604 #258#))                         (CObjectList))            
                                               #260=(CGroupWidget              
                                                     (CWidget 2 (10 #13# 0) 1 2
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                                                       #261=(CScratch          
                                                             (CWidget 1 (1 #13#
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            (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Pst}I (173)" "@N (@S)" 5 9.56838                       
                    10.4226 0 -10 2.50841 0.711604 #261#)                      
               #263=(CScratch                                                  
                     (CWidget 2 (1 #13# 2) 1 2 0 0 #189# 0 100)                
                           (LOGPEN 0 0 10079283) 1 1 1.03794 1.9               
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                                         "{\\i Pst}I (2644)" "@N (@S)" 5
            -12.9178                                6.94842 0 -10 2.81084
            0.711604 #263#))                         (CObjectList))            
                                               #265=(CGroupWidget              
                                                     (CWidget 2 (10 #20# 0) 1 2
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            (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Xba}I (162)" "@N (@S)" 5 7.8504 11.5807                
                           0 -10 2.61515 0.711604 #266#)                       
                     #268=(CScratch                                            
                           (CWidget 2 (1 #20# 2) 1 2 0 0 #189# 0 100)          
                                 (LOGPEN 0 0 10079283) 1 1 0.976533 1.9        
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                                   (CWidget 0 (0 0) 1 2 0 0 #168# 4129328 100) 
                                          (LOGPEN 0 0 8437824) 1               
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                                                "{\\i Xba}I (2675)" "@N (@S)" 5
            -12.8458                                10.4226 0 -10 2.91758
            0.711604 #268#))                         (CObjectList)))
            (CObjectList))                         #270=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -1021 100)                  (CObjectList)
            (CObjectList))                                  #271=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -436 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 39611988   
            (CStringList) 39612048 39612024 (CObList))).
FEATURES             Location/Qualifiers
     attL4           1..97
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
     misc_feature    98^99
                     /Original_Bases="ACTGCAG"
                     /label="ACTGCAG"
                     /note="Geneious type: Editing History Deletion"
     misc_binding    133..444
                     /label="6XOP"
     primer_bind     456..475
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-pOp6M2-R1"
                     /Sequence="TCGCAAGACCCTTCCTCTAT"
                     /Tm="57.8"
                     /%GC="50.0"
                     /Hairpin_Tm="45.0"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P64"
     promoter        480..595
                     /modified_by="mamee"
                     /label="Minimal p35S+Omega"
     attR1           614..770
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="99.36%"
                     /Primary_Match="99.36%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:y-f0o11e8"">attR1</a>"
     misc_feature    613^614
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    740
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer          complement(833..849)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     promoter        911..961
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    962..1771
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     987..1006
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="60549301025611001f31788a-17c7-481e-8fd6
                     -24fecd82c469"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-pOp6M2-R1"
                     /label="P44"
     primer_bind     1621..1640
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60549299853427000fe6af08-9234-49da-9cd8
                     -5aed4bf1144d"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-pOp6M2-R1"
                     /label="P42"
     rep_origin      1918..2506
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.49%"
                     /Primary_Match="99.49%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    2065
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2310..2311
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2406..2425
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="60549301603514005bc2d7e3-5b87-4b58-b691
                     -bcec59b2fac3"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-pOp6M2-R1"
                     /label="P45"
     primer_bind     3014..3033
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6054930218666200c7761d67-5ed5-44f3-ad63
                     -67e69b5df9b1"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-pOp6M2-R1"
                     /label="P46"
     primer          3085..3101
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3105..3120
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     misc_feature    3138
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttggcg gccgccaccg cggtggagct
      121 caagaaatct agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga
      181 ctagaaagaa gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag
      241 aagaaaggga agagaaagaa ttgtgagcgc tcacaattga aagactagaa agaagaaagg
      301 gaagagaaag aattgtgagc gctcacaatt gaaagactag aaagaagaaa gggaagagaa
      361 agaattgtga gcgctcacaa ttgaaagact agaaagaaga aagggaagag aaagaattgt
      421 gagcgctcac aattgaaaga ctagtggatc gatcttcgca agacccttcc tctatataag
      481 gaagttcatt tcatttggag aggacacgct gaagctagac gcgcgtattt ttacaacaat
      541 taccaacaac aacaacaaac aacaacaaca ttacatttta cattctacaa ctacagctag
      601 caagcttgtc gaccaagttt gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat
      661 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      721 catatgcagt cactatgaac caactactta gatggtatta gtgacctgta gaattcgagc
      781 tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc
      841 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      901 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
      961 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1021 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1081 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1141 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1201 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1261 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1321 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1381 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1441 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1501 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1561 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1621 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1681 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1741 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     1801 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     1861 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2041 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2761 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
     2821 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     2881 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     2941 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3001 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac
     3061 tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag
     3121 tcttaagctc gggcccgagt taacgctacc atggagctc
//
