LOCUS       pEN-L1-LHOATG4-L2        3894 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...16a-dt4tpab
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459280024|                                                 
            VNTDBDATE|459280024|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L1-LHOATG4-L2,0|                                       
            VNTAUTHORNAME|Demo User|                                           
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                  (CWidget 1 (10 #18# 0) 1 2 0 0 Nil -867 100)                 
                   (CObjectList                                                
                    #237=(CScratch                                             
                          (CWidget 1 (1 #18# 1) 1 2 0 0 #189# 4129320          
                                  100) (LOGPEN 0 0 10079283) 1 1 0.600898 1.9  
                                         0.123113 1)                           
                                   #238=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169#
            0 100)                                (LOGPEN 0 0 8437824) 1       
                                                  (LOGFONT 39 14 0 0 400 255 1
            0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Spe}I
            (3522)" "@N (@S)" 5 -11.0756                                9.26456
            0 -10 2.91758 0.711604 #237#))                        
            (CObjectList))                                               
            #239=(CGroupWidget                                                 
                  (CWidget 1 (10 #20# 0) 1 2 0 0 Nil 4129208 100)              
                      (CObjectList                                             
                       #240=(CScratch                                          
                             (CWidget 1 (1 #20# 1) 1 2 0 0 #189# 327683        
                                    100) (LOGPEN 0 0 10079283) 1 1 2.08945 1.9 
                                          0.123113 1)                          
                                    #241=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169#
            0 100)                                (LOGPEN 0 0 8437824) 1       
                                                  (LOGFONT 39 14 0 0 400 255 1
            0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xba}I
            (2598)" "@N (@S)" 5 -13.3462                               
            -5.79035 0 -10 2.91758 0.711604 #240#))                        
            (CObjectList))                                               
            #242=(CGroupWidget                                                 
                  (CWidget 1 (10 #21# 0) 1 2 0 0 Nil 2 100)                    
                  (CObjectList                                                 
                   #243=(CScratch                                              
                         (CWidget 1 (1 #21# 1) 1 2 0 0 #189# 327695            
                                100) (LOGPEN 0 0 10079283) 1 1 1.85908 1.9     
                                      0.123113 1)                              
                                #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169# 0
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 39 14 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xho}I
            (2741)" "@N (@S)" 5 -14.324                                2.31614
            0 -10 2.91758 0.711604 #243#))                        
            (CObjectList))                                               
            #245=(CGroupWidget                                                 
                  (CWidget 1 (10 #22# 0) 1 2 0 0 Nil -986 100)                 
                   (CObjectList                                                
                    #246=(CScratch                                             
                          (CWidget 1 (1 #22# 1) 1 2 0 0 #189# 29277124         
                                   100) (LOGPEN 0 0 10079283) 1 1 2.02501 1.9  
                                         0.123113 1)                           
                                   #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #169#
            0 100)                                (LOGPEN 0 0 8437824) 1       
                                                  (LOGFONT 39 14 0 0 400 255 1
            0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xma}I
            (2638)" "@N (@S)" 5 -13.8751                               
            -4.63228 0 -10 2.98874 0.711604 #246#))                        
            (CObjectList))                                               
            #248=(CGroupWidget                                                 
                  (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -630 100)                  
                  (CObjectList                                                 
                   #249=(CScratch                                              
                         (CWidget 1 (1 #1# 1) 1 2 0 0 #189# 0 100)             
                              (LOGPEN 0 0 10079283) 1 1 2.35365 1.9            
                               0.123113 1)                                     
                         #250=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)    
                                       (LOGPEN 0 0 8437824) 1                  
                                       (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Apa}I (2434)" "@N (@S)" 5
            -11.2043                                -9.26456 0 -10 2.91758
            0.711604 #249#)                          #251=(CScratch            
                                                           (CWidget 2 (1 #1# 2)
            1 2 0 0 #189# 29277648                                 100) (LOGPEN
            0 0 10079283) 1 1 1.28235 1.9                               
            0.123113 1)                                                  
            #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Apa}I (3099)" "@N (@S)" 5 -14.1037        
                                   4.63228 0 -10 2.91758 0.711604 #251#))      
                              (CObjectList))                                   
                        #253=(CGroupWidget                                     
                              (CWidget 2 (10 #7# 0) 1 2 0 0 Nil 4129200 100)   
                                 (CObjectList                                  
                                  #254=(CScratch                               
                                        (CWidget 1 (1 #7# 1) 1 2 0 0 #189# 0
            100)                                (LOGPEN 0 0 10079283) 1 1
            6.10725 1.9                                0.123113 1)             
                                                 #255=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #168# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 39
            14 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Eco}RV (104)" "@N (@S)" 5 8.17466                            
               11.5807 0 -10 3.14885 0.711604 #254#)                         
            #256=(CScratch                                                     
                  (CWidget 2 (1 #7# 2) 1 2 0 0 #189# 4129504                   
                         100) (LOGPEN 0 0 10079283) 1 1 0.89732 1.9            
                               0.123113 1)                                     
                         #257=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)    
                                       (LOGPEN 0 0 8437824) 1                  
                                       (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Eco}RV (3338)" "@N (@S)" 5
            -12.3794                                8.10649 0 -10 3.45128
            0.711604 #256#))                         (CObjectList))            
                                               #258=(CGroupWidget              
                                                     (CWidget 2 (10 #9# 0) 1 2
            0 0 Nil 4129200 100)                         (CObjectList          
                                                          #259=(CScratch       
                                                                (CWidget 1 (1
            #9# 1) 1 2 0 0 #189# 0 100)                                (LOGPEN
            0 0 10079283) 1 1 1.99118 1.9                               
            0.123113 1)                                                  
            #260=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Nco}I (2659)" "@N (@S)" 5 -14.3413        
                                   -2.31614 0 -10 2.91758 0.711604 #259#)      
                               #261=(CScratch                                  
                                     (CWidget 2 (1 #9# 2) 1 2 0 0 #189# 0 100) 
                                          (LOGPEN 0 0 10079283) 1 1 0.125657
            1.9                                0.123113 1)                     
                                         #262=(CLabel                          
                                               (CWidget 0 (0 0) 1 2 0 0 #168#
            1649361704 100)                                (LOGPEN 0 0 8437824)
            1                                              (LOGFONT 39 14 0 0
            400 255 0 0 0 3 2 1 34                                 "Arial")
            1.15807 0.611111 0                                        "{\\i
            Nco}I (3817)" "@N (@S)" 5 -4.00149                               
            13.8968 0 -10 2.91758 0.711604 #261#))                        
            (CObjectList))                                               
            #263=(CGroupWidget                                                 
                  (CWidget 2 (10 #12# 0) 1 2 0 0 Nil 4129200 100)              
                      (CObjectList                                             
                       #264=(CScratch                                          
                             (CWidget 1 (1 #12# 1) 1 2 0 0 #189# 0 100)        
                                   (LOGPEN 0 0 10079283) 1 1 5.72223 1.9       
                                    0.123113 1)                                
                              #265=(CLabel                                     
                                    (CWidget 0 (0 0) 1 2 0 0 #168# -1069 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Nru}I (343)" "@N (@S)" 5
            9.60891                                10.4226 0 -10 2.50841
            0.711604 #264#)                          #266=(CScratch            
                                                           (CWidget 2 (1 #12#
            2) 1 2 0 0 #189# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 1.86713 1.9                                0.123113
            1)                                                   #267=(CLabel  
                                                                       (CWidget
            0 (0 0) 1 2 0 0 #168# 29007420 100)                               
            (LOGPEN 0 0 8437824) 1                                             
            (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                          
                  "Arial") 1.15807 0.611111 0                                  
                 "{\\i Nru}I (2736)" "@N (@S)" 5 -14.4121                      
                     1.15807 0 -10 2.81084 0.711604 #266#))                    
                (CObjectList))) (CObjectList))                        
            #268=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -800 100)        
                     (CObjectList) (CObjectList))                              
               #269=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 0 100)         
                     (CObjectList) (CObjectList))) (CObjectList)))        
            (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList) 10   
            (CStringList) 27693464 0 (CObList))).
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
     misc_feature    48
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             121..1086
                     /label="LacI"
     misc_feature    427
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     582..602
                     /%GC="47.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.4"
                     /annotation_group="605429400600680004ef5dfc-e589-467c-8df0
                     -cdce6a99a2ef"
                     /created_by="primer3"
                     /Sequence="TTTCTCCCATGAAGACGGTAC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P62"
     misc_feature    959
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             1087..1449
                     /label="ATOG4"
     primer_bind     1192..1211
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.0"
                     /annotation_group="60542940388386001047fc70-cd13-4f05-8f89
                     -a417adb1ced3"
                     /created_by="primer3"
                     /Sequence="ACTCAGACCAACTCTCAGGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P63"
     attL2           complement(1451..1550)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
     primer          complement(1588..1608)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     misc_feature    1661..1662
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Replacement"
     promoter        1669..1719
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1720..2529
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1745..1764
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="6054293829271400d0ec181f-3782-4ba1-9f13
                     -d13713ec684c"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P44"
     primer_bind     2379..2398
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6054293705801300dce95987-4073-4ae6-bb86
                     -36aa7e9189f2"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P42"
     rep_origin      2676..3264
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     3164..3183
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="6054293887667800b9b58643-6acb-4e38-ba71
                     -b8ea11b62c32"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P45"
     primer_bind     3772..3791
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60542939474879009a48ce8d-9a86-4402-b176
                     -dcf40b0b15c8"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-LHOATG4-L2"
                     /label="P46"
     primer          3863..3883
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taatgaaacc ggtaacgtta
      121 tacgacgtcg ctgaatacgc cggcgtttct catcaaaccg tttctagagt ggttaaccag
      181 gcttcacatg ttagcgctaa aacccgggaa aaagttgaag ctgccatggc tgagctcaac
      241 tacatcccga accgtgttgc gcagcagctg gctggtaaac aaagcttgct gatcggtgtc
      301 gcgacctcga gcttggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
      361 gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
      421 tgtaaatcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
      481 ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
      541 tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
      601 acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
      661 ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
      721 cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
      781 caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
      841 gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
      901 gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta
      961 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     1021 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     1081 aaaaccacta gtggatcgga attcgctaac ttcaaccagt ccggaaacat cgctgattct
     1141 tccttgagct tcactttcac taactcttct aacggaccta accttatcac cactcagacc
     1201 aactctcagg ctcttagcca gccaatcgct agctctaacg tgcacgacaa cttcatgaac
     1261 aacgagatca ctgctagcaa gatcgatgat ggtaacaatt ctaagcctct tagcccagga
     1321 tggactgatc agactgctta caacgcattc ggtatcacta ccggtatgtt caacaccact
     1381 accatggacg atgtgtacaa ctacctcttc gacgatgagg atactccacc taaccctaag
     1441 aaggagtgat acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1501 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1561 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1621 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1681 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1741 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1801 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1861 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1921 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1981 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2041 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2101 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2161 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2221 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2281 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2341 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2401 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2461 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2521 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2581 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2821 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3301 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3361 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3421 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3481 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3541 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3601 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3661 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3721 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3781 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3841 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
