LOCUS       pEN-R2-9-L3             2914 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...151-dt4t59o
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459272300|                                                 
            VNTDBDATE|459272300|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-R2-9-L3,0|                                             
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            0 0 Nil -260 100)                         (CObjectList             
                                                       #234=(CScratch          
                                                             (CWidget 1 (1 #14#
            1) 1 2 0 0 #182# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 6.0767 1.9 0.123113                                1)
                                                                      
            #235=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #161# 4129360 100)                  
                         (LOGPEN 0 0 8437824) 1                                
                         (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1 34             
                               "Arial") 1.15807 0.611111 0                     
                              "{\\i Sac}I (96)" "@N (@S)" 5 4.70391 13.8968    
                                       0 -10 2.32108 0.68832 #234#)            
                                 #236=(CScratch                                
                                       (CWidget 2 (1 #14# 2) 1 2 0 0 #182# 0
            100)                                (LOGPEN 0 0 10079283) 1 1
            1.1686 1.9 0.123113                                1)              
                                                         #237=(CLabel          
                                                               (CWidget 0 (0 0)
            1 2 0 0 #161# 4129168 100)                                (LOGPEN 0
            0 8437824) 1                                              (LOGFONT
            43 16 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Sac}I (2469)" "@N (@S)" 5 -13.6785                           
                5.79035 0 -10 2.96138 0.68832 #236#))                        
            (CObjectList))) (CObjectList))                        
            #238=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1098 100)       
                      (CObjectList) (CObjectList))                             
                #239=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1026 100)    
                         (CObjectList) (CObjectList))) (CObjectList)))        
            (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
            4129512    (CStringList) 50856168 4129472 (CObList))).
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     misc_feature    1^2
                     /Original_Bases="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGT
                     CTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT
                     CGTTCAACTTTCTTGTACAAAGT"
                     /label="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTT
                     ATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACT
                     TTCTTGTACAAAGT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    126..127
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     terminator      128..399
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tG7 (<a
                     href=""urn:local:.:6a-dgycryw"">G7T</a>)"
                     /label="tG7"
     misc_feature    400
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     attL3           complement(401..496)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     primer_bind     521..540
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.2"
                     /Tm="59.3"
                     /annotation_group="60535079017344000edee307-d836-4196-8bd8
                     -986e89ba53f3"
                     /created_by="primer3"
                     /Sequence="GGCCGCGATATCCCCTATAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-9-L3"
                     /label="P40"
     primer          complement(554..574)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        635..685
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    686..1495
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1345..1364
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60535079608451002edeaf16-7193-4d67-89b1
                     -cc8c03b0e032"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-9-L3"
                     /label="P42"
     rep_origin      1642..2230
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    1789
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2034..2035
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2130..2149
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="605350813482390022418d86-b922-465d-b65c
                     -ae846c088e2f"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-9-L3"
                     /label="P45"
     primer_bind     2738..2757
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="605350819586960054637fd3-c03f-42f0-9a7b
                     -eaff728eedd6"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-9-L3"
                     /label="P46"
     primer          2829..2849
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
     misc_feature    2913
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggaccc cgatgagcta agctagctat atcatcaatt tatgtattac acataatatc
      181 gcactcagtc tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt
      241 ctgaatttaa acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg
      301 ttattttatc aataaatatt taaactatat ttctttcaag atgggaatta acatctacaa
      361 attgcctttt cttatcgacc atgtactcga cggccgagta caactttatt atacaaagtt
      421 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
      481 aataaaatca ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag
      541 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
      601 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      661 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
      721 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      781 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
      841 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      901 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      961 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     1021 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1081 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     1141 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1201 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     1261 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1321 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1381 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1441 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     1501 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     1561 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     1621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     2161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     2221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     2281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     2341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     2401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2521 tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg
     2581 gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg
     2641 ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg
     2701 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt
     2761 atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
     2821 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat
     2881 tctacaggtc actaatacca tctaagtagt tggt
//
