LOCUS       pEN-R2-6-L3             2910 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...y0-dt4nhn9
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459271037|                                                 
            VNTDBDATE|459271170|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-R2-6-L3,0|                                             
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            0.704863 #232#))                         (CObjectList))            
                                               #234=(CGroupWidget              
                                                     (CWidget 2 (10 #14# 0) 1 2
            0 0 Nil -261 100)                         (CObjectList             
                                                       #235=(CScratch          
                                                             (CWidget 1 (1 #14#
            1) 1 2 0 0 #182# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 6.07644 1.9                                0.123113
            1)                                                   #236=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #161# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Sac}I (96)" "@N (@S)" 5 4.7072 13.8968                 
                          0 -10 2.29081 0.704863 #235#)                        
                    #237=(CScratch                                             
                          (CWidget 2 (1 #14# 2) 1 2 0 0 #182# 0 100)           
                                (LOGPEN 0 0 10079283) 1 1 1.16185 1.9          
                                 0.123113 1)                                   
                           #238=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #161# 1944788432 100)
                                           (LOGPEN 0 0 8437824) 1              
                                           (LOGFONT 39 14 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Sac}I (2469)" "@N (@S)" 5
            -13.644                                5.79035 0 -10 2.88994
            0.704863 #237#))                         (CObjectList)))
            (CObjectList))                         #239=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -1100 100)                  (CObjectList)
            (CObjectList))                                  #240=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -1027 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 42123616   
            (CStringList) 42123100 42124392 (CObList))).
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     misc_feature    1^2
                     /Original_Bases="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGT
                     CTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT
                     CGTTCAACTTTCTTGTACAAAGT"
                     /label="CATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTT
                     ATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAACT
                     TTCTTGTACAAAGT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    126..127
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     terminator      128..395
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     misc_feature    396
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     attL3           complement(397..492)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     primer_bind     517..536
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.2"
                     /Tm="59.3"
                     /annotation_group="60530939983467004922f7d0-cf84-4889-a03b
                     -f12a7be475fc"
                     /created_by="primer3"
                     /Sequence="GGCCGCGATATCCCCTATAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-6-L3"
                     /label="P40"
     primer          complement(550..570)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        631..681
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    682..1491
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1341..1360
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6053094056750300bf36c071-ebbb-4465-8057
                     -e9fb44ffbcfe"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-6-L3"
                     /label="P42"
     rep_origin      1638..2226
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    1785
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2030..2031
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2126..2145
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="60530942332018008bdd4e0e-1e8b-44bf-9c98
                     -fb4ccc4f1672"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-6-L3"
                     /label="P45"
     primer_bind     2734..2753
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6053094292412500920fb3d6-4cce-42db-9777
                     -8b18905af11c"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-6-L3"
                     /label="P46"
     primer          2825..2845
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
     misc_feature    2909
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggacta gagtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga
      181 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
      241 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
      301 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
      361 tatcgcgcgc ggtgtcatct atgttactag atcgaacaac tttattatac aaagttggca
      421 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
      481 aaatcattat ttggagctcc atggtagcgt taacgcggcc gcgatatccc ctatagtgag
      541 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
      601 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      661 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
      721 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      781 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
      841 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
      901 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      961 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1021 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1081 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1141 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1201 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1261 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1321 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1381 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1441 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     1501 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     1561 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     1621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2341 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2401 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2461 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     2521 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     2581 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     2641 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     2701 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     2761 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     2821 cgttgtaaaa cgacggccag tcttaagctc gggccctgca gctctagagc tcgaattcta
     2881 caggtcacta ataccatcta agtagttggt
//
