LOCUS       pEN-L1-L+-L2            4196 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...so-dt4idv1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459369339|                                                 
            VNTDBDATE|459369339|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L1-L+-L2,0|                                            
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                        (LOGBRUSH 0 16777215 0) 1                              
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                                        #156=(CWidgetStyle "5'UTR Label" 1
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               0.246225 1.15807 0.164644 2.53336                               
               (15 (CShapeMapEntry 8 "Shape 22" 1 "Label 3") 4                 
                (CShapeMapEntry 0 "Shape 33" 1 "Label 0") 29                   
                (CShapeMapEntry 0 "Shape 16" 1 "Label 1") 22                   
                (CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21                   
                (CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43                   
                (CShapeMapEntry 8 "Shape 20" 1 "Label 0") 52                   
                (CShapeMapEntry 9 "Shape 24" 1 "5'UTR Label") 50               
                (CShapeMapEntry 0 "Shape 32" 1 "Signal Label") 70              
                (CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68               
                (CShapeMapEntry 0 "RSite" 1 "Label 5") 67                      
                (CShapeMapEntry 0 "ORF" 0 "ORF Label") 94                      
                (CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88                    
                (CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87                   
                (CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86                   
                (CShapeMapEntry 8 "Shape 19" 1 "Label 0") 85                   
                (CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449 39
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                       (mapper: 17.1567 -14.1544 67.6449 67.6449 0.01 10 -1
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                                           #160=(CGroupWidget (CWidget 1 (0 0)
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            #163=(CLabel                                                       
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                        (CObjectList))                                         
                  #164=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1842 100)  
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                            #165=(CGroupWidget                                 
                                  (CWidget 4 (7 4 0) 1 2 0 0 Nil -197 100)     
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                           (CObjectList))                                      
                     #168=(CGroupWidget                                        
                           (CWidget 22 (7 22 0) 1 2 0 0 Nil -275 100)          
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                                          (LOGBRUSH 0 13421823 0) 1 4.67461
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                                               #170=(CLabel                    
                                                     (CWidget 0 (0 0) 1 2 0 0
            #126# 21818956 100)                                (LOGPEN 0 0
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            "Arial") 1.15807 0.666667 128 "Kan(R)" "@N"                        
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                       (CWidget 86 (7 86 0) 1 2 0 0 Nil -902 100)              
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            1                                0.123113)                         
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                                             (CWidget 0 (0 0) 1 2 0 0 #126#
            67779700 100)                                (LOGPEN 0 0 3342591) 1
                                                         (LOGFONT 52 19 0 0 700
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            0.666667 128 "attL1" "@N" 1                                -8.98934
            -10.4226 0 -10 1.40439 0.739154                               
            #172#)                                                       
            #174=(CWideLine                                                    
                  (CWidget 0 (3 #25# 0) 1 2 0 0 #149# 21498584                 
                           100) (LOGPEN 0 0 10066329) 1                        
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            128 "attL2" "@N" 1                                3.82292 12.7388 0
            -10 1.40439 0.739154 #174#))                        
            (CObjectList))) (CObjectList))                        
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                      #177=(CGroupWidget                                       
                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -120 100)        
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                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #112# 452 100) 
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                                                       (CWidget 0 (0 0) 1 2 0 0
            #141# 14352920 100)                                (LOGPEN 0 0
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            "Arial") 1.15807 0.611111 0                                       
            "{\\i Apa}I (2434)" "@N (@S)" 5 -8.5917                            
               -11.5807 0 -10 3.11923 0.694805 #178#))                        
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            #180=(CGroupWidget                                                 
                  (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -512 100)                  
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                   #181=(CScratch                                              
                         (CWidget 1 (1 #7# 1) 1 2 0 0 #112# 0 100)             
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                                     (CWidget 0 (0 0) 1 2 0 0 #141# 21851060
            100)                                (LOGPEN 0 0 8437824) 1         
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            0.611111 0                                        "{\\i Eco}RV
            (104)" "@N (@S)" 5 8.2731                                11.5807 0
            -10 3.35576 0.694805 #181#))                         (CObjectList))
                                                           #183=(CGroupWidget  
                                                                 (CWidget 1 (10
            #12# 0) 1 2 0 0 Nil -669 100)                         (CObjectList 
                                                                  
            #184=(CScratch                                                     
                  (CWidget 1 (1 #12# 1) 1 2 0 0 #112# 0 100)                   
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                               (CWidget 0 (0 0) 1 2 0 0 #141# 29819252 100)    
                                       (LOGPEN 0 0 8437824) 1                  
                                       (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1
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                                              "{\\i Nru}I (343)" "@N (@S)" 5
            9.70734                                10.4226 0 -10 2.72009
            0.694805 #184#))                         (CObjectList))            
                                               #186=(CGroupWidget              
                                                     (CWidget 1 (10 #19# 0) 1 2
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                               "{\\i Sph}I (3199)" "@N (@S)" 5 -14.5674        
                                   1.15807 0 -10 3.11923 0.694805 #187#))      
                              (CObjectList))                                   
                        #189=(CGroupWidget                                     
                              (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -1687 100)     
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                                                       (CWidget 0 (0 0) 1 2 0 0
            #140# 6751096 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
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            "Arial") 1.15807 0.611111 0                                       
            "{\\i Ava}I (2427)" "@N (@S)" 5 -8.53879                           
                -12.7388 0 -10 3.07488 0.694805 #190#)                         
            #192=(CScratch                                                     
                  (CWidget 2 (1 #3# 2) 1 2 0 0 #112# 1816403032                
                            100) (LOGPEN 0 10 0) 8 1 0.903219 1.9              
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                       #193=(CLabel (CWidget 0 (0 0) 1 2 0 0 #140# 0 100)      
                                     (LOGPEN 0 0 8437824) 1                    
                                     (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34 
                                           "Arial") 1.15807 0.611111 0         
                                          "{\\i Ava}I (3592)" "@N (@S)" 5
            -12.1895                                8.10649 0 -10 3.07488
            0.694805 #192#))                         (CObjectList)))
            (CObjectList))                         #194=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -947 100)                  (CObjectList)
            (CObjectList))                                  #195=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -512 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 27903968   
            (CStringList) 27393512 27904004 (CObList))).
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
     misc_feature    101..102
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             103..1752
                     /label="Luc+"
     primer_bind     575..595
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /Sequence="ACCAGGGATTTCAGTCGATGT"
                     /Tm="59.1"
                     /%GC="47.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P54"
     primer_bind     1376..1395
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /Sequence="ACTGGGACGAAGACGAACAC"
                     /Tm="60.0"
                     /%GC="55.0"
                     /Hairpin_Tm="42.7"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P55"
     attL2           complement(1754..1852)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="99.00%"
                     /Primary_Match="99.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
     misc_feature    1758^1759
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     primer          complement(1890..1910)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     misc_feature    1963..1964
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Replacement"
     promoter        1971..2021
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2022..2831
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     2047..2066
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="605273661785460019253fd6-4702-401f-adc7
                     -e8f0c8776e1d"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /label="P44"
     primer_bind     2681..2700
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6052736501771300abca8849-cbaa-4989-9565
                     -6199c469cdab"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /label="P42"
     rep_origin      2978..3566
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     3466..3485
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="605273667663810079057ce4-356a-49e4-80d9
                     -3dee03924022"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /label="P45"
     primer_bind     4074..4093
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="605273673619320033168b65-9fb5-4cf5-8949
                     -7ea9ba6eff7e"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L1-L+-L2"
                     /label="P46"
     primer          4165..4185
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taatggaaga cgccaaaaac
      121 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      181 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      241 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      301 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      361 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      421 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      481 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
      541 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
      601 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
      661 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
      721 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
      781 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
      841 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
      901 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
      961 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     1021 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     1081 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     1141 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     1201 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     1261 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     1321 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     1381 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     1441 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     1501 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     1561 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     1621 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     1681 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     1741 aagatcgccg tgtacccact ttcttgtaca aagttggcat tataagaaag cattgcttat
     1801 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1861 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1921 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1981 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2041 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2101 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2161 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2221 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2281 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2341 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2401 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2461 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2521 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     2581 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2641 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2701 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2761 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     2821 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2881 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2941 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3001 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3061 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3121 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3181 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3241 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3301 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3361 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3421 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3481 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3541 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3601 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3661 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3721 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3781 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3841 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3901 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3961 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4021 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4081 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     4141 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccc
//
