LOCUS       pEN-L4-UBIL-R1          4639 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...rg-dozejd1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459276748|                                                 
            VNTDBDATE|459276748|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L4-UBIL-R1,0|                                          
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                               "{\\i Bgl}II (1034)" "@N (@S)" 5 14.3422        
                                   2.31614 0 -10 2.91758 0.711604 #235#))      
                              (CObjectList))                                   
                        #237=(CGroupWidget                                     
                              (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -306 100)      
                              (CObjectList                                     
                               #238=(CScratch                                  
                                     (CWidget 1 (1 #1# 1) 1 2 0 0 #189# 2949135
                                            100) (LOGPEN 0 0 10079283) 1 1
            4.72013 1.9                                0.123113 1)             
                                                 #239=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #168# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 39
            14 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Apa}I (1147)" "@N (@S)" 5 14.5041                            
               -1.15807 0 -10 2.91758 0.711604 #238#)                         
            #240=(CScratch                                                     
                  (CWidget 2 (1 #1# 2) 1 2 0 0 #189# 1441811                   
                         100) (LOGPEN 0 0 10079283) 1 1 0.0338798 1.9          
                                 0.123113 1)                                   
                           #241=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Apa}I (4605)" "@N (@S)" 5
            -8.00674                                11.5807 0 -10 2.91758
            0.711604 #240#))                         (CObjectList))            
                                               #242=(CGroupWidget              
                                                     (CWidget 2 (10 #3# 0) 1 2
            0 0 Nil -631 100)                         (CObjectList             
                                                       #243=(CScratch          
                                                             (CWidget 1 (1 #3#
            1) 1 2 0 0 #189# 1986293549                                 100)
            (LOGPEN 0 0 10079283) 1 1 5.20665 1.9                              
             0.123113 1)                                                  
            #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 2 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Ava}I (788)" "@N (@S)" 5 13.6261          
                                 4.63228 0 -10 2.61515 0.711604 #243#)         
                            #245=(CScratch                                     
                                  (CWidget 2 (1 #3# 2) 1 2 0 0 #189# 25373384  
                                          100) (LOGPEN 0 0 10079283) 1 1
            0.0433662 1.9                                0.123113 1)           
                                                   #246=(CLabel                
                                                         (CWidget 0 (0 0) 1 2 0
            0 #168# 327728 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 39
            14 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Ava}I (4598)" "@N (@S)" 5 -9.80578                           
                10.4226 0 -10 2.91758 0.711604 #245#))                        
            (CObjectList))                                               
            #247=(CGroupWidget                                                 
                  (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -240 100)                  
                  (CObjectList                                                 
                   #248=(CScratch                                              
                         (CWidget 1 (1 #6# 1) 1 2 0 0 #189# 28750392           
                                 100) (LOGPEN 0 0 10079283) 1 1 4.26344 1.9    
                                       0.123113 1)                             
                                 #249=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 39 14 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Eco}RI
            (1484)" "@N (@S)" 5 13.6369                                -5.79035
            0 -10 3.27338 0.711604 #248#)                         
            #250=(CScratch                                                     
                  (CWidget 2 (1 #6# 2) 1 2 0 0 #189# 875390578                 
                           100) (LOGPEN 0 0 10079283) 1 1 3.23755 1.9          
                                 0.123113 1)                                   
                           #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Eco}RI (2241)" "@N (@S)" 5
            8.21519                                -11.5807 0 -10 3.27338
            0.711604 #250#))                         (CObjectList))            
                                               #252=(CGroupWidget              
                                                     (CWidget 2 (10 #9# 0) 1 2
            0 0 Nil -761 100)                         (CObjectList             
                                                       #253=(CScratch          
                                                             (CWidget 1 (1 #9#
            1) 1 2 0 0 #189# 25313348                                 100)
            (LOGPEN 0 0 10079283) 1 1 4.73775 1.9                              
             0.123113 1)                                                  
            #254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Nco}I (1134)" "@N (@S)" 5 14.5123 0 0     
                                      -10 2.91758 0.711604 #253#)              
                                #255=(CScratch                                 
                                      (CWidget 2 (1 #9# 2) 1 2 0 0 #189#
            28092400                                 100) (LOGPEN 0 0 10079283)
            1 1 0.0149071 1.9                                0.123113 1)       
                                                       #256=(CLabel (CWidget 0
            (0 0) 1 2 0 0 #168# 0 100)                                (LOGPEN 0
            0 8437824) 1                                              (LOGFONT
            39 14 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Nco}I (4619)" "@N (@S)" 5 -4.65673                           
                12.7388 0 -10 2.91758 0.711604 #255#))                        
            (CObjectList))                                               
            #257=(CGroupWidget                                                 
                  (CWidget 2 (10 #14# 0) 1 2 0 0 Nil -1607 100)                
                    (CObjectList                                               
                     #258=(CScratch                                            
                           (CWidget 1 (1 #14# 1) 1 2 0 0 #189# 25314348        
                                    100) (LOGPEN 0 0 10079283) 1 1 3.224 1.9   
                                        0.123113 1)                            
                                  #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 39 14 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sac}I
            (2251)" "@N (@S)" 5 4.63936                                -12.7388
            0 -10 2.91758 0.711604 #258#)                         
            #260=(CScratch                                                     
                  (CWidget 2 (1 #14# 2) 1 2 0 0 #189# 1 100)                   
                        (LOGPEN 0 0 10079283) 1 1 0.00271038 1.9               
                            0.123113 1)                                        
                      #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #168# 0 100)       
                                    (LOGPEN 0 0 8437824) 1                     
                                    (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34  
                                          "Arial") 1.15807 0.611111 0          
                                         "{\\i Sac}I (4628)" "@N (@S)" 5
            -2.39566                                13.8968 0 -10 2.91758
            0.711604 #260#))                         (CObjectList)))
            (CObjectList))                         #262=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -175 100)                  (CObjectList)
            (CObjectList))                                  #263=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -827 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 39536440   
            (CStringList) 268747808 39536572 (CObList))).
FEATURES             Location/Qualifiers
     attL4           1..97
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
     misc_feature    99..105
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        105..2092
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /label="ZmUBIL"
     primer_bind     345..367
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /Sequence="TTGACAACAGGACTCTACAGTTT"
                     /Tm="57.6"
                     /%GC="39.1"
                     /Hairpin_Tm="30.6"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P51"
     misc_feature    911
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     intron          1083..2093
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.80%"
                     /Primary_Match="99.80%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     misc_feature    1112
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     1135..1154
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /Sequence="GTTCCGGTCCATGGTTAGGG"
                     /Tm="60.1"
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="12.6"
                     /modified_by="mamee"
                     /label="P52"
     primer_bind     1725..1746
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /Sequence="ACTGTTTCAAACTACCTGGTGT"
                     /Tm="57.8"
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="10.2"
                     /modified_by="mamee"
                     /label="P53"
     misc_feature    2093
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     attR1           2094..2250
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="99.36%"
                     /Primary_Match="99.36%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:y-f0o11e8"">attR1</a>"
     misc_feature    2220
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer          complement(2313..2329)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     promoter        2391..2441
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2442..3251
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     2467..2486
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="60526097104382000e02f64f-718f-4a5d-835e
                     -35a396b1814c"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /label="P44"
     primer_bind     3101..3120
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60526095879688007865f609-cf58-4c9e-b140
                     -639ca0a64b2d"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /label="P42"
     rep_origin      3398..3986
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    3545
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    3790..3791
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     3886..3905
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="60526097711379006d85aa1d-5132-4255-acc6
                     -eb11f1285136"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /label="P45"
     primer_bind     4494..4513
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6052609832278000782c2b58-c64c-458f-9ade
                     -ed59d52617b8"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-UBIL-R1"
                     /label="P46"
     primer          4565..4581
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          4585..4600
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     misc_feature    4618
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttggac tgcagtgcag cgtgacccgg
      121 tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa attaccacat
      181 attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac
      241 tttactctac gaataatata atctatagta ctacaataat atcagtgttt tagagaatca
      301 tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct
      361 acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta
      421 tataatactt catccatttt attagtacat ccatttaggg tttagggtta atggttttta
      481 tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact
      541 aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt
      601 gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt
      661 gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg gacaccaacc
      721 agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct
      781 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc
      841 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct
      901 ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct
      961 cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc
     1021 ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac ctccgcttca
     1081 aggtacgccg ctcgtcctcc cccccccccc cctctctacc ttctctagat cggcgttccg
     1141 gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg
     1201 tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct
     1261 gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc
     1321 agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt
     1381 cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt
     1441 gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg
     1501 tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc
     1561 atagttacga attgaagatg atggatggaa atatcgatct aggataggta tacatgttga
     1621 tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg
     1681 gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc
     1741 tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt
     1801 ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga
     1861 tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc
     1921 tattataata aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc
     1981 atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt
     2041 ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg caacaagttt
     2101 gtacaaaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat
     2161 tttgcataaa aaacagacta cataatactg taaaacacaa catatgcagt cactatgaac
     2221 caactactta gatggtatta gtgacctgta gaattcgagc tctagagctg cagggcggcc
     2281 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     2341 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     2401 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     2461 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2521 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     2581 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2641 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2701 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     2761 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2821 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2881 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2941 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     3001 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     3061 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     3121 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     3181 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3241 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     3301 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     3361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4081 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4141 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4201 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4261 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     4321 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     4381 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     4441 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     4501 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     4561 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggcccgagt
     4621 taacgctacc atggagctc
//
