LOCUS       pEN-L4-3-R1             3016 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...q6-dt3haey
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459284798|                                                 
            VNTDBDATE|459284798|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-L4-3-R1,0|                                             
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                          (CWidget 1 (1 #14# 1) 1 2 0 0 #179# 0 100)           
                                (LOGPEN 0 0 10079283) 1 1 5.94161 1.9          
                                 0.123113 1)                                   
                           #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #158# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sac}I (162)" "@N (@S)" 5
            9.64944                                10.4226 0 -10 2.61515
            0.711604 #233#)                          #235=(CScratch            
                                                           (CWidget 2 (1 #14#
            2) 1 2 0 0 #179# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 1.04622 1.9                                0.123113
            1)                                                   #236=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #158# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Sac}I (2539)" "@N (@S)" 5 -13.1418                     
                      8.10649 0 -10 2.91758 0.711604 #235#))                   
                 (CObjectList))                                               
            #237=(CGroupWidget                                                 
                  (CWidget 2 (10 #19# 0) 1 2 0 0 Nil -437 100)                 
                   (CObjectList                                                
                    #238=(CScratch                                             
                          (CWidget 1 (1 #19# 1) 1 2 0 0 #179# 0 100)           
                                (LOGPEN 0 0 10079283) 1 1 0.825853 1.9         
                                  0.123113 1)                                  
                            #239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #158# 0 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 39 14 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Sph}I (2646)" "@N (@S)" 5
            -11.6354                                10.4226 0 -10 2.91758
            0.711604 #238#)                          #240=(CScratch            
                                                           (CWidget 2 (1 #19#
            2) 1 2 0 0 #179# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 0.817615 1.9                                0.123113
            1)                                                   #241=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #158# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 39 14 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Sph}I (2650)" "@N (@S)" 5 -11.6512                     
                      11.5807 0 -10 2.91758 0.711604 #240#))                   
                 (CObjectList))) (CObjectList))                        
            #242=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -958 100)        
                     (CObjectList) (CObjectList))                              
               #243=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -998 100)      
                       (CObjectList) (CObjectList))) (CObjectList)))        
            (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
            40232936    (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     attL4           1..97
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
     misc_feature    99^100
                     /Original_Bases="CTGCATGCATGCTGCAGGTCGACTCTCTAG"
                     /label="CTGCATGCATGCTGCAGGTCGACTCTCTAG"
                     /note="Geneious type: Editing History Deletion"
     promoter        100..469
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pROLD
                     (<a href=""urn:local:.:uf-f190xjm"">pROLD - Gateway</a>)"
                     /label="pROLD"
     attR1           471..627
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="99.36%"
                     /Primary_Match="99.36%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:y-f0o11e8"">attR1</a>"
     misc_feature    597
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     654..673
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.2"
                     /Tm="59.3"
                     /annotation_group="605252364708030069497915-9cd7-409a-b34b
                     -c7b12758d06a"
                     /created_by="primer3"
                     /Sequence="GGCCGCGATATCCCCTATAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-3-R1"
                     /label="P40"
     primer          complement(687..707)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        768..818
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    819..1628
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1478..1497
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6052523707925000ac02438b-3dc7-411e-a66d
                     -3344be57d6e8"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-3-R1"
                     /label="P42"
     rep_origin      1775..2363
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    1922
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2167..2168
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2263..2282
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="6052523880857300f39ba94c-c2c1-4dd3-a0c3
                     -d333be1cd0ac"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-3-R1"
                     /label="P45"
     primer_bind     2871..2890
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60525239387437004d0e0c40-b911-424d-a1ea
                     -44ef5586058c"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-L4-3-R1"
                     /label="P46"
     primer          2962..2982
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
     misc_feature    2995
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttggag aatttgttcg tgaactatta
      121 gttgcgggcc ttggcatccg actacctctg cggcaatatt atattccctg ggcccaccgt
      181 gaacccaatt tcgcctattt attcattacc cccattaaca ttgaagtagt catgatgggc
      241 ctgcagcacg ttggtgaggc tggcacaact catccatata ctttctgacc ggatcggcac
      301 attattgtag aaaacgcgga cccacagcgc actttccaaa gcggtgccgc gtcagaatgc
      361 gctggcagaa aaaaattaat ccaaaagtac cctccaagca gcccatataa acgcgtttac
      421 aaatccgcta acctcaacaa tttgagcaga gaaaattcgc tagaggatca caagtttgta
      481 caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
      541 gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa
      601 ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag ggcggccgcg
      661 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
      721 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
      781 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
      841 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
      901 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
      961 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     1021 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     1081 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     1141 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     1201 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     1261 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     1321 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     1381 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     1441 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
     1501 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
     1561 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
     1621 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
     1681 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1921 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2461 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     2521 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2581 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2641 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     2701 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     2761 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     2821 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     2881 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     2941 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccgagttaa
     3001 cgctaccatg gagctc
//
