LOCUS       pEN-R2-R-L3             3317 bp    DNA     circular     06-SEP-2022
DEFINITION  .
ACCESSION   urn.local...qr-dozds7b
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|459291457|                                                 
            VNTDBDATE|459291457|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-R2-R-L3,0|                                             
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                                    11.5807 0 -10 2.97739 0.704328 #234#))     
                               (CObjectList))                                  
                         #236=(CGroupWidget                                    
                               (CWidget 2 (10 #3# 0) 1 2 0 0 Nil -839 100)     
                               (CObjectList                                    
                                #237=(CScratch                                 
                                      (CWidget 1 (1 #3# 1) 1 2 0 0 #186# 0 100)
                                           (LOGPEN 0 0 10079283) 1 1 1.64915
            1.9                                0.123113 1)                     
                                         #238=(CLabel (CWidget 0 (0 0) 1 2 0 0
            #165# 0 100)                                (LOGPEN 0 0 8437824) 1 
                                                        (LOGFONT 43 16 0 0 400
            255 0 0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Ava}I
            (2446)" "@N (@S)" 5 -14.5798                               
            -2.31614 0 -10 2.92936 0.704328 #237#)                         
            #239=(CScratch                                                     
                  (CWidget 2 (1 #3# 2) 1 2 0 0 #186# 0 100)                    
                       (LOGPEN 0 0 10079283) 1 1 0.796205 1.9                  
                         0.123113 1)                                           
                   #240=(CLabel (CWidget 0 (0 0) 1 2 0 0 #165# 0 100)          
                                 (LOGPEN 0 0 8437824) 1                        
                                 (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1 34     
                                       "Arial") 1.15807 0.611111 0             
                                      "{\\i Ava}I (2897)" "@N (@S)" 5 -11.3663 
                                          9.26456 0 -10 2.92936 0.704328
            #239#))                         (CObjectList))                     
                                      #241=(CGroupWidget                       
                                            (CWidget 2 (10 #14# 0) 1 2 0 0 Nil
            -116 100)                         (CObjectList                     
                                               #242=(CScratch                  
                                                     (CWidget 1 (1 #14# 1) 1 2
            0 0 #186# 0 100)                                (LOGPEN 0 0
            10079283) 1 1 6.09352 1.9                                0.123113
            1)                                                   #243=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #165# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Sac}I (96)" "@N (@S)" 5 4.58288 13.8968                
                           0 -10 2.3691 0.704328 #242#)                        
                     #244=(CScratch                                            
                           (CWidget 2 (1 #14# 2) 1 2 0 0 #186# 0 100)          
                                 (LOGPEN 0 0 10079283) 1 1 1.60565 1.9         
                                  0.123113 1)                                  
                            #245=(CLabel (CWidget 0 (0 0) 1 2 0 0 #165# 0 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 43 16 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Sac}I (2469)" "@N (@S)" 5
            -14.6008                                1.15807 0 -10 2.97739
            0.704328 #244#))                         (CObjectList)))
            (CObjectList))                         #246=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -1101 100)                  (CObjectList)
            (CObjectList))                                  #247=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -1027 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 0    
            (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     CDS             128..802
                     /modified_by="mamee"
                     /label="RFP"
     primer_bind     357..376
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6052217027175600af1913ba-81f1-4ad0-b92c
                     -e11935f54e12"
                     /created_by="primer3"
                     /Sequence="CCGACATCCCCGACTACATG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-R-L3"
                     /label="P49"
     attL3           complement(804..899)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     primer          complement(960..976)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     promoter        1038..1088
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1089..1898
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1114..1133
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="25.9"
                     /Tm="59.8"
                     /annotation_group="605221685024710023be69b0-ddd8-442f-b044
                     -dcdbcd346a90"
                     /created_by="primer3"
                     /Sequence="CGAGGCCGCGATTAAATTCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-R-L3"
                     /label="P44"
     primer_bind     1748..1767
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="6052216734270600b88d8f51-c8e7-4211-aef9
                     -42cab7d323d7"
                     /created_by="primer3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-R-L3"
                     /label="P42"
     rep_origin      2045..2633
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    2192
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2437..2438
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2533..2552
                     /%GC="55.0"
                     /Hairpin_Tm="37.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="6052216908712200e73dde9e-b130-434a-a648
                     -7890142a0427"
                     /created_by="primer3"
                     /Sequence="GGGGAAACGCCTGGTATCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-R-L3"
                     /label="P45"
     primer_bind     3141..3160
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="60522169696914000890597b-447f-40db-ab77
                     -81b3745204c6"
                     /created_by="primer3"
                     /Sequence="CTTCCGACTGAGCCTTTCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-R-L3"
                     /label="P46"
     primer          3212..3228
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     primer          3232..3247
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     misc_feature    3316
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggaatg gacaacaccg aggacgtcat caaggagttc atgcagttca aggtgcgcat
      181 ggagggctcc gtgaacggcc actacttcga gatcgagggc gagggcgagg gcaagcccta
      241 cgagggcacc cagaccgcca agctgcaggt gaccaagggc ggccccctgc ccttcgcctg
      301 ggacatcctg tccccccagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga
      361 catccccgac tacatgaagc tgtccttccc cgagggcttc acctgggagc gctccatgaa
      421 cttcgaggac ggcggcgtgg tggaggtgca gcaggactcc tccctgcagg acggcacctt
      481 catctacaag gtgaagttca agggcgtgaa cttccccgcc gacggccccg taatgcagaa
      541 gaagactgcc ggctgggagc cctccaccga gaagctgtac ccccaggacg gcgtgctgaa
      601 gggcgagatc tcccacgccc tgaagctgaa ggacggcggc cactacacct gcgacttcaa
      661 gaccgtgtac aaggccaaga agcccgtgca gctgcccggc aaccactacg tggactccaa
      721 gctggacatc accaaccaca acgaggacta caccgtggtg gagcagtacg agcacgccga
      781 ggcccgccac tccggctccc agacaacttt attatacaaa gttggcatta taaaaaagca
      841 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
      901 gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg
      961 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1021 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1081 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1141 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1201 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1261 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1321 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1381 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1441 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     1501 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     1561 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     1621 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     1681 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     1741 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     1801 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     1861 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     1921 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     1981 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2161 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2821 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2881 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     2941 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     3001 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3061 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3121 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     3181 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     3241 cggccagtct taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata
     3301 ccatctaagt agttggt
//
