LOCUS       pEN-R2-TAPtag-L3        3205 bp    DNA     circular     08-JUL-2022
DEFINITION  .
ACCESSION   urn.local...qg-dozby8n
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is best viewed with a free Vector NTI Viewer:            
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|458702361|                                                 
            VNTDBDATE|459079603|                                               
            LSOWNER|                                                           
            VNTNAME|pEN-R2TAPtagL3,0|                                          
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Rebecca De Clerq|                                   
            VNTREPLTYPE|Plasmid                                                
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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            (LOGFONT 43 16 0 0 400 255 1 0 0 3 2 1 34                          
                  "Arial") 1.15807 0.611111 0                                  
                 "{\\i Eco}RV (1443)" "@N (@S)" 5 6.80502                      
                     -15.0549 0 -10 3.56966 0.704328 #238#))                   
                 (CObjectList))                                               
            #240=(CGroupWidget                                                 
                  (CWidget 1 (10 #9# 0) 1 2 0 0 Nil 0 100)                     
                  (CObjectList                                                 
                   #241=(CScratch                                              
                         (CWidget 1 (1 #9# 1) 1 2 0 0 #199# 3670472            
                                100) (LOGPEN 0 0 10079283) 1 1 3.50163 1.9     
                                      0.123113 1)                              
                                #242=(CLabel                                   
                                      (CWidget 0 (0 0) 1 2 0 0 #179# 24828656
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 43 16 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Nco}I
            (1417)" "@N (@S)" 5 6.96961                                -12.7388
            0 -10 3.04142 0.704328 #241#))                        
            (CObjectList))                                               
            #243=(CGroupWidget                                                 
                  (CWidget 1 (10 #10# 0) 1 2 0 0 Nil 4 100)                    
                  (CObjectList                                                 
                   #244=(CScratch                                              
                         (CWidget 1 (1 #10# 1) 1 2 0 0 #199# 3670472           
                                 100) (LOGPEN 0 0 10079283) 1 1 5.02833 1.9    
                                       0.123113 1)                             
                                 #245=(CLabel                                  
                                       (CWidget 0 (0 0) 1 2 0 0 #179# 24747672
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 43 16 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Nde}I
            (637)" "@N (@S)" 5 14.2834                                2.31614 0
            -10 2.73727 0.704328 #244#))                         (CObjectList))
                                                           #246=(CGroupWidget  
                                                                 (CWidget 1 (10
            #11# 0) 1 2 0 0 Nil 2 100)                           (CObjectList  
                                                                 
            #247=(CScratch                                                     
                  (CWidget 1 (1 #11# 1) 1 2 0 0 #199# 3670472                  
                          100) (LOGPEN 0 0 10079283) 1 1 3.46836 1.9           
                                0.123113 1)                                    
                          #248=(CLabel                                         
                                (CWidget 0 (0 0) 1 2 0 0 #179# 24735680 100)   
                                        (LOGPEN 0 0 8437824) 1                 
                                        (LOGFONT 43 16 0 0 400 255 1 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Not}I (1434)" "@N (@S)" 5
            6.56508                                -13.8968 0 -10 2.88134
            0.704328 #247#))                         (CObjectList))            
                                               #249=(CGroupWidget              
                                                     (CWidget 1 (10 #12# 0) 1 2
            0 0 Nil 2 100)                           (CObjectList              
                                                      #250=(CScratch           
                                                            (CWidget 1 (1 #12#
            1) 1 2 0 0 #199# 3670472                                 100)
            (LOGPEN 0 0 10079283) 1 1 2.98294 1.9                              
             0.123113 1)                                                  
            #251=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #179# 24734480 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 43 16 0 0 400 255 1 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Nru}I (1682)" "@N (@S)" 5 -4.70305        
                                   -12.7388 0 -10 2.92936 0.704328 #250#))     
                               (CObjectList))                                  
                         #252=(CGroupWidget                                    
                               (CWidget 1 (10 #13# 0) 1 2 0 0 Nil 6 100)       
                               (CObjectList                                    
                                #253=(CScratch                                 
                                      (CWidget 1 (1 #13# 1) 1 2 0 0 #199#
            3670472                                 100) (LOGPEN 0 0 10079283)
            1 1 5.1536 1.9                                0.123113 1)          
                                                    #254=(CLabel               
                                                          (CWidget 0 (0 0) 1 2
            0 0 #179# 1944545584 100)                                (LOGPEN 0
            0 8437824) 1                                              (LOGFONT
            43 16 0 0 400 255 1 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Pst}I (573)" "@N (@S)" 5 13.3384                             
              5.79035 0 -10 2.52918 0.704328 #253#))                        
            (CObjectList))                                               
            #255=(CGroupWidget                                                 
                  (CWidget 1 (10 #20# 0) 1 2 0 0 Nil 0 100)                    
                  (CObjectList                                                 
                   #256=(CScratch                                              
                         (CWidget 1 (1 #20# 1) 1 2 0 0 #199# 20471076          
                                  100) (LOGPEN 0 0 10079283) 1 1 5.14773 1.9   
                                        0.123113 1)                            
                                  #257=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 2
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 43 16 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xba}I
            (576)" "@N (@S)" 5 13.7245                                4.63228 0
            -10 2.73727 0.704328 #256#))                         (CObjectList))
                                                           #258=(CGroupWidget  
                                                                 (CWidget 2 (10
            #15# 0) 1 2 0 0 Nil 2 100)                           (CObjectList  
                                                                 
            #259=(CScratch                                                     
                  (CWidget 1 (1 #15# 1) 1 2 0 0 #199# 20458068                 
                           100) (LOGPEN 0 0 10079283) 1 1 4.4979 1.9           
                                0.123113 1)                                    
                          #260=(CLabel                                         
                                (CWidget 0 (0 0) 1 2 0 0 #178# 24262840 100)   
                                        (LOGPEN 0 0 8437824) 1                 
                                        (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sac}II (908)" "@N (@S)" 5
            14.3587                                -2.31614 0 -10 2.81731
            0.704328 #259#)                          #261=(CScratch            
                                                           (CWidget 2 (1 #15#
            2) 1 2 0 0 #199# 3670472                                 100)
            (LOGPEN 0 0 10079283) 1 1 3.73455 1.9                              
             0.123113 1)                                                  
            #262=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #178# 1944545584 100)               
                            (LOGPEN 0 0 8437824) 1                             
                            (LOGFONT 43 16 0 0 400 255 0 0 0 3 2 1 34          
                                  "Arial") 1.15807 0.611111 0                  
                                 "{\\i Sac}II (1298)" "@N (@S)" 5 10.0027      
                                     -10.4226 0 -10 3.12145 0.704328 #261#))   
                                 (CObjectList))                                
                           #263=(CGroupWidget                                  
                                 (CWidget 2 (10 #23# 0) 1 2 0 0 Nil 0 100)     
                                 (CObjectList                                  
                                  #264=(CScratch                               
                                        (CWidget 1 (1 #23# 1) 1 2 0 0 #199#
            3670448                                 100) (LOGPEN 0 0 10079283)
            1 1 4.36285 1.9                                0.123113 1)         
                                                     #265=(CLabel (CWidget 0 (0
            0) 1 2 0 0 #178# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 43
            16 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Bgl}II (977)" "@N (@S)" 5 13.7803                            
               -4.63228 0 -10 2.67324 0.704328 #264#)                         
            #266=(CScratch                                                     
                  (CWidget 2 (1 #23# 2) 1 2 0 0 #199# 3670448                  
                          100) (LOGPEN 0 0 10079283) 1 1 4.02228 1.9           
                                0.123113 1)                                    
                          #267=(CLabel                                         
                                (CWidget 0 (0 0) 1 2 0 0 #178# 1944749780 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 43 16 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Bgl}II (1151)" "@N (@S)"
            5 12.1208                                -8.10649 0 -10 2.97739
            0.704328 #266#))                         (CObjectList)))
            (CObjectList))                         #268=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil 3670456 100)                  (CObjectList)
            (CObjectList))                                  #269=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil 0 100)                  
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 1735357044   
            (CStringList) 544370548 2083664691 (CObList))).
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR2'
                     (<a href=""urn:local:.:kr-ezty4ze"">attR2</a>)"
                     /Color="#00cccc"
                     /modified_by="mamee"
     Linker          125..139
                     /modified_by="mamee"
                     /label="GlySer linker"
     CDS             140..691
                     /label="TAP tag"
     primer_bind     200..219
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-TAPtag-L3"
                     /Sequence="AAAATCTCATCCTCCGGGGC"
                     /Tm="59.8"
                     /%GC="55.0"
                     /Hairpin_Tm="34.4"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P41"
     attL3           complement(692..787)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lo-eztz06f"">attL3</a>"
     primer_bind     812..831
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-TAPtag-L3"
                     /Sequence="GGCCGCGATATCCCCTATAG"
                     /Tm="59.3"
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.2"
                     /modified_by="mamee"
                     /label="P40"
     primer          complement(845..865)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        926..976
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    977..1786
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1636..1655
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-TAPtag-L3"
                     /Sequence="CGCAGACCGATACCAGGATC"
                     /Tm="60.0"
                     /%GC="60.0"
                     /Hairpin_Tm="40.0"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P42"
     rep_origin      1933..2521
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     2003..2022
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-TAPtag-L3"
                     /Sequence="TGTTTGCCGGATCAAGAGCT"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.1"
                     /modified_by="mamee"
                     /label="P43"
     misc_feature    2080
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2325..2326
                     /Original_Bases="AT"
                     /label="AT"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2623..2642
                     /#_Off-target_Sites=0
                     /Off-target_Template="pEN-R2-TAPtag-L3"
                     /Sequence="TACCGCCTTTGAGTGAGCTG"
                     /Tm="60.0"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P39"
     primer_bind     3056..3074
                     /Sequence="GATGCCTGGCAGTTCCCTA"
                     /Tm="59.1"
                     /%GC="57.9"
                     /Hairpin_Tm="43.7"
                     /Self_Dimer_Tm="27.2"
                     /created_by="mamee"
                     /label="P46B"
     primer          3120..3140
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
     misc_feature    3204
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtgggtgga ggcggttcaa tggaaaagag aagatggaaa aagaatttca tagccgtctc
      181 agcagccaac cgctttaaga aaatctcatc ctccggggca cttgattatg atattccaac
      241 tactgctagc gagaatttgt attttcaggg tgagctcaaa accgcggctc ttgcgcaaca
      301 cgatgaagcc gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
      361 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
      421 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc
      481 gaaagtagac aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt
      541 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
      601 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt
      661 agacgcgaac tccgcgggga agtcaacctg acaactttat tatacaaagt tggcattata
      721 aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      781 attatttgga gctccatggt agcgttaacg cggccgcgat atcccctata gtgagtcgta
      841 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
      901 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
      961 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     1021 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     1081 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     1141 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     1201 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     1261 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     1321 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     1381 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     1441 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     1501 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     1561 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     1621 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     1681 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     1741 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     1801 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     1861 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     1921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2041 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2581 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2641 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2701 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2761 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
     2821 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     2881 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     2941 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     3001 aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc
     3061 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
     3121 taaaacgacg gccagtctta agctcgggcc ctgcagctct agagctcgaa ttctacaggt
     3181 cactaatacc atctaagtag ttggt
//
