LOCUS       pK7m34GW2-8m21GW3D       15806 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...4y-dv3fmyy
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459373558|                                                 
            VNTDBDATE|459373558|                                               
            LSOWNER|                                                           
            VNTNAME|pK7m34GW2-8m21GW3D,0|                                      
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    31^32
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    64^65
                     /Original_Bases="AC"
                     /label="AC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    68..69
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    136^137
                     /Original_Bases="CTT"
                     /label="CTT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    147^148
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    283..1074
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(728..747)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="1116178714264700ea9a75b0-224d-484b-9447
                     -238426a1fdcc"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P7"
     misc_feature    1114..1117
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1121^1122
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1133^1134
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     rep_origin      1320..1908
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     complement(1500..1519)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="1116178632634999af4e9361-dd22-4387-8020
                     -5b848abcda94"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P6"
     regulatory      complement(1944..2204)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     primer_bind     complement(2324..2343)
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1116178550345700298a5de5-5a73-42b7-8047
                     -bb7b1163ff4f"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P5"
     rep_origin      2578..2772
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2838..3911)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     complement(3126..3143)
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="1116178467799200daa753c6-f7b6-4731-b911
                     -b6d5bdafc3cf"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P4"
     misc_feature    3907
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(3929..3947)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="1116178378270600afb29fe9-82c5-44e1-8df6
                     -cc4ca8e506ca"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P3"
     regulatory      complement(4340..4969)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     complement(4725..4744)
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1116178290180600f431fa24-dd4c-4413-8708
                     -736ead945173"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P2"
     primer_bind     complement(5518..5536)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="1116178207464400c78e4290-e00c-4da9-8a1b
                     -0c72259bccfa"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P1"
     primer_bind     6083..6102
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="11161814404690992b07e099-b1a4-4d61-83d0
                     -440e61d1ddf8"
                     /created_by="primer3"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P37"
     misc_feature    6148..6177
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     T-DNA_border    6185..6385
                     /modified_by="mamee"
                     /label="RB"
     misc_feature    6270
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(6345..6364)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1116179413523400a254f991-7a39-4092-80c5
                     -497b055315b1"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P14"
     misc_feature    6401
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     promoter        6406..6802
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dgycryx"">pROLD</a>"
                     /label="pROLD"
     misc_feature    6413..6414
                     /Original_Bases="TG"
                     /label="TG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6416..6418
                     /Original_Bases="AGG"
                     /label="AGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6420
                     /Original_Bases="CGACTCT"
                     /label="CGACTCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6422
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6424
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6786..6793
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6918
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6983
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     CDS             7022..7753
                     /label="GFP"
     primer_bind     7305..7324
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="26.0"
                     /Tm="60.1"
                     /annotation_group="1116180687183700bce61e8c-ef32-426b-925a
                     -1482b9835b3a"
                     /created_by="primer3"
                     /Sequence="AGGAGCGCACCATCTTCTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P47"
     primer_bind     complement(7521..7540)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="11161804299104009e8c3d4e-fd4d-4829-9392
                     -3b4805e8dc25"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P33"
     terminator      7782..8007
                     /created_by="mamee"
                     /label="t35S"
     misc_feature    7874
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     promoter        8023..8419
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ROLDP
                     (<a href=""urn:local:.:7a-dgycryx"">pROLD</a>)"
                     /modified_by="mamee"
                     /label="pROLD"
     misc_feature    8030..8031
                     /Original_Bases="TG"
                     /label="TG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8033..8035
                     /Original_Bases="AGG"
                     /label="AGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8037
                     /Original_Bases="CGACTCT"
                     /label="CGACTCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8039
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8041
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8403..8410
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     attR1           8426..8550
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        8575..8605
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    8629
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8659..9318
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    9243
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     9321..9340
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.8"
                     /Tm="59.9"
                     /annotation_group="111618198449670085bba0eb-5200-4511-b947
                     -63c2d49c541a"
                     /created_by="primer3"
                     /Sequence="GCGTGGATCCGGCTTACTAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P154"
     UTR             9341..9659
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    9433
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    9651
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             9660..9965
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(10006..10130)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      10142..10908
                     /created_by="mamee"
                     /label="tOCS"
     primer_bind     10160..10179
                     /%GC="55.0"
                     /Hairpin_Tm="38.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="11161807695410997e659e54-9c66-45de-841b
                     -f9c8e7ab4f2a"
                     /created_by="primer3"
                     /Sequence="CGAGACGCCTATGATCGCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P56"
     promoter        10910..11956
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     primer_bind     11093..11113
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="60.0"
                     /annotation_group="111617888080290011d8c6f6-58d2-415a-b517
                     -0ccd1e3e4424"
                     /created_by="primer3"
                     /Sequence="GATGGTTAGAGAGGCCTACGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P10"
     primer_bind     complement(11640..11661)
                     /%GC="45.5"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="12.2"
                     /Tm="59.9"
                     /annotation_group="1116180006332699caeddb0d-b81d-4149-944d
                     -ae509be98337"
                     /created_by="primer3"
                     /Sequence="CGCAATGATGGCATTTGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P18"
     misc_feature    11691
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    11904
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    11980^11981
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     primer          12001..12017
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     attR4           12037..12160
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(12201..12506)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(12507..12825)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.06%"
                     /Primary_Match="99.06%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    12580
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    12659
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(12833..12853)
                     /%GC="57.1"
                     /Hairpin_Tm="33.7"
                     /Self_Dimer_Tm="6.0"
                     /Tm="60.1"
                     /annotation_group="1116182978047099798e1e8a-8fea-4655-ab6f
                     -61b399196a51"
                     /created_by="primer3"
                     /Sequence="GCGTAATCTAGAGGATCCGGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P166"
     misc_feature    complement(12848..13506)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    12857^12858
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     promoter        complement(13560..13590)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    13594^13595
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR3           complement(13614..13738)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     primer          complement(13747..13762)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     primer          complement(13766..13782)
                     /invitrogen=2000000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-40) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-40) forward primer (pDONRP4-P3 - M13 (-40) forward
                     primer)"
                     /label="M13 (-40) forward primer"
     misc_feature    13788^13789
                     /Original_Bases="GCGGCCATGGCGGCCGGGA"
                     /label="GCGGCCATGGCGGCCGGGA"
                     /note="Geneious type: Editing History Deletion"
     terminator      13790..14015
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     misc_feature    14023^14024
                     /Original_Bases="GCGACGTCG"
                     /label="GCGACGTCG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14056^14057
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14059
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    14069..14071
                     /Original_Bases="ATA"
                     /label="ATA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    14075..14079
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        14080..14352
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     Plant_Selection 14080..15453
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     misc_feature    14177
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             14365..15159
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     complement(14404..14423)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="1116180180588200604a1e61-47f0-4374-b119
                     -deac1aadc17f"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P25"
     primer_bind     complement(15017..15036)
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="11161800950730009df07c0d-9a3a-499f-95cc
                     -f974ac3318d5"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P24"
     terminator      15186..15453
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     T-DNA_border    15474..15806
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     complement(15718..15737)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="1116178797237900f3e53441-3b74-4a4c-a549
                     -7b284e85ed4c"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3D"
                     /label="P8"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
      301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
     6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     6361 atctgatcca agctcaagct aagcttgcat gcctgcaggt cagcttgcat gcccccacta
     6421 caatgaattt gttcgtgaac tattagttgc gggccttggc atccgactac ctctgcggca
     6481 atattatatt ccctgggccc accgtgaacc caatttcgcc tatttattca ttacccccat
     6541 taacattgaa gtagtcatga tgggcctgca gcacgttggt gaggctggca caactcatcc
     6601 atatactttc tgaccggatc ggcacattat tgtagaaaac gcggacccac agcgcacttt
     6661 ccaaagcggt gccgcgtcag aatgcgctgg cagaaaaaaa ttaatccaaa agtaccctcc
     6721 aagcagccca tataaacgcg tttacaaatc cgctaacctc aacaatttga gcagagaaaa
     6781 ttcgcaccta caatagagga tccccgggta ccgagctcga attctcaaca caacatatac
     6841 aaaacaaacg aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt
     6901 tcttttaaag caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tagccatggt
     6961 gaagactaat ctttttctct ttctcatctt ttcacttctc ctatcattat cctcggccga
     7021 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     7081 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7141 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     7201 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     7261 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     7321 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     7381 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     7441 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     7501 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     7561 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     7621 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     7681 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagaa
     7741 ggacgagctg taagcgccgc ccggctgcag cccgggggat ccactagttc tagagtccgc
     7801 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     7861 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     7921 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     7981 attcctaaaa ccaaaatcca gtgacctgca ggcatgcaag cttgcatgcc cccactacaa
     8041 tgaatttgtt cgtgaactat tagttgcggg ccttggcatc cgactacctc tgcggcaata
     8101 ttatattccc tgggcccacc gtgaacccaa tttcgcctat ttattcatta cccccattaa
     8161 cattgaagta gtcatgatgg gcctgcagca cgttggtgag gctggcacaa ctcatccata
     8221 tactttctga ccggatcggc acattattgt agaaaacgcg gacccacagc gcactttcca
     8281 aagcggtgcc gcgtcagaat gcgctggcag aaaaaaatta atccaaaagt accctccaag
     8341 cagcccatat aaacgcgttt acaaatccgc taacctcaac aatttgagca gagaaaattc
     8401 gcacctacaa tagaggatcc ccatcacaag tttgtacaaa aaagctgaac gagaaacgta
     8461 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     8521 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     8581 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     8641 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     8701 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     8761 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     8821 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     8881 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     8941 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     9001 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     9061 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     9121 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     9181 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     9241 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     9301 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     9361 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     9421 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     9481 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     9541 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     9601 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     9661 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     9721 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     9781 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     9841 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     9901 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     9961 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
    10021 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
    10081 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gatctagagt
    10141 cctgctttaa tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt
    10201 tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt
    10261 cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca
    10321 atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt
    10381 gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt
    10441 tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat
    10501 tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga
    10561 gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg
    10621 agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa
    10681 cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt
    10741 tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg
    10801 ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg acctgcaggc
    10861 atgcaagcta gcttactagt gcggccgcct gcaggtcgac catatgggag agctctccca
    10921 tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc
    10981 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
    11041 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
    11101 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
    11161 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
    11221 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
    11281 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
    11341 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
    11401 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
    11461 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    11521 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    11581 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    11641 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca
    11701 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    11761 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
    11821 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
    11881 ggactccggt atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat
    11941 ttactattct agtcgacctg caggcggccg cactagtgat gagcggataa caatttcaca
    12001 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgaac
    12061 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
    12121 ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc agactggctg
    12181 tgtataaggg agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
    12241 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
    12301 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
    12361 tcacggggga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
    12421 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
    12481 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc
    12541 gttcatttca ataaaccggg cgacctcagc catcccttct tgattttccg ctttccagcg
    12601 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccgaac cggagatact
    12661 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
    12721 tgcttcatag catacctctt tttgacatac ttcgggtata catatcagta tatattctta
    12781 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
    12841 ctctagatta cgccccgcct gccactcatc gcagtactgt tgtaattcat taagcattct
    12901 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
    12961 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
    13021 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
    13081 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
    13141 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
    13201 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
    13261 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
    13321 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
    13381 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
    13441 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
    13501 ctccatttta gcttccttag ctcctgaaaa tctcgacgga tcctaactca aaatccacac
    13561 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgcgg ccgccatagt
    13621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
    13681 aatatattga tatttatatc attttacgtt tctcgttcaa ctttattata catagttgat
    13741 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa acgatcccgc ggccatgcta
    13801 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
    13861 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
    13921 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    13981 aatttctaat tcctaaaacc aaaatccagt gacctgcagg catgcgacgt cgggccctct
    14041 agaggatccc cgggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt
    14101 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa
    14161 cgttgaagga gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca
    14221 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct
    14281 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat
    14341 attcactctc aactcgatcg aggcatgatt gaacaagatg gattgcacgc aggttctccg
    14401 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    14461 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    14521 ctgtccggtg ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg
    14581 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    14641 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    14701 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    14761 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    14821 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    14881 aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccacgg cgatgcctgc
    14941 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    15001 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    15061 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    15121 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggac
    15181 tctagctaga gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
    15241 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
    15301 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
    15361 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
    15421 tcgcgcgcgg tgtcatctat gttactagat cgaccggcat gcaagctgat aattcaattc
    15481 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
    15541 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
    15601 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
    15661 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
    15721 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
    15781 tgacagagcg ttgctgcctg tgatca
//
