LOCUS       pK7m34GW2-8m21GW3       14169 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...1i-dv38wfg
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|459373309|                                                 
            VNTDBDATE|459373309|                                               
            LSOWNER|                                                           
            VNTNAME|pK7m34GW2-8m21GW3,0|                                       
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                       (CObjectList) (CObjectList))) (CObjectList)))        
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            16711680    (CStringList) 2081903016 0 (CObList))).
FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    31^32
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    64^65
                     /Original_Bases="AC"
                     /label="AC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    68..69
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    136^137
                     /Original_Bases="CTT"
                     /label="CTT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    147^148
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    283..1074
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(728..747)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="1115738143464800206ab546-0783-4082-b034
                     -1b769e8c4655"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P7"
     misc_feature    1114..1117
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1121^1122
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1133^1134
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     rep_origin      1320..1908
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     complement(1500..1519)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="11157380286874001117f31e-696b-4bf8-9a2e
                     -fe5587b963c6"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P6"
     regulatory      complement(1944..2204)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     primer_bind     complement(2324..2343)
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1115737943654700540720de-8682-4ad6-842e
                     -d5cbf074cfd9"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P5"
     rep_origin      2578..2772
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2838..3911)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     complement(3126..3143)
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="1115737860016300ad1644a1-a262-4f1c-aa56
                     -9eacf0977d44"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P4"
     misc_feature    3907
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(3929..3947)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="1115737776238699ad0b1483-295a-4c24-81c8
                     -31f95f1f4b97"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P3"
     regulatory      complement(4340..4969)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     complement(4725..4744)
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="111573769353150044eb4130-9f5e-419b-b538
                     -e60f7ca8051b"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P2"
     primer_bind     complement(5518..5536)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="111573761011220002cc7759-49b6-40a9-a388
                     -7f2ae38c395f"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P1"
     primer_bind     6083..6102
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="111574036543100037b0ea1d-a913-4658-a891
                     -cdfd09f5ca83"
                     /created_by="primer3"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P37"
     misc_feature    6148..6177
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     T-DNA_border    6185..6385
                     /modified_by="mamee"
                     /label="RB"
     misc_feature    6270
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(6345..6364)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1115738865152000c76b77ca-6737-44bd-818d
                     -c3aead9ea9b2"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P14"
     promoter        6386..6782
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dgycryx"">pROLD</a>"
                     /label="pROLD"
     misc_feature    6393..6394
                     /Original_Bases="TG"
                     /label="TG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6396..6398
                     /Original_Bases="AGG"
                     /label="AGG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6400
                     /Original_Bases="CGACTCT"
                     /label="CGACTCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6402
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6404
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    6766..6773
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     attR1           6789..6913
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        6938..6968
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    6992
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    7022..7681
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    7606
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     7684..7703
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.8"
                     /Tm="59.9"
                     /annotation_group="1115740858318200b29218c9-25cb-4b76-84ea
                     -91775136ae61"
                     /created_by="primer3"
                     /Sequence="GCGTGGATCCGGCTTACTAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P154"
     UTR             7704..8022
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    7796
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8014
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             8023..8328
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(8369..8493)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      8505..9271
                     /created_by="mamee"
                     /label="tOCS"
     primer_bind     8523..8542
                     /%GC="55.0"
                     /Hairpin_Tm="38.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="111573969180540016568cb6-af96-4ae4-a467
                     -952b02e57181"
                     /created_by="primer3"
                     /Sequence="CGAGACGCCTATGATCGCAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P56"
     promoter        9273..10319
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     primer_bind     complement(9708..9729)
                     /%GC="45.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.0"
                     /Tm="60.0"
                     /annotation_group="11157406954495001bcc4e94-da0f-4dd9-8468
                     -39e8f186e331"
                     /created_by="primer3"
                     /Sequence="TGACTCCATGGCCTTTGATTCA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P151"
     misc_feature    10054
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    10267
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    10343^10344
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     primer          10364..10380
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     primer_bind     complement(10388..10408)
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.5"
                     /annotation_group="11157417683452005018bc6f-5e58-4456-ae0f
                     -fe749303710b"
                     /created_by="primer3"
                     /Sequence="ACAAAGTTGATAGCTTGGCGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P165"
     attR4           10400..10523
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(10564..10869)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(10870..11188)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.06%"
                     /Primary_Match="99.06%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    10943
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    11022
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(11196..11216)
                     /%GC="57.1"
                     /Hairpin_Tm="33.7"
                     /Self_Dimer_Tm="6.0"
                     /Tm="60.1"
                     /annotation_group="11157418527575002b666ac9-616e-49ac-aff3
                     -5b5b6cfa9a58"
                     /created_by="primer3"
                     /Sequence="GCGTAATCTAGAGGATCCGGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P166"
     misc_feature    complement(11211..11869)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    11220^11221
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     promoter        complement(11923..11953)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    11957^11958
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR3           complement(11977..12101)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     primer          complement(12110..12125)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     misc_feature    12151^12152
                     /Original_Bases="GCGGCCATGGCGGCCGGGA"
                     /label="GCGGCCATGGCGGCCGGGA"
                     /note="Geneious type: Editing History Deletion"
     terminator      12153..12378
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     complement(12228..12251)
                     /%GC="45.8"
                     /Hairpin_Tm="32.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="1115740033719900d0489986-0052-43a1-80b3
                     -530baadff869"
                     /created_by="primer3"
                     /Sequence="CCTTATCTGGGAACTACTCACACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P65"
     misc_feature    12386^12387
                     /Original_Bases="GCGACGTCG"
                     /label="GCGACGTCG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    12419^12420
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    12422
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    12432..12434
                     /Original_Bases="ATA"
                     /label="ATA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    12438..12442
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        12443..12715
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     Plant_Selection 12443..13816
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     misc_feature    12540
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     CDS             12728..13522
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     complement(12767..12786)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="11157395257796994ab1eaf3-a3e5-4d1e-be40
                     -4b8d8718625f"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P25"
     primer_bind     complement(13380..13399)
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="11157394443420002166b2ae-7eb7-4199-8b1c
                     -418d5f79d882"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P24"
     terminator      13549..13816
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     T-DNA_border    13837..14169
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     complement(14081..14100)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="11157382532522000cbcf3f7-2434-4646-88cc
                     -44fcae5cf750"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pK7m34GW2-8m21GW3"
                     /label="P8"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
      301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
     6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     6361 atctgatcca agctcaagct aagcttgcat gcccccacta caatgaattt gttcgtgaac
     6421 tattagttgc gggccttggc atccgactac ctctgcggca atattatatt ccctgggccc
     6481 accgtgaacc caatttcgcc tatttattca ttacccccat taacattgaa gtagtcatga
     6541 tgggcctgca gcacgttggt gaggctggca caactcatcc atatactttc tgaccggatc
     6601 ggcacattat tgtagaaaac gcggacccac agcgcacttt ccaaagcggt gccgcgtcag
     6661 aatgcgctgg cagaaaaaaa ttaatccaaa agtaccctcc aagcagccca tataaacgcg
     6721 tttacaaatc cgctaacctc aacaatttga gcagagaaaa ttcgcaccta caatagagga
     6781 tccccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     6841 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     6901 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     6961 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     7021 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     7081 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     7141 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     7201 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     7261 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     7321 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     7381 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     7441 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     7501 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     7561 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     7621 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     7681 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     7741 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     7801 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     7861 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     7921 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     7981 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     8041 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     8101 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     8161 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     8221 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     8281 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     8341 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     8401 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     8461 cgtttctcgt tcagctttct tgtacaaagt ggtgatctag agtcctgctt taatgagata
     8521 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     8581 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     8641 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc
     8701 tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag
     8761 cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa
     8821 ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca
     8881 aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg
     8941 ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga
     9001 gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg
     9061 gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc
     9121 ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc
     9181 gaattttgcg acaacatgtc gaggctcagc aggacctgca ggcatgcaag ctagcttact
     9241 agtgcggccg cctgcaggtc gaccatatgg gagagctctc ccatatggtc gactagagcc
     9301 aagctgatct cctttgcccc ggagatcacc atggacgact ttctctatct ctacgatcta
     9361 ggaagaaagt tcgacggaga aggtgacgat accatgttca ccaccgataa tgagaagatt
     9421 agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc ctacgcggca
     9481 ggtctcatca agacgatcta cccgagtaat aatctccagg agatcaaata ccttcccaag
     9541 aaggttaaag atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat
     9601 atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt gcttcataaa
     9661 ccaaggcaag taatagagat tggagtctct aagaaagtag ttcctactga atcaaaggcc
     9721 atggagtcaa aaattcagat cgaggatcta acagaactcg ccgtgaagac tggcgaacag
     9781 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     9841 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     9901 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     9961 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
    10021 tgcgataaag gaaaggctat cgttcaagat gcccctgccg acagtggtcc caaagatgga
    10081 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
    10141 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
    10201 caagaccctt cctctatata aggaagttca tttcatttgg agaggactcc ggtattttta
    10261 caacaattac cacaacaaaa caaacaacaa acaacattac aatttactat tctagtcgac
    10321 ctgcaggcgg ccgcactagt gatgagcgga taacaatttc acacaggaaa cagctatgac
    10381 catgattacg ccaagctatc aactttgtat agaaaagttg aacgagaaac gtaaaatgat
    10441 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
    10501 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
    10561 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
    10621 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
    10681 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
    10741 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
    10801 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
    10861 aaactgcatt tcaccagccc ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc
    10921 gggcgacctc agccatccct tcttgatttt ccgctttcca gcgttcggca cgcagacgac
    10981 gggcttcatt ctgcatggtt gtgcttaccg aaccggagat actgacatca tatatgcctt
    11041 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
    11101 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
    11161 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
    11221 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
    11281 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
    11341 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
    11401 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
    11461 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
    11521 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
    11581 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
    11641 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
    11701 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
    11761 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
    11821 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
    11881 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
    11941 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
    12001 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
    12061 atcattttac gtttctcgtt caactttatt atacatagtt gataattcac tggccgtcgt
    12121 tttacaacgt cgtgactggg aaaacgatcc cgcggccatg ctagagtccg caaaaatcac
    12181 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
    12241 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
    12301 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
    12361 accaaaatcc agtgacctgc aggcatgcga cgtcgggccc tctagaggat ccccgggtac
    12421 cgcgaattat cgatcatgag cggagaatta agggagtcac gttatgaccc ccgccgatga
    12481 cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa ggagccactg
    12541 agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca ttattgcgcg
    12601 ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa aatgctccac
    12661 tgacgttcca taaattcccc tcggtatcca attagagtct catattcact ctcaactcga
    12721 tcgaggcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    12781 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    12841 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    12901 tgaactccaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
    12961 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    13021 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    13081 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    13141 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    13201 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat
    13261 gcccgacggc gaggatctcg tcgtgaccca cggcgatgcc tgcttgccga atatcatggt
    13321 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    13381 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    13441 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    13501 ccttcttgac gagttcttct gagcgggact ctggggttcg gactctagct agagtcaagc
    13561 agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
    13621 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
    13681 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
    13741 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
    13801 tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta attcagtaca
    13861 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
    13921 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
    13981 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
    14041 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
    14101 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
    14161 ctgtgatca
//
