LOCUS       pKNTAP                 14155 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...ny-duywt50
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|451225192|                                                 
            VNTDBDATE|457772991|                                               
            LSOWNER|                                                           
            VNTNAME|pKNTAP|                                                    
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Mansour Karimi|                                     
            VNTOAUTHORTEL|+3193313850|                                         
            VNTOAUTHOREML|mansour.karimi@psb.ugent.be|                         
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            17 0 0 400 255 1 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Xho}I (10454)" "@N (@S)" 5 -14.749                           
                -1.15807 0 -10 3.44446 0.694805 #358#))                        
            (CObjectList))                                               
            #360=(CGroupWidget                                                 
                  (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -1212 100)                 
                   (CObjectList                                                
                    #361=(CScratch                                             
                          (CWidget 1 (1 #6# 1) 1 2 0 0 #230# 673 100)          
                                 (LOGPEN 0 10 0) 8 1 2.68273 1.9 0.123113 1)   
                                  #362=(CLabel                                 
                                        (CWidget 0 (0 0) 1 2 0 0 #271# 28918004
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Apa}I
            (8082)" "@N (@S)" 5 -8.11097                               
            -11.5807 0 -10 3.11923 0.694805 #361#)                         
            #363=(CScratch                                                     
                  (CWidget 2 (1 #6# 2) 1 2 0 0 #230# 0 100)                    
                       (LOGPEN 0 10 0) 8 1 0.692232 1.9 0.123113 1)            
                         #364=(CLabel                                          
                               (CWidget 0 (0 0) 1 2 0 0 #271# 40489052 100)    
                                       (LOGPEN 0 0 8437824) 1                  
                                       (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Apa}I (12562)" "@N (@S)" 5
            -10.7354                                11.5807 0 -10 3.44446
            0.694805 #363#))                         (CObjectList))            
                                               #365=(CGroupWidget              
                                                     (CWidget 2 (10 #13# 0) 1 2
            0 0 Nil -979 100)                         (CObjectList             
                                                       #366=(CScratch          
                                                             (CWidget 1 (1 #13#
            1) 1 2 0 0 #230# 35271564                                 100)
            (LOGPEN 0 10 0) 8 1 0.74466 1.9                               
            0.123113 1)                                                  
            #367=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #271# 40968772 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Hin}dIII (12444)" "@N (@S)" 5 -11.4303    
                                       10.4226 0 -10 3.99143 0.694805 #366#)   
                                  #368=(CScratch                               
                                        (CWidget 2 (1 #13# 2) 1 2 0 0 #230# 0
            100)                                (LOGPEN 0 10 0) 8 1 0.0208825
            1.9 0.123113 1)                          #369=(CLabel              
                                                           (CWidget 0 (0 0) 1 2
            0 0 #271# 18022976 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Hin}dIII (14073)" "@N (@S)" 5 -3.3941                        
                   18.5291 0 -10 3.99143 0.694805 #368#))                      
              (CObjectList))                                               
            #370=(CGroupWidget                                                 
                  (CWidget 2 (10 #15# 0) 1 2 0 0 Nil -1371 100)                
                    (CObjectList                                               
                     #371=(CScratch                                            
                           (CWidget 1 (1 #15# 1) 1 2 0 0 #230# 875 100)        
                                   (LOGPEN 0 10 0) 8 1 5.5303 1.9 0.123113 1)  
                                   #372=(CLabel                                
                                         (CWidget 0 (0 0) 1 2 0 0 #271#
            41083180 100)                                (LOGPEN 0 0 8437824) 1
                                                         (LOGFONT 47 17 0 0 400
            255 0 0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Nde}I
            (1673)" "@N (@S)" 5 11.1972                                9.26456
            0 -10 3.16358 0.694805 #371#)                         
            #373=(CScratch                                                     
                  (CWidget 2 (1 #15# 2) 1 2 0 0 #230# 29037356                 
                           100) (LOGPEN 0 10 0) 8 1 4.42442 1.9                
                           0.123113 1)                                         
                     #374=(CLabel                                              
                           (CWidget 0 (0 0) 1 2 0 0 #271# 268786568 100)       
                                    (LOGPEN 0 0 8437824) 1                     
                                    (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34  
                                          "Arial") 1.15807 0.611111 0          
                                         "{\\i Nde}I (4162)" "@N (@S)" 5
            14.2253                                -3.47421 0 -10 3.16358
            0.694805 #373#))                         (CObjectList))            
                                               #375=(CGroupWidget              
                                                     (CWidget 2 (10 #19# 0) 1 2
            0 0 Nil -1763 100)                         (CObjectList            
                                                        #376=(CScratch         
                                                              (CWidget 1 (1
            #19# 1) 1 2 0 0 #230# 28512320                                 100)
            (LOGPEN 0 10 0) 8 1 0.855737 1.9                               
            0.123113 1)                                                  
            #377=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #271# 18023048 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Sac}I (12194)" "@N (@S)" 5 -12.3003       
                                    8.10649 0 -10 3.40011 0.694805 #376#)      
                               #378=(CScratch                                  
                                     (CWidget 2 (1 #19# 2) 1 2 0 0 #230# 393227
                                            100) (LOGPEN 0 10 0) 8 1 0.554496
            1.9                                0.123113 1)                     
                                         #379=(CLabel                          
                                               (CWidget 0 (0 0) 1 2 0 0 #271#
            29441796 100)                                (LOGPEN 0 0 8437824) 1
                                                         (LOGFONT 47 17 0 0 400
            255 0 0 0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sac}I
            (12872)" "@N (@S)" 5 -9.51651                               
            15.0549 0 -10 3.40011 0.694805 #378#))                        
            (CObjectList))                                               
            #380=(CGroupWidget                                                 
                  (CWidget 2 (10 #20# 0) 1 2 0 0 Nil -2076 100)                
                    (CObjectList                                               
                     #381=(CScratch                                            
                           (CWidget 1 (1 #20# 1) 1 2 0 0 #230# 34051140        
                                    100) (LOGPEN 0 10 0) 8 1 2.74716 1.9       
                                    0.123113 1)                                
                              #382=(CLabel                                     
                                    (CWidget 0 (0 0) 1 2 0 0 #271# 40951364
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sac}II
            (7937)" "@N (@S)" 5 -7.45914                               
            -12.7388 0 -10 3.26706 0.694805 #381#)                         
            #383=(CScratch                                                     
                  (CWidget 2 (1 #20# 2) 1 2 0 0 #230# 34536336                 
                           100) (LOGPEN 0 10 0) 8 1 0.847295 1.9               
                            0.123113 1)                                        
                      #384=(CLabel (CWidget 0 (0 0) 1 2 0 0 #271# 0 100)       
                                    (LOGPEN 0 0 8437824) 1                     
                                    (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34  
                                          "Arial") 1.15807 0.611111 0          
                                         "{\\i Sac}II (12213)" "@N (@S)" 5
            -12.0891                                9.26456 0 -10 3.59229
            0.694805 #383#))                         (CObjectList)))
            (CObjectList))                         #385=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -196 100)                  (CObjectList)
            (CObjectList))                                  #386=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -1682 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 68092089   
            (CStringList) 0 327734 (CObList))).
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="110700067438480022c9b563-3151-498e-a61f
                     -d22072107959"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     771..790
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="1107001645437600f0ac9a19-39d1-4157-9b4b
                     -4a5e27068e4a"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P24"
     primer_bind     1114..1133
                     /%GC="55.0"
                     /Hairpin_Tm="41.6"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="11070032538301000a2e265a-bc48-415d-8cb8
                     -b6cbc5d8fe10"
                     /created_by="primer3"
                     /Sequence="GCAGGAGCAAGGTGAGATGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P158"
     primer_bind     complement(1294..1313)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.6"
                     /annotation_group="11070031720596994e2bfd26-1433-4c43-bbe5
                     -a6dd25b7399c"
                     /created_by="primer3"
                     /Sequence="CGCCCGGTTCTTTTTGTCAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P157"
     primer_bind     1384..1403
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="1107001728005900e97fd041-4f4c-4bd3-9fd4
                     -e4fe760875f2"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P25"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1728..1732
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1748
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1750^1751
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1769^1770
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     primer          1783..1799
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     primer_bind     complement(1807..1827)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="ACAAAGTTGATAGCTTGGCGT"
                     /Tm="58.5"
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P165"
     attR4           1819..1942
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(1983..2288)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(2289..2607)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.06%"
                     /Primary_Match="99.06%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2362
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2441
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(2615..2635)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="GCGTAATCTAGAGGATCCGGC"
                     /Tm="60.1"
                     /%GC="57.1"
                     /Hairpin_Tm="33.7"
                     /Self_Dimer_Tm="6.0"
                     /modified_by="mamee"
                     /label="P166"
     misc_feature    complement(2630..3288)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    2641^2642
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     promoter        complement(3342..3372)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    3380^3381
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR3           complement(3396..3520)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     primer          complement(3529..3544)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     RBS             3553..3584
                     /label="omega leader"
     misc_difference 3585..4127
                     /label="NTAPi"
     primer_bind     3668..3687
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="ACGCAATGCCTTCATCCAGA"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="31.3"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P167"
     primer_bind     complement(3833..3852)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="TACGCTGTTCCTCGTTCAGG"
                     /Tm="59.8"
                     /%GC="55.0"
                     /Hairpin_Tm="45.2"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P168"
     primer_bind     4049..4068
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="GAGCAGCAGATGGAAGAGCA"
                     /Tm="60.1"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P169"
     attR1           4152..4276
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        4301..4331
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    4355
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4385..5044
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    4969
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     5047..5066
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.8"
                     /Tm="59.9"
                     /annotation_group="1107002913519700811bbff4-ce49-4df4-bae1
                     -50c12f8d5c92"
                     /created_by="primer3"
                     /Sequence="GCGTGGATCCGGCTTACTAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P154"
     UTR             5067..5385
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    5159
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5219
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    5377
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             5386..5691
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(5732..5856)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      5902..6127
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     6157..6176
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="16.7"
                     /Tm="60.0"
                     /annotation_group="1107002057919899c71941d3-52ad-4020-bd91
                     -1c23058b045d"
                     /created_by="primer3"
                     /Sequence="TTGCATGCCCCCACTACAAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P38"
     promoter        6158..6554
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dgycryx"">pROLD</a>"
                     /label="pROLD"
     misc_feature    6538..6545
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    6670
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(6695..6714)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /Sequence="TCACCATGGCTATCGTTCGT"
                     /Tm="59.2"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.0"
                     /modified_by="mamee"
                     /label="P170"
     CDS             6708..7505
                     /label="EgfpER"
     misc_feature    6735
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     6954..6973
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="1107001809932899d889b529-314c-47ae-8ac3
                     -5a82e044e9b3"
                     /created_by="primer3"
                     /Sequence="CTCGTGACCACCCTGACCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P27"
     terminator      7546..7770
                     /modified_by="mamee"
                     /label="t35S"
     misc_feature    7626
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     T-DNA_border    7771..7971
                     /modified_by="mamee"
                     /label="RB"
     primer_bind     7792..7811
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="110700112241510073d13e65-ea01-4607-8e4b
                     -a21a4a1fcd14"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P14"
     misc_feature    7886
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    7979..8008
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     8620..8638
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="1107000092175100ef334463-dec9-4383-8d69
                     -dc4110a510b0"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P1"
     regulatory      9187..9816
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     9412..9431
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="11070001761667009fe54ac1-7d54-48c9-bd66
                     -d3ac00b24cbf"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P2"
     primer_bind     10209..10227
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="1107000258722100c416f8e8-d1b5-4ade-9529
                     -2fbeda58d7d6"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P3"
     regulatory      10245..11318
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    10249
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     11013..11030
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="11070003420219002107185c-15f4-4f85-a7c2
                     -d9ff9304db4d"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P4"
     rep_origin      11384..11578
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     11813..11832
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1107000426988800c2c31788-da7d-41ec-bd86
                     -58a895409757"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P5"
     regulatory      11952..12212
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(12248..12836)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     12637..12656
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="1107000508711000f9a18f76-d991-4deb-99fa
                     -5a47cb95bed9"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P6"
     misc_feature    13022^13023
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    13034^13035
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    13039..13042
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(13082..13873)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     13409..13428
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="1107000591684800a39e52e0-9982-4305-bdc1
                     -41d3718d593b"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKNTAP"
                     /label="P7"
     Promoter        complement(13874..14148)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    14008^14009
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14019^14020
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14087..14088
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    14091^14092
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    14124^14125
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgcgg tacccgggga tcctctagag gcccggcgcg cccaggaaac agctatgacc
     1801 atgattacgc caagctatca actttgtata gaaaagttga acgagaaacg taaaatgata
     1861 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     1921 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac
     1981 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg
     2041 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc
     2101 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacgggg gactttatct
     2161 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata
     2221 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
     2281 aactgcattt caccagcccc tgttctcgtc ggcaaaagag ccgttcattt caataaaccg
     2341 ggcgacctca gccatccctt cttgattttc cgctttccag cgttcggcac gcagacgacg
     2401 ggcttcattc tgcatggttg tgcttaccga accggagata ctgacatcat atatgccttg
     2461 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
     2521 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
     2581 gcaaatacgc atactgttat ctggctttta gtaagccgga tcctctagat tacgccccgc
     2641 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2701 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2761 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2821 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     2881 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2941 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     3001 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     3061 tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     3121 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     3181 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     3241 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     3301 agctcctgaa aatctcgacg gatcctaact caaaatccac acattatacg agccggaagc
     3361 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
     3421 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3481 tcattttacg tttctcgttc aactttatta tacatagttg ataattcact ggccgtcgtt
     3541 ttacttaatt aaaacaacaa acaacaaaca acattacaat tacaatggtg gtcgacaaca
     3601 agttcaacaa ggaacagcaa aacgcgttct acgagatcct gcacctgccg aacctcaacg
     3661 aagagcaacg caatgccttc atccagagcc tgaaggacga tccgtcccag agcgccaacc
     3721 tgctcgccga agcgaagaag ctgaacgacg cgcaggcacc gaagatgcat gtggacaaca
     3781 agttcaacaa ggagcaacag aacgccttct acgagatcct ccatctccca aacctgaacg
     3841 aggaacagcg taacgcgttc atccagtccc tcaaggatga cccaagccaa tccgcgaacc
     3901 tcctggcgga ggccaagaag ctcaacgatg cccaagcccc aaaggactac gacatcccaa
     3961 ccactgccag cgagaacctg tacttccagg gcgagctgaa gaccgcagct ctggcacagc
     4021 acgacgacgt gggcagcact agtatggaga gcagcagatg gaagagcaac ttcatcgcag
     4081 tgagcgccgc aaaccgcttc aagaagatca gctccagcgg cgcactgggt ggaggcggtt
     4141 cactcgagat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     4201 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     4261 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     4321 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     4381 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     4441 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     4501 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     4561 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     4621 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     4681 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     4741 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     4801 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     4861 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     4921 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     4981 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     5041 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     5101 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     5161 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggaa
     5221 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     5281 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     5341 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     5401 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     5461 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     5521 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     5581 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     5641 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     5701 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     5761 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     5821 ttacgtttct cgttcagctt tcttgtacaa agtggtgata tcactagtgc ggccgcctga
     5881 gctcatctaa ctgactagcc gcggccatgc tagagtccgc aaaaatcacc agtctctctc
     5941 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     6001 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     6061 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     6121 gtgacctgca ggaagcttgc atgcctgcag gtcagcttgc atgcccccac tacaatgaat
     6181 ttgttcgtga actattagtt gcgggccttg gcatccgact acctctgcgg caatattata
     6241 ttccctgggc ccaccgtgaa cccaatttcg cctatttatt cattaccccc attaacattg
     6301 aagtagtcat gatgggcctg cagcacgttg gtgaggctgg cacaactcat ccatatactt
     6361 tctgaccgga tcggcacatt attgtagaaa acgcggaccc acagcgcact ttccaaagcg
     6421 gtgccgcgtc agaatgcgct ggcagaaaaa aattaatcca aaagtaccct ccaagcagcc
     6481 catataaacg cgtttacaaa tccgctaacc tcaacaattt gagcagagaa aattcgcacc
     6541 tacaatagag gatccccggg taccgagctc gaattctcaa cacaacatat acaaaacaaa
     6601 cgaatctcaa gcaatcaagc attctacttc tattgcagca atttaaatca tttcttttaa
     6661 agcaaaagca attttctgaa aattttcacc atttacgaac gatagccatg gtgaagacta
     6721 atctttttct ctttctcatc ttttcacttc tcctatcatt atcctcggcc gacatggtga
     6781 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6841 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     6901 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6961 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     7021 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7081 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7141 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7201 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     7261 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     7321 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     7381 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     7441 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aaggacgagc
     7501 tgtaagcgcc gcccggctgc agcccggggg atccactagt tctagagtcc gcaaaaatca
     7561 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     7621 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     7681 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     7741 aaccaaaatc cagtgacctg caggcatgca agcttagctt gagcttggat cagattgtcg
     7801 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     7861 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     7921 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttaa
     7981 agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     8041 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     8101 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     8161 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     8221 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     8281 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     8341 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     8401 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     8461 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     8521 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     8581 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     8641 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     8701 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     8761 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     8821 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     8881 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     8941 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     9001 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     9061 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     9121 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     9181 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     9241 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     9301 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     9361 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     9421 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     9481 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     9541 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     9601 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     9661 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     9721 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     9781 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     9841 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     9901 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9961 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    10021 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    10081 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    10141 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
    10201 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
    10261 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
    10321 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    10381 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
    10441 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    10501 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    10561 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    10621 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    10681 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    10741 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    10801 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    10861 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    10921 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    10981 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    11041 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    11101 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    11161 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    11221 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    11281 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    11341 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
    11401 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    11461 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    11521 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    11581 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    11641 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    11701 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    11761 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    11821 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11881 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    11941 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    12001 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    12061 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    12121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    12361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    12421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    12481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    12541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    12601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    12661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    12721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    12781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12901 ttttggtcat gcatgatata tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa
    12961 taataaaagc agacttgacc tgatagtttg gctgtgagca attatgtgct tagtgcatct
    13021 aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattt ctagctagac
    13081 attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
    13141 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
    13201 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
    13261 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
    13321 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
    13381 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
    13441 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
    13501 ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
    13561 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
    13621 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
    13681 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
    13741 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
    13801 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
    13861 ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
    13921 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
    13981 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
    14041 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
    14101 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc cgaat
//
