LOCUS       pKCTAP                 11854 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...q2-dozbnly
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is best viewed with a free Vector NTI Viewer:            
            http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|450972769|                                                 
            VNTDBDATE|457853382|                                               
            LSOWNER|                                                           
            VNTNAME|pKCTAP|                                                    
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Mansour Karimi|                                     
            VNTOAUTHORTEL|+3193313850|                                         
            VNTOAUTHOREML|mansour.karimi@psb.ugent.be|                         
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FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="59925514202258009dff83c2-3940-4c35-a376
                     -ce4769bbd922"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     misc_feature    461
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     771..790
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="5992551795626700f40c1a99-6abb-4473-a09c
                     -69d5be47bc2f"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P24"
     misc_feature    879
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1012
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     1384..1403
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="59925518530082001a889f64-f918-4487-a76c
                     -9d35e0845e20"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P25"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1728..1732
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1776..1795
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer          1796..1812
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     attR4           1832..1955
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     CDS             complement(1996..2301)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     primer_bind     2256..2276
                     /%GC="47.6"
                     /Hairpin_Tm="45.0"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /annotation_group="59925516479322006f9a13b0-c236-42cd-adcf
                     -a52d62537348"
                     /created_by="primer3"
                     /Sequence="GACGATAACGGCTCTCTCTTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P15"
     UTR             complement(2302..2620)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="98.43%"
                     /Primary_Match="98.43%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2375
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2454
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    complement(2643..3301)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    2654^2655
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     primer_bind     3052..3071
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59925516812738004b105c74-14d7-4e9a-8281
                     -4d873eee3f7e"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P16"
     promoter        complement(3355..3385)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    3393^3394
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR3           complement(3409..3533)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:kw-eztyb8e"">attR3</a>"
     primer          complement(3542..3557)
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     terminator      3601..3826
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     3856..3875
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /Sequence="TTGCATGCCCCCACTACAAT"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="16.7"
                     /modified_by="mamee"
                     /label="P38"
     promoter        3857..4253
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dgycryx"">pROLD</a>"
                     /label="pROLD"
     misc_feature    4237..4244
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    4369
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     CDS             4407..5204
                     /label="EgfpER"
     misc_feature    4434
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     4653..4672
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="599255191050670025ddd074-4560-4c74-accc
                     -96708a4156de"
                     /created_by="primer3"
                     /Sequence="CTCGTGACCACCCTGACCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P27"
     terminator      5233..5458
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="97.35%"
                     /Primary_Match="97.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     misc_feature    5325
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     T-DNA_border    5470..5700
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     5491..5510
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59925515908405006b6858d1-7919-4c68-b95c
                     -23f5accf37c0"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P14"
     misc_feature    5585
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5678..5707
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6319..6337
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="5992551019779700afd8ebeb-9ef7-401e-84f5
                     -15912cbcb494"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P1"
     regulatory      6886..7515
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     7111..7130
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="599255107675500056f5b87e-3bcf-4506-bce5
                     -736a92154f80"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P2"
     primer_bind     7908..7926
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="59925511345744007c1ff217-8860-4314-9dd2
                     -0048c4ea7d27"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P3"
     regulatory      7944..9017
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    7948
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     8712..8729
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="59925511919910003f0193c2-6ea4-43e2-b03b
                     -d95c65884b7b"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P4"
     rep_origin      9083..9277
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     9512..9531
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="599255124905490099ce6b29-8dda-4679-8be2
                     -8a3fa109f89d"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P5"
     regulatory      9651..9911
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(9947..10535)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     10336..10355
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="5992551305479000f6712abf-0f0f-4b5d-9ddc
                     -7b3de25a247a"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P6"
     misc_feature    10721^10722
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10733^10734
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10738..10741
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(10781..11572)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     11108..11127
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="59925513616404000982a264-a8b2-415e-aac6
                     -a92c2008c3de"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKCTAP"
                     /label="P7"
     Promoter        complement(11573..11847)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    11707^11708
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11718^11719
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11786..11787
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    11790^11791
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11823^11824
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgcgg tacccgggga tcctctagag ggcccgagcg gataacaatt tcacacagga
     1801 aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt tgaacgagaa
     1861 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     1921 taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat
     1981 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     2041 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat
     2101 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     2161 ggggacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
     2221 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     2281 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcggcaaaa gagccgttca
     2341 tttcaataaa ccgggcgacc tcagccatcc cttcttgatt ttccgctttc cagcgttcgg
     2401 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cgaaccggag atactgacat
     2461 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     2521 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg
     2581 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatcctcta
     2641 gattacgccc cgcctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2701 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2761 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2821 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2881 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2941 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     3001 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     3061 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     3121 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     3181 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     3241 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     3301 ttttagcttc cttagctcct gaaaatctcg acggatccta actcaaaatc cacacattat
     3361 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgcggccgcc atagtgactg
     3421 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     3481 attgatattt atatcatttt acgtttctcg ttcaacttta ttatacatag ttgataattc
     3541 actggccgtc gttttacaac gtcgtgactg ggaaaacgag ctcatctaac tgactagccg
     3601 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     3661 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     3721 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     3781 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgcag gaagcttgca
     3841 tgcctgcagg tcagcttgca tgcccccact acaatgaatt tgttcgtgaa ctattagttg
     3901 cgggccttgg catccgacta cctctgcggc aatattatat tccctgggcc caccgtgaac
     3961 ccaatttcgc ctatttattc attaccccca ttaacattga agtagtcatg atgggcctgc
     4021 agcacgttgg tgaggctggc acaactcatc catatacttt ctgaccggat cggcacatta
     4081 ttgtagaaaa cgcggaccca cagcgcactt tccaaagcgg tgccgcgtca gaatgcgctg
     4141 gcagaaaaaa attaatccaa aagtaccctc caagcagccc atataaacgc gtttacaaat
     4201 ccgctaacct caacaatttg agcagagaaa attcgcacct acaatagagg atccccgggt
     4261 accgagctcg aattctcaac acaacatata caaaacaaac gaatctcaag caatcaagca
     4321 ttctacttct attgcagcaa tttaaatcat ttcttttaaa gcaaaagcaa ttttctgaaa
     4381 attttcacca tttacgaacg atagccatgg tgaagactaa tctttttctc tttctcatct
     4441 tttcacttct cctatcatta tcctcggccg acatggtgag caagggcgag gagctgttca
     4501 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4561 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4621 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4681 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4741 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4801 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4861 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4921 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4981 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5041 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5101 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5161 tcactctcgg catggacgag ctgtacaaga aggacgagct gtaagcgccg cccggctgca
     5221 gcccggggga tccactagtt ctagagtccg caaaaatcac cagtctctct ctacaaatct
     5281 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     5341 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     5401 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     5461 aggcatgcaa gcttagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     5521 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     5581 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     5641 gccaaccaca gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaaa
     5701 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     5761 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     5821 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     5881 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     5941 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     6001 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     6061 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     6121 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     6181 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     6241 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     6301 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     6361 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     6421 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     6481 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     6541 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     6601 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     6661 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     6721 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     6781 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     6841 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     6901 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     6961 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     7021 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     7081 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     7141 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     7201 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     7261 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     7321 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     7381 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     7441 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     7501 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     7561 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     7621 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     7681 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     7741 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     7801 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     7861 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     7921 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     7981 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     8041 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     8101 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     8161 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     8221 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     8281 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     8341 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     8401 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     8461 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     8521 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     8581 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     8641 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     8701 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     8761 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     8821 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     8881 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     8941 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     9001 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     9061 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     9121 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     9181 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     9241 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     9301 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     9361 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     9421 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     9481 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     9541 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     9601 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     9661 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     9721 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     9781 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9841 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9901 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9961 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10021 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10081 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10141 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10201 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10261 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10321 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10381 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10441 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10501 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10561 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
    10621 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
    10681 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc
    10741 cgcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg
    10801 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
    10861 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
    10921 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
    10981 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
    11041 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
    11101 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
    11161 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
    11221 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
    11281 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
    11341 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
    11401 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
    11461 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
    11521 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta
    11581 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
    11641 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa
    11701 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
    11761 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg
    11821 aacaggctta tgtccactgg gttcgtgccc gaat
//
