LOCUS       pm42GW7,3               4929 bp    DNA     circular SYN 29-AUG-2022
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     attR4           1..124
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:l1-eztyg66"">attR4</a>"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
     promoter        149..179
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    233..892
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     UTR             915..1233
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     CDS             1234..1539
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(1580..1704)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
                     /Alternative_Match="96.83%; Adjusted-Interval-0:0/-1; <a
                     href=""urn:local:.:kr-ezty4ze"">attR2</a>"
     terminator      1762..1987
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S v2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:lk-f0ukkf4"">t35S v2</a>)"
                     /Alternative_Match="95.00%; Adjusted-Interval-0:-14/0;
                     t35S (<a href=""urn:local:.:2k-f1t986v"">t35S v1</a>)"
                     /label="t35S"
     rep_origin      2199..2654
                     /note="f1 replication origin derived from bacteriophage
                     f1"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F1"
                     /Transferred_Similarity="99.34%"
                     /Primary_Match="99.34%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8g-dk4mzop"">F1</a>"
                     /label="F1"
     promoter        2732..2836
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2837..3697
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.77%"
                     /Primary_Match="99.77%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3868..4456
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4820..4841
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
     primer          4841..4861
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="95.24%"
                     /Primary_Match="95.24%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
ORIGIN      
        1 caactttgta tagaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
       61 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      121 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      181 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      241 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      301 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      361 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      421 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      481 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      541 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      601 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      661 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      721 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      781 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
      841 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
      901 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
      961 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     1021 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     1081 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     1141 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     1201 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     1261 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     1321 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     1381 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     1441 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     1501 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     1561 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     1621 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     1681 ttcagctttc ttgtacaaag tggtgatatc ccgcggccat ggcggccggg agcatgcgac
     1741 gtcgatctaa ctgactagcc gcggccatgc tagagtccgc aaaaatcacc agtctctctc
     1801 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     1861 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     1921 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     1981 gtgacctgca ggcatgcgac gtcgggccca attcgcccta tagtgagtcg tattacaatt
     2041 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2101 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2161 gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt
     2221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     2281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2401 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2521 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     2581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2641 aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     2701 cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     2761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     2821 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     2881 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     2941 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3001 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3061 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3121 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3181 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3241 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3301 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3361 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3421 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3481 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     3541 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3601 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3661 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3721 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3781 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3841 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3901 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3961 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4021 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4081 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4141 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4201 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4261 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4321 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4381 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4441 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4501 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4561 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4621 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     4681 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     4741 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     4801 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     4861 tgattacgcc aagctattta ggtgacacta tagaatactc aagctatgca tccaacgcgt
     4921 tgggagctc
//
