LOCUS       pKm42GW,3               9887 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...ll-doz2me2
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|457775207|                                                 
            VNTDBDATE|457775207|                                               
            LSOWNER|                                                           
            VNTNAME|pKm42GW,3 rc|                                              
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
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                     #245=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 14352856
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                                   #246=(CGroupWidget                          
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            #249=(CGroupWidget                                                 
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            #252=(CGroupWidget                                                 
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            #255=(CGroupWidget                                                 
                  (CWidget 1 (10 #7# 0) 1 2 0 0 Nil 40165588 100)              
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            #261=(CGroupWidget                                                 
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            #270=(CGroupWidget                                                 
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            #273=(CGroupWidget                                                 
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            #279=(CGroupWidget                                                 
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FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="5986608989109000278badd0-16a6-44d5-b06d
                     -a2fd5d27458d"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1727)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kanamycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Kana (<a
                     href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="Kana"
     CDS             complement(648..1442)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     NptII (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="NptII"
     primer_bind     771..790
                     /%GC="50.0"
                     /Hairpin_Tm="46.1"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="59866093353179004dbabce8-3720-4eed-a122
                     -cda48b5b5ed1"
                     /created_by="primer3"
                     /Sequence="AGCCAACGCTATGTCCTGAT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P24"
     primer_bind     1384..1403
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="5986609368750400ef99b017-624e-479a-89bf
                     -c92640ec9c2e"
                     /created_by="primer3"
                     /Sequence="AATAGCCTCTCCACCCAAGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P25"
     promoter        complement(1455..1727)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1630
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1728..1732
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1736..1738
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     attR4           1753..1876
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; attR4'
                     (<a href=""urn:local:.:l1-eztyg66"">attR4</a>)"
                     /Alternative_Match="99.19%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:o4-f0pbp83"">attR4</a>"
                     /Color="#00cccc"
                     /modified_by="mamee"
     promoter        1901..1931
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    1955
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1985..2644
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     2140..2159
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="5986609047787700792998a4-cb65-4e73-b215
                     -d9f18d9aa15f"
                     /created_by="primer3"
                     /Sequence="GCACAAGTTTTATCCGGCCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P12"
     misc_feature    2569
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     UTR             2667..2985
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2759
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2923..2942
                     /%GC="55.0"
                     /Hairpin_Tm="35.1"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5986609106041300a2b42957-25bc-4320-bb68
                     -696bf0db609f"
                     /created_by="primer3"
                     /Sequence="CTGAGGTCGCCCGGTTTATT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P13"
     misc_feature    2977
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             2986..3291
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(3332..3456)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     T-DNA_border    3503..3733
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /Alternative_Match="99.50%; Adjusted-Interval-0:0/-30; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     primer_bind     3524..3543
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5986609164862400b72709be-fc6a-4906-ba73
                     -4ceaaa79e70d"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P14"
     misc_feature    3618
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3701..3730
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     4352..4370
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="59866085850903007b73bc4b-d120-4548-a8d5
                     -54b1f712278b"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P1"
     regulatory      4919..5548
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     5144..5163
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="598660864564450038d7ac53-3e83-4990-9da8
                     -069754cf98f4"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P2"
     primer_bind     5941..5959
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="59866087025539005e2a60a2-b4e7-4cbf-b04f
                     -b4393249a77c"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P3"
     regulatory      5977..7050
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    5981
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6745..6762
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="5986608758710900c699f648-9035-4953-8c43
                     -f0fed97a14ca"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P4"
     rep_origin      7116..7310
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     7545..7564
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5986608817696300cfb77f42-6a58-468a-8fe2
                     -f18a6344e306"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P5"
     regulatory      7684..7944
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(7980..8568)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     8369..8388
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="59866088747191001516d70e-e60d-4dbc-964e
                     -608fdf4fdd31"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P6"
     misc_feature    8754^8755
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    8766^8767
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    8771..8774
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(8814..9605)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     9141..9160
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5986608931655600e84f40bb-1d35-409a-89a6
                     -6a3089f91bd5"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pKm42GW,3"
                     /label="P7"
     Promoter        complement(9606..9880)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    9740^9741
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9751^9752
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9819..9820
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    9823^9824
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9856^9857
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gctagagtcc gaaccccaga gtcccgctca gaagaactcg
      661 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
      721 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
      781 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
      841 ccattttcca ccatgatatt cggcaagcag gcatcgccgt gggtcacgac gagatcctcg
      901 ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
      961 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     1021 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     1081 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     1141 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     1201 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     1261 tggagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     1321 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     1381 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     1441 atgcctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     1501 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     1561 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     1621 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     1681 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
     1741 taattcgagc tccaactttg tatagaaaag ttgaacgaga aacgtaaaat gatataaata
     1801 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     1861 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     1921 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     1981 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2041 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2101 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2161 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     2221 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2281 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2341 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2401 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2461 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2521 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     2581 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2641 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     2701 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     2761 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     2821 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     2881 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     2941 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     3001 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3061 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3121 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3181 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     3241 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     3301 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     3361 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3421 ttacgtttct cgttcagctt tcttgtacaa agtggtgata tcccgcggcc atggcggccg
     3481 ggagcatgcg acgtcgggcc caagcttagc ttgagcttgg atcagattgt cgtttcccgc
     3541 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     3601 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca
     3661 tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt aaagtacttt
     3721 aaagtacttt aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     3781 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     3841 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     3901 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     3961 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     4021 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     4081 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     4141 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     4201 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     4261 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     4321 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     4381 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     4441 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     4501 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     4561 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     4621 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     4681 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     4741 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     4801 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     4861 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     4921 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     4981 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     5041 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     5101 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     5161 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     5221 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     5281 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     5341 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     5401 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     5461 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     5521 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     5581 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     5641 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     5701 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     5761 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     5821 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     5881 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
     5941 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     6001 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     6061 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     6121 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     6181 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     6241 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     6301 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     6361 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     6421 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     6481 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     6541 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     6601 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     6661 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     6721 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     6781 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     6841 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     6901 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     6961 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     7021 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     7081 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     7141 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     7201 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     7261 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     7321 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     7381 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     7441 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     7501 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     7561 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     7621 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     7681 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7741 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     7801 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7861 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8401 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8641 atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     8701 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg
     8761 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg
     8821 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
     8881 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
     8941 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
     9001 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
     9061 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
     9121 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
     9181 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
     9241 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
     9301 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
     9361 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
     9421 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
     9481 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
     9541 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
     9601 cccatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     9661 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     9721 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     9781 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     9841 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg cccgaat
//
