LOCUS       pGGIB-U5-pPvUBI2-ZmGRF1-GIF1-tNOS-U6        6593 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.e4-h75v1ra
VERSION     urn.local.psbuser.e4-h75v1ra
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C875                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG1111                                    
            ##E.coli_Glycerol_Stock-END##                                      
            ##Collection_Info_PSB-START##                                      
            Bacterial Selection :: Kanamycin                                   
            ##Collection_Info_PSB-END##
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U5"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dgycryx"">U5</a>"
                     /label="U5"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        71..1931
                     /modified_by="thjac"
                     /label="PvUbi2"
     5'UTR           763..859
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="PvUbi2 5'UTR"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:3q-cb8uu5h"">PvUbi2
                     5'UTR</a>"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PvUBI2 promoter"
                     /Transferred_Similarity="98.97%"
                     /Primary_Match="98.97%; Adjusted-Interval-0:0/0; PvUbi2
                     5'UTR (<a
                     href=""urn:local:laurenspauwels:51-cb8w46g"">PvUBI2
                     promoter</a>)"
                     /Alternative_Match="98.97%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:3q-cb8uu5h"">PvUbi2
                     5'UTR</a>"
                     /label="PvUbi2 5'UTR"
     intron          860..1931
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PvUbi2 intron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; PvUbi2
                     intron1 (<a
                     href=""urn:local:laurenspauwels:3r-cb8uu5h"">PvUbi2
                     intron</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     PvUbi2 intron1 (<a
                     href=""urn:local:laurenspauwels:51-cb8w46g"">PvUBI2
                     promoter</a>)"
                     /label="PvUbi2 intron1"
     Linker          1936..1959
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:24f-gh5671n"">Linker</a>"
     CDS             1966..3918
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmGRF1-linker-ZmGIF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ZmGRF-ZmGIF (<a
                     href=""urn:local:lapau:1y-dttkko1"">ZmGRF1-linker-ZmGIF1</
                     a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/0;
                     ZmGRF-ZmGIF (<a
                     href=""urn:local:lapau:9u-dc98np8"">ZmGRF-linker-ZmGIF</a>
                     )"
                     /label="ZmGRF-ZmGIF"
     terminator      3923..4190
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2 Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:fi-eaul7he"">2 Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fj-eaul7he"">3 Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     misc_feature    4201..4240
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U6"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7u-dgycryx"">U6</a>"
                     /label="U6"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(4241..4258)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(4286..4306)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4367..4417
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4418..5227
                     /modified_by="wacae"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5374..5962
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6562..6582
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctacc aggatacata gattaccaca actccgagcc cttccaccct
       61 cgagatacct gaagccaact aaacaagacc ataaccatgg tgacatttga catagttgtt
      121 tactacttgc ttgagcccca cccttgctta tcggttgaac attacaagat acactgcggg
      181 tggcctaagg cacaccgtcc gaaaccggca aaccaagcct gatcgccgaa atccaaaatc
      241 actaccggca atctctaaag tttatttcat ccttatatga cgaggaaaga aaagaagaga
      301 gaaataatat cttaacttct aaatcagtcg cgtcaacttt ctcggctaag aaagtgagca
      361 ctatcatttc ggacaccatg tcatgagtgc cgacttgcca tatcttatta tattcttatt
      421 tatttaatta taatcccatt gcaatacgtc tattctatca tggcctgcca ctaacgctcc
      481 gtctaacgtc gttaagccat tgtcataagc ggctgctcaa aactcttccc ggtggaggcg
      541 aggcgttaac ggcgtctaca aatctaacgg ccaccaacca tccagccgcc tctcgaaagc
      601 tccgctccga tcgcggaaat tgcgtggcgg agacgagcgg gctcctctca cacggcccgg
      661 aaccgtcacg gcacgggtgg gggattcctt ccccaaccct ccccacctct cctccccccg
      721 tcgcagccca taaatacaag gccctccgcg cctcttccca caatctcaca tcgtctcatc
      781 gttcggagcg cacaaccccc gggttccaaa tccaaattgc tcttctcgcg accctcggcg
      841 atccttcccc cgcttcaagg tacggcgatc gtctcccccg tcctcttgcc ccatctcctc
      901 gctcggcgtg gtttggtggt tctgcttggt ctgtggctag gaactaggct gaggcgttga
      961 cgaaatcatg ctagatccgc gtgtttcctg atcgtgggtg gctgggaggt ggggttttcg
     1021 tgtagatctg atcggttccg ctgtttatcc tgtcatgctc atgtgatttg tggggatttt
     1081 aggtcgtttg tccgggaatc gtggggttgc ttctaggctg ttcgtagatg agatcgttct
     1141 cacgatctgc tgggtcgctg cctaggttca gctaggtctg ccctgttttt gggttcgttt
     1201 tcgggatctg tacgtgcatc tattatctgg ttcgatggtg ctagctagga acaaacaact
     1261 gattcgtccg atcgattgtt ttgttgccat gtgcaaggtt aggtcgttat ctgattgctg
     1321 tagatcagag tagaataaga tcatcacaag ctagctcttg ggcttattat gaatctgcgt
     1381 ttgttgcatg attaagatga ttatgctttt tcttatgctg ccgtttgtat atgatgcggt
     1441 agcttttaac tgaatagcac acctttcctg tttagttaga ttagattaga ttgcatgata
     1501 gatgaggata tatgctgcta catcagtttg atgattctct ggtacctcat aatcaactag
     1561 ctcatgtgct taaattgaaa ctgcatgtgc cacatgatta agatgctaag attggtgaag
     1621 atatatacgc tgctgttcct ataggatcct gtagctttta cctggtcaac atgcatcgtc
     1681 ctgttatgga tagatatgca tgatagatga agatatgtac tgctacaatt tgatgattct
     1741 tttgtgcacc tgatgatcat gcatgctctt tgcccttact ttgatatact tggatgatgg
     1801 catgcttagt actaatgatg tgatgaacac acatgacctg ttggtatgaa tatgatgttg
     1861 ctgtttgctt gtgatgagtt ctgtttgttt actgctaggc acttaccctg ttgtctggtt
     1921 ctcttttgca gaacagtatt cagtcgactg gtaccaacag gctccatggc gatgccgtat
     1981 gcctctcttt ccccggcagg cgccgccgac caccgctcct ccacagccac ggcgtccctc
     2041 gtccccttct gccgctccac tccgctctcc gcgggcggcg ggctgggcga ggaggacgcc
     2101 caggcgagcg cgaggtggcc ggccgcgagg ccggtggtgc cgttcacgcc ggcgcagtac
     2161 caggagctgg agcagcaggc gctcatatac aagtacctgg tggcgggcgt gcccgttccg
     2221 ccggatctcg tggttccaat ccgccgcggc ctcgactccc tcgcaacccg cttctacggc
     2281 caacccacac tcgggtacgg accgtacctg gggaggaaac tggatccgga gcccggccgg
     2341 tgccggcgaa cggacggcaa gaagtggcgg tgctccaagg aggccgcccc ggactccaag
     2401 tactgcgagc gccacatgca ccgcggccgc aaccgttcaa gaaagcctgt ggaaacgcag
     2461 ctcgcgcccc agtcccaacc gcccgccgcc gcagccgtct ccgccgctcc gcccctggca
     2521 gccgccgccg ccgccaccac caacggcagc ggcttccaga accactctct ctacccggcc
     2581 atcgccggca gcactggtgg tggaggagga gttggcgggt ccggcaatat ctcctccccg
     2641 ttctcctcgt cgatgggggg atcgtctcag ctgcacatgg acagtgctgc cagctactcc
     2701 tacgcagctc ttggtggtgg aactgcaaag gatctcaggt acaacgctta cggaataaga
     2761 tctctggcgg acgagcacaa ccagctgatc gcagaagcca tcgactcgtc gatagagagc
     2821 cagtggcgcc tccccagctc gtcgttcccg ctctcgagct acccacatct cggggcgctg
     2881 ggcgacctgg gcggccagaa cagcacggtg agctcgctgc cgaagatgga gaagcagcag
     2941 ccgccctcgt ccttcctagg gaacgacacc ggggccggca tggccatggg ctccgcctcc
     3001 gcgaagcagg agggccagac gctgcggcac ttcttcgacg agtggcccaa ggcgcgggac
     3061 tcctggccgg gcctctccga cgagacggcc agcctcgcct cgttcccccc ggcgacccag
     3121 ctgtcgatgt ccatacccat ggcgtcctcc gacttctccg tggccagctc ccagtcgccc
     3181 aacggtgagt cgcgtacgtt cctgctggcc acggaccgaa gggcggccgc tgccatgcag
     3241 cagcaacacc tgatgcagat gaaccagaac atgatggggg gctacacctc tcctgccgcc
     3301 gtgaccaccg atctcatcca gcagtacctg gacgagaaca agcagctgat cctggccatc
     3361 ctcgacaacc agaacaatgg caaggcggag gagtgcgaac ggcaccaagc taagctccag
     3421 cacaacctca tgtacctggc cgccatcgct gacagccagc cgccacagac cgcgccacta
     3481 tcacagtacc cgtccaacct gatgatgcag ccgggccctc ggtacatgcc accgcagtcc
     3541 gggcagatga tgaacccgca gtcgctgatg gcggcgcggt cctccatgat gtacgcgcac
     3601 ccgtccctgt cgccactcca gcagcagcag gcggcgcacg gacagctggg tatggctcca
     3661 gggggcggcg gtggcggcac gaccagcggg ttcagcatcc tccacggcga ggccagcatg
     3721 ggcggtggtg gtgctggcgc aggcgccggc aacaacatga tgaacgccgg catgttctcg
     3781 ggctttggcc gcagcggcag tggcgccaag gaagggtcga cctctctgtc ggttgacgtc
     3841 cggggtggaa ccagctccgg cgcgcagagc ggggacggcg agtacctcaa agtcggcacc
     3901 gaggaagaag gcagttagtc agctagagtc aagcagatcg ttcaaacatt tggcaataaa
     3961 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     4021 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     4081 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     4141 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcga gtattctaga
     4201 ctcgttcgct gccacctaag aatactctac ggtcacatac tagggataac agggtaatga
     4261 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     4321 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     4381 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     4441 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     4501 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     4561 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     4621 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     4681 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     4741 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     4801 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     4861 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     4921 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     4981 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     5041 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     5101 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     5161 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     5221 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     5281 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     5341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6061 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6241 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     6301 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     6361 cgccacccct tcgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     6421 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt catccgactg
     6481 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     6541 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccc
//
