LOCUS       pGGIB-U3-pBdEF1α-Linker-YFP-Linker-NLS-BdUbiT-U4        5932 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  tBdUbi: 2bp + triple stopcodon from G7T.
ACCESSION   urn.local.psbuser.8p-h75u0yj
VERSION     urn.local.psbuser.8p-h75u0yj
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C731                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG930                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7r-dgycryx"">U3</a>"
                     /label="U3"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        71..2070
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBdEF1a.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:26-c531q5y"">pBdEF1a.2</a>"
                     /label="pBdEF1a.2"
     Linker          2075..2098
                     /created_by="psbuser"
                     /modified_by="psbuser"
                     /label="B-dummy gene"
     CDS             2105..2821
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GFP(Q70stop)"
                     /Transferred_Similarity="98.61%"
                     /Primary_Match="98.61%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>"
                     /Alternative_Match="98.61%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:hp-ecphyqg"">GFP(Q81stop)</a>"
                     /Alternative_Match="98.47%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:33-c4nnd56"">GFP</a>"
                     /Alternative_Match="98.33%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:hw-ecpi0my"">GFP(R110stop)</a>"
                     /Alternative_Match="97.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:hg-ecphww2"">GFP FLARE(T63S)</a>"
                     /Alternative_Match="97.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:i2-ecpi3uh"">GFP(Q70stop-R74K)</a>"
                     /modified_by="psbuser"
                     /label="YFP"
     Linker          2825..2872
                     /created_by="wacae"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:.:6j-dgycryx"">(GS)8</a>)"
     NLS             2897..3121
                     /created_by="wacae"
                     /X-Transferred_From="NLS GreenGate"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9d-dgycryy"">NLS N7</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:-24/0; N7
                     (<a href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     misc            3131..3143
                     /created_by="psbuser"
                     /modified_by="psbuser"
                     /label="triple stopcodon"
     terminator      3144..3476
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tBdUbi1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BdUbi1T
                     (<a href=""urn:local:lapau:1x-e60q7hc"">tBdUbi1</a>)"
                     /label="BdUbi1T"
     Linker          3481..3529
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     misc_feature    3540..3579
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7s-dgycryx"">U4</a>"
                     /label="U4"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(3580..3597)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(3625..3645)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3706..3756
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3757..4566
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4713..5301
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5901..5921
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctagc actgaaggtc ctcaatcgca ctggaaacat caaggtcgct
       61 cgagatacct agatttgtga ttgttcagct acatgctcct tgaagcacct ttattctact
      121 ataaattagt tcgttggacg agactattag accacatgct agccatgctt gttcgtagtt
      181 gtcttcaccg ccattgcaaa aattgtcaat aaatatttag agtgggtggc atcagaaaaa
      241 catctctagt ggactctctt cctatcatag ctactcgggc tgtagataga acgagggcac
      301 aagagttggg tggcgtaggt ttactcgtga cctcaactct tttggctgtg tcttacgtct
      361 aagatgggtt tggcatgtga gaaacatagg tctaagcaat tcatgttagg gctgttgcat
      421 tgttgttgca tcaaccaaat gtccagatag cagttcatgc tacatctagt tgaaaaccct
      481 catcattagg cggaacatgt gttctttttt agcatagtca aagtcagatt gcggcactcg
      541 ctcatccacg gaaagaattt tccctgtgca ggcatctcga tcaaaagacg caaattaatt
      601 tttgaatagc gatataacaa tatctaatta acgtttcttg ttttctgcga aatgtctttc
      661 atcataaaat gagtcatctc gatgagccca agtgacatag cccaacaccc caccccacca
      721 ataaaagtga agaaaacatg ttgggaaaac tataccaagt aaaatacgag ttgttctaaa
      781 gaaaaagtaa agtacgagtt agatcgcacc ctgtcctgga gtgtggcttg atgatccaac
      841 tcctagcatt gtatccctgt ttttggatga tgtaactatt atttacaatg aataaagagg
      901 tgttttacta gtaaaaaaat cttgagggga ggagaaaata atggaggtct tttttcaaac
      961 cgatggacta ttatttttag tgaaagagaa taatattatt ggaaaaatta ttctatccac
     1021 ttattttata ttggcagaat acaaagaatg gtggggtcca cgcggaactt gcggcccccg
     1081 aaacctatcg agggcgcggt acccaagcaa ggaacggagg aaacttgcgg ggcccgaaac
     1141 ctagtgataa aaggcatatc atccacacga tgaagatctg acggaccata tctcccacca
     1201 cggaaagcca tcagacgagg atcagacggc caggaaggaa ccctagcgcc cgccggtgcc
     1261 aatataaagc gccactctct ctcgtcttaa gccccagcct ctccattccc ctctccctct
     1321 cgccgccgcc gtctccttct cctactccct tcgaggtgtg ttgttcatcc gtcccgaatc
     1381 catccatccc ctcttcagat gtgttgttca tggctctaat agctctagat ctgcttgttt
     1441 gtgttgttta gctctagatc tactcgcgcg cgcttctctc tcgatctcct gtagaacaat
     1501 tttggttggt tttttgtgca tatccatggt aattttgtct gcaatatgga ggaggctttc
     1561 taagctccta cgtagcatcg atctttagaa ttccctcggt ttctgtttat ttcttcgcga
     1621 gggctctctg ttatctgtag gagtagctgt aagcgcggtt cgttacggat taatcgtcat
     1681 gcttagttga acctatcggt cgaaggattt gtgtgggttg tcgtgtagaa ttgacaccat
     1741 ctacttactg tactgatatg ccgatctgta ggatactctt cattactttt gtttactgct
     1801 agttgtggtg tagatttagc attctcaaac ccatgctgta gcgtttctaa tattgttaca
     1861 tagatctacc ggtgcctgtt aattgtattc gatcgggcgt ttctacatct gtccgcccac
     1921 ctagttttat atgtggtaat caaaattgcg ttgacttcgt gatgctgtct gtgtactgtt
     1981 tttaatcgct cttacttaga tgatcaacat ggtgatggtt acgatttact gttttctaat
     2041 ccctgttact tcgatgctgc agtttcagcc aacagtattc agtcgactgg taccaacagg
     2101 ctccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2161 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2221 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2281 caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     2341 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2401 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2461 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2521 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2581 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2641 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2701 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2761 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2821 gtcaggttct ggttcaggtt caggttctgg atctggaagt ggatctggat cagcggccgc
     2881 atctgaattc aagcgtgaag agcaagcaag gaaagctaag gtgaacaatg agaaaaagac
     2941 ggaaatagtg aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc tgaaaagaaa
     3001 ggactctgag gatggaaacg agggtgagga agaagaagct tcttcgaaac cgaaaaagcc
     3061 aaaagttgct ctttctcatc ttcaggacat tgacgacaca gaagctgatc aagaagaaga
     3121 gtaactgctc tgagctaagc taggcttctg ccgaactggt tcacagtctg ctgcccttgg
     3181 tggtctgccc cttagtggtc atgccttttg ttatgtgtct tgcgtcccaa tcctgtatcg
     3241 tttgtgtgaa catctctgct gctgtatagc agcttgaatc ctgttatgaa tttgtgaacc
     3301 tgaaccttgt tccgtgaatc atgttatgaa taagtgaacc tgaaccttgt tccgtgatta
     3361 ttgttacaat ctgttgtgcc gtatggttgg tcgtgtgtga tttatgttga actggagaac
     3421 caagttcgtt ccaggacata ttgcaaccta agctaaacca tgtagaacta cttgttacta
     3481 taagctaggt agtaactagt gcgactgtct agaatcgcgt atgcgagtgg tattctagac
     3541 tgacctcctg ccagcaatag taagacaaca cgcaaagtct agggataaca gggtaatgat
     3601 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     3661 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     3721 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     3781 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     3841 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     3901 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     3961 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     4021 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     4081 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     4141 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     4201 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     4261 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     4321 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     4381 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     4441 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     4501 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     4561 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     4621 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     4681 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4741 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4801 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4861 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4921 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4981 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5041 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5101 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5161 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5221 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5281 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5341 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5401 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5461 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5521 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5581 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     5641 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     5701 gccacccctt cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     5761 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc atccgactga
     5821 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     5881 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
