LOCUS       pGGIB-U3-pBdEF1α.2-tdTomato-tNOS-U4        6207 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.wd-hau96ru
VERSION     urn.local.psbuser.wd-hau96ru
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C351                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG354                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7r-dgycryx"">U3</a>"
                     /label="U3"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        71..2070
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBdEF1a.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:26-c531q5y"">pBdEF1a.2</a>"
                     /label="pBdEF1a.2"
     Linker          2075..2098
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:24f-gh5671n"">Linker</a>"
     CDS             2105..3532
                     /label="tdTomato"
     terminator      3537..3804
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2 Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:fi-eaul7he"">2 Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fj-eaul7he"">3 Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     misc_feature    3815..3854
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7s-dgycryx"">U4</a>"
                     /label="U4"
                     /note="Geneious type: Golden Gibson Cloning site"
     Junction        3853
                     /standard_name="junction 1"
     misc_difference complement(3855..3872)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(3900..3920)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3981..4031
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4032..4841
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4988..5576
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6176..6196
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctagc actgaaggtc ctcaatcgca ctggaaacat caaggtcgct
       61 cgagatacct agatttgtga ttgttcagct acatgctcct tgaagcacct ttattctact
      121 ataaattagt tcgttggacg agactattag accacatgct agccatgctt gttcgtagtt
      181 gtcttcaccg ccattgcaaa aattgtcaat aaatatttag agtgggtggc atcagaaaaa
      241 catctctagt ggactctctt cctatcatag ctactcgggc tgtagataga acgagggcac
      301 aagagttggg tggcgtaggt ttactcgtga cctcaactct tttggctgtg tcttacgtct
      361 aagatgggtt tggcatgtga gaaacatagg tctaagcaat tcatgttagg gctgttgcat
      421 tgttgttgca tcaaccaaat gtccagatag cagttcatgc tacatctagt tgaaaaccct
      481 catcattagg cggaacatgt gttctttttt agcatagtca aagtcagatt gcggcactcg
      541 ctcatccacg gaaagaattt tccctgtgca ggcatctcga tcaaaagacg caaattaatt
      601 tttgaatagc gatataacaa tatctaatta acgtttcttg ttttctgcga aatgtctttc
      661 atcataaaat gagtcatctc gatgagccca agtgacatag cccaacaccc caccccacca
      721 ataaaagtga agaaaacatg ttgggaaaac tataccaagt aaaatacgag ttgttctaaa
      781 gaaaaagtaa agtacgagtt agatcgcacc ctgtcctgga gtgtggcttg atgatccaac
      841 tcctagcatt gtatccctgt ttttggatga tgtaactatt atttacaatg aataaagagg
      901 tgttttacta gtaaaaaaat cttgagggga ggagaaaata atggaggtct tttttcaaac
      961 cgatggacta ttatttttag tgaaagagaa taatattatt ggaaaaatta ttctatccac
     1021 ttattttata ttggcagaat acaaagaatg gtggggtcca cgcggaactt gcggcccccg
     1081 aaacctatcg agggcgcggt acccaagcaa ggaacggagg aaacttgcgg ggcccgaaac
     1141 ctagtgataa aaggcatatc atccacacga tgaagatctg acggaccata tctcccacca
     1201 cggaaagcca tcagacgagg atcagacggc caggaaggaa ccctagcgcc cgccggtgcc
     1261 aatataaagc gccactctct ctcgtcttaa gccccagcct ctccattccc ctctccctct
     1321 cgccgccgcc gtctccttct cctactccct tcgaggtgtg ttgttcatcc gtcccgaatc
     1381 catccatccc ctcttcagat gtgttgttca tggctctaat agctctagat ctgcttgttt
     1441 gtgttgttta gctctagatc tactcgcgcg cgcttctctc tcgatctcct gtagaacaat
     1501 tttggttggt tttttgtgca tatccatggt aattttgtct gcaatatgga ggaggctttc
     1561 taagctccta cgtagcatcg atctttagaa ttccctcggt ttctgtttat ttcttcgcga
     1621 gggctctctg ttatctgtag gagtagctgt aagcgcggtt cgttacggat taatcgtcat
     1681 gcttagttga acctatcggt cgaaggattt gtgtgggttg tcgtgtagaa ttgacaccat
     1741 ctacttactg tactgatatg ccgatctgta ggatactctt cattactttt gtttactgct
     1801 agttgtggtg tagatttagc attctcaaac ccatgctgta gcgtttctaa tattgttaca
     1861 tagatctacc ggtgcctgtt aattgtattc gatcgggcgt ttctacatct gtccgcccac
     1921 ctagttttat atgtggtaat caaaattgcg ttgacttcgt gatgctgtct gtgtactgtt
     1981 tttaatcgct cttacttaga tgatcaacat ggtgatggtt acgatttact gttttctaat
     2041 ccctgttact tcgatgctgc agtttcagcc aacagtattc agtcgactgg taccaacagg
     2101 ctcaatggtg agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat
     2161 ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2221 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     2281 ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga
     2341 catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2401 cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct
     2461 gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa
     2521 gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa
     2581 gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa
     2641 gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa
     2701 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
     2761 gggccgccac cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg
     2821 caccgcctcc tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt
     2881 gcgcatggag ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2941 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt
     3001 cgcctgggac atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc
     3061 cgccgacatc cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     3121 gatgaacttc gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg
     3181 cacgctgatc tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat
     3241 gcagaagaag accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt
     3301 gctgaagggc gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga
     3361 gttcaagacc atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga
     3421 caccaagctg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg
     3481 ctccgagggc cgccaccacc tgttcctgta cggcatggac gagctgtaca agtcagctag
     3541 agtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     3601 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     3661 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     3721 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     3781 gtgtcatcta tgttactaga tcgagtattc tagactgacc tcctgccagc aatagtaaga
     3841 caacacgcaa agtctaggga taacagggta atgatatccc ctatagtgag tcgtattaca
     3901 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3961 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     4021 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     4081 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     4141 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     4201 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     4261 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     4321 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     4381 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     4441 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     4501 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     4561 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     4621 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     4681 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     4741 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     4801 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     4861 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     4921 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5101 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5761 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5821 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     5881 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     5941 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cccttcgggc cgttgcttca
     6001 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     6061 aacagataaa acgaaaggcc cagtcatccg actgagcctt tcgttttatt tgatgcctgg
     6121 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     6181 acgacggcca gtcttaagct cgggccc
//
