LOCUS       pGGIB-U1-pZmUBI.2-zCas9-tNOS-U2        9009 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.mb-hau6k64
VERSION     urn.local.psbuser.mb-hau6k64
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C319                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG311                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7p-dgycryx"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        71..2058
                     /vntifkey=29
                     /modified_by="laurenspauwels"
                     /modified_by="mamee"
                     /label="pZmUBI"
     intron          1049..2058
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     tag             2066..2131
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="3xFLAG"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a6-dgycryz"">3xFLAG</a>"
                     /label="3xFLAG"
     NLS             2138..2158
                     /created_by="wacae"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NLS SV40 - short"
                     /Transferred_Similarity="95.24%"
                     /Primary_Match="95.24%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9e-dgycryz"">NLS SV40 - short</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; SV40
                     (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     CDS             2183..6283
                     /Transferred_From="ZmCas9"
                     /Transferred_Similarity="100.00%"
                     /label="zCAS9"
     NLS             6284..6331
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS Nucleoplasmin"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Nucleoplasmin (<a href=""urn:local:.:9z-dgycryz"">NLS
                     Nucleoplasmin</a>)"
                     /label="Nucleoplasmin"
     terminator      6340..6607
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2 Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:fi-eaul7he"">2 Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fj-eaul7he"">3 Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     misc_feature    6618..6657
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7q-dgycryx"">U2</a>"
                     /label="U2"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(6658..6675)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(6703..6723)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        6784..6834
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    6835..7644
                     /modified_by="wacae"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      7791..8379
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          8978..8998
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctaca ttactcgcat ccattctcag gctgtctcgt ctcgtctcct
       61 cgagatacct gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat
      121 gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt
      181 atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct atagtactac
      241 aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca
      301 attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc
      361 tttttttttg caaatagctt cacctatata atacttcatc cattttatta gtacatccat
      421 ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct attttattct
      481 attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt
      541 agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt
      601 aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc
      661 gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
      721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
      781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
      841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
      901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1261 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     1321 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     1381 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     1441 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat
     1501 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     1561 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     1621 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     1681 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     1741 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     1801 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     1861 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     1921 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     1981 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     2041 ttggtgttac ttctgcaggg ctatggatta caaggaccac gacggggatt acaaggacca
     2101 cgacattgat tacaaggatg atgatgacaa gatggctccg aagaagaaga ggaaggttgg
     2161 catccacggg gtgccagctg ctgacaagaa gtactcgatc ggcctcgata ttgggactaa
     2221 ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga agttcaaggt
     2281 cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc tcctgttcga
     2341 cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc ggtacacgcg
     2401 caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg cgaaggttga
     2461 cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata agaagcacga
     2521 gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga agtaccctac
     2581 gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc tccgcctgat
     2641 ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg agggggatct
     2701 caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga cgtacaacca
     2761 gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta tcctgtccgc
     2821 taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg gcgagaagaa
     2881 gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca acttcaagtc
     2941 gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat acgacgatga
     3001 cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc tcgctgccaa
     3061 gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg agattacgaa
     3121 ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct
     3181 cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct tcttcgatca
     3241 gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg agttctacaa
     3301 gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg tgaagctcaa
     3361 tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc cacaccagat
     3421 tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc ctttcctcaa
     3481 ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact acgtcggccc
     3541 actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg agacgatcac
     3601 gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt tcatcgagag
     3661 gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc actcgctcct
     3721 gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga ccgagggcat
     3781 gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc tcctgttcaa
     3841 gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga agattgagtg
     3901 cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc tggggaccta
     3961 ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg agaatgagga
     4021 tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga tgatcgagga
     4081 gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc tcaagaggag
     4141 gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc gggacaagca
     4201 gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc gcaatttcat
     4261 gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg ctcaggtgag
     4321 cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc cagctatcaa
     4381 gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca tgggcaggca
     4441 caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc agaagggcca
     4501 gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc tggggtccca
     4561 gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc tctacctgta
     4621 ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta acaggctcag
     4681 cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact ccattgacaa
     4741 caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc cttcagagga
     4801 ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc tgatcacgca
     4861 gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc tggacaaggc
     4921 gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg ttgcgcagat
     4981 tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc gcgaggtgaa
     5041 ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc agttctacaa
     5101 ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg cggtggtcgg
     5161 cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg gggactacaa
     5221 ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca aggctaccgc
     5281 caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca ctctggccaa
     5341 tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg agatcgtgtg
     5401 ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac aagtgaatat
     5461 cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc tgcctaagcg
     5521 gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt acggcgggtt
     5581 cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc
     5641 caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc gctccagctt
     5701 cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga agaaggacct
     5761 gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga agcggatgct
     5821 ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca agtatgtgaa
     5881 cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg acaacgagca
     5941 gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg agcagatttc
     6001 cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc tctccgcgta
     6061 caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc atctcttcac
     6121 cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta tcgatcgcaa
     6181 gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt cgattaccgg
     6241 cctctacgag acgcgcatcg acctgtctca gctcgggggc gacaagcggc cagcggcgac
     6301 gaagaaggcg gggcaggcga agaagaagaa gtgattcagc tagagtcaag cagatcgttc
     6361 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     6421 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     6481 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     6541 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     6601 agatcgagta ttctagagct gggagttcgt agacggaaac aaacgcagaa tccaagctag
     6661 ggataacagg gtaatgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     6721 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     6781 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     6841 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     6901 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     6961 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     7021 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     7081 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     7141 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     7201 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     7261 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     7321 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     7381 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     7441 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     7501 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     7561 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     7621 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     7681 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     7741 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7801 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7921 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8401 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8461 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8521 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8581 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8641 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     8701 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     8761 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     8821 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     8881 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     8941 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     9001 ctcgggccc
//
