LOCUS       pGG-E-tBdUBI-F          3034 bp    DNA     circular     05-FEB-2024
DEFINITION  3 stopcodons in 3 different frames.
ACCESSION   p00044
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C730                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG925                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="E"
     misc            14..26
                     /created_by="psbuser"
                     /modified_by="psbuser"
                     /label="triple stopcodon"
     terminator      27..359
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tBdUbi1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BdUbi1T
                     (<a href=""urn:local:lapau:1x-e60q7hc"">tBdUbi1</a>)"
                     /label="BdUbi1T"
     GG              complement(360..370)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29z-ciz79d7"">F</a>"
                     /label="F"
     primer          complement(390..410)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        890..994
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    995..1855
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2026..2614
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2978..2999
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg ctctgagcta agctaggctt ctgccgaact ggttcacagt ctgctgccct
       61 tggtggtctg ccccttagtg gtcatgcctt ttgttatgtg tcttgcgtcc caatcctgta
      121 tcgtttgtgt gaacatctct gctgctgtat agcagcttga atcctgttat gaatttgtga
      181 acctgaacct tgttccgtga atcatgttat gaataagtga acctgaacct tgttccgtga
      241 ttattgttac aatctgttgt gccgtatggt tggtcgtgtg tgatttatgt tgaactggag
      301 aaccaagttc gttccaggac atattgcaac ctaagctaaa ccatgtagaa ctacttgtta
      361 ctatgagacc gaattctcgc cctatagtga gtcgtattac ctggccgtcg ttttacaacg
      421 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      481 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      541 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      601 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      661 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      721 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      781 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1681 tgctgataaa tctggagccg gtgagcgtgg ctctcgaggt atcattgcag cactggggcc
     1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2761 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2821 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2881 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2941 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     3001 gagctattta ggtgacacta tagaagtgaa gctt
//
