LOCUS       pGG-C-ZmGRF1-ZmGIF1-D        4641 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.yl-h3vgkl1
VERSION     urn.local.psbuser.yl-h3vgkl1
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##Clone_number-START##                                             
            Clone number :: C616                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG769                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..1966
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmGRF1-linker-ZmGIF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ZmGRF-ZmGIF (<a
                     href=""urn:local:lapau:1y-dttkko1"">ZmGRF1-linker-ZmGIF1</
                     a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/0;
                     ZmGRF-ZmGIF (<a
                     href=""urn:local:lapau:9u-dc98np8"">ZmGRF-linker-ZmGIF</a>
                     )"
                     /label="ZmGRF-ZmGIF"
     GG              complement(1967..1977)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /label="D"
     primer          complement(1997..2017)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2497..2601
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2602..3462
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3633..4221
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4585..4606
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggcga tgccgtatgc ctctctttcc ccggcaggcg ccgccgacca
       61 ccgctcctcc acagccacgg cgtccctcgt ccccttctgc cgctccactc cgctctccgc
      121 gggcggcggg ctgggcgagg aggacgccca ggcgagcgcg aggtggccgg ccgcgaggcc
      181 ggtggtgccg ttcacgccgg cgcagtacca ggagctggag cagcaggcgc tcatatacaa
      241 gtacctggtg gcgggcgtgc ccgttccgcc ggatctcgtg gttccaatcc gccgcggcct
      301 cgactccctc gcaacccgct tctacggcca acccacactc gggtacggac cgtacctggg
      361 gaggaaactg gatccggagc ccggccggtg ccggcgaacg gacggcaaga agtggcggtg
      421 ctccaaggag gccgccccgg actccaagta ctgcgagcgc cacatgcacc gcggccgcaa
      481 ccgttcaaga aagcctgtgg aaacgcagct cgcgccccag tcccaaccgc ccgccgccgc
      541 agccgtctcc gccgctccgc ccctggcagc cgccgccgcc gccaccacca acggcagcgg
      601 cttccagaac cactctctct acccggccat cgccggcagc actggtggtg gaggaggagt
      661 tggcgggtcc ggcaatatct cctccccgtt ctcctcgtcg atggggggat cgtctcagct
      721 gcacatggac agtgctgcca gctactccta cgcagctctt ggtggtggaa ctgcaaagga
      781 tctcaggtac aacgcttacg gaataagatc tctggcggac gagcacaacc agctgatcgc
      841 agaagccatc gactcgtcga tagagagcca gtggcgcctc cccagctcgt cgttcccgct
      901 ctcgagctac ccacatctcg gggcgctggg cgacctgggc ggccagaaca gcacggtgag
      961 ctcgctgccg aagatggaga agcagcagcc gccctcgtcc ttcctaggga acgacaccgg
     1021 ggccggcatg gccatgggct ccgcctccgc gaagcaggag ggccagacgc tgcggcactt
     1081 cttcgacgag tggcccaagg cgcgggactc ctggccgggc ctctccgacg agacggccag
     1141 cctcgcctcg ttccccccgg cgacccagct gtcgatgtcc atacccatgg cgtcctccga
     1201 cttctccgtg gccagctccc agtcgcccaa cggtgagtcg cgtacgttcc tgctggccac
     1261 ggaccgaagg gcggccgctg ccatgcagca gcaacacctg atgcagatga accagaacat
     1321 gatggggggc tacacctctc ctgccgccgt gaccaccgat ctcatccagc agtacctgga
     1381 cgagaacaag cagctgatcc tggccatcct cgacaaccag aacaatggca aggcggagga
     1441 gtgcgaacgg caccaagcta agctccagca caacctcatg tacctggccg ccatcgctga
     1501 cagccagccg ccacagaccg cgccactatc acagtacccg tccaacctga tgatgcagcc
     1561 gggccctcgg tacatgccac cgcagtccgg gcagatgatg aacccgcagt cgctgatggc
     1621 ggcgcggtcc tccatgatgt acgcgcaccc gtccctgtcg ccactccagc agcagcaggc
     1681 ggcgcacgga cagctgggta tggctccagg gggcggcggt ggcggcacga ccagcgggtt
     1741 cagcatcctc cacggcgagg ccagcatggg cggtggtggt gctggcgcag gcgccggcaa
     1801 caacatgatg aacgccggca tgttctcggg ctttggccgc agcggcagtg gcgccaagga
     1861 agggtcgacc tctctgtcgg ttgacgtccg gggtggaacc agctccggcg cgcagagcgg
     1921 ggacggcgag tacctcaaag tcggcaccga ggaagaaggc agttagtcag tgagaccgaa
     1981 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     2041 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2101 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2161 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     2221 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2281 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2341 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2401 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2461 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2521 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2581 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2641 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2701 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2761 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2821 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2881 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2941 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3001 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3061 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3121 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3181 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3241 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3301 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     3361 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3421 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4321 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4381 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4441 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4501 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4561 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     4621 gacactatag aagtgaagct t
//
