LOCUS       pGG-C-TaGRF4-TaGIF1-D        4599 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   KF718966
VERSION     KF718966.1
KEYWORDS    .
SOURCE      Cloning vector pGGC000 (unknown)
  ORGANISM  Cloning vector pGGC000
            other sequences; artificial sequences; vectors.
COMMENT     ##Clone_number-START##                                             
            Clone number :: C480                                               
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: C547                                      
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..1924
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaGRF4-linker-TaGIF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:c-cb8s5q4"">TaGRF4-linker-
                     TaGIF1</a>"
                     /label="TaGRF4-linker-TaGIF1"
     GG              complement(1925..1935)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(1955..1975)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2455..2559
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2560..3420
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3591..4179
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4543..4564
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggcga tgccgtatgc ctctctttcc ccggcaggcg accgccgctc
       61 ctccccggcc gccaccgcca ccgcctccct cctccccttc tgccgctcct cccccttctc
      121 cgccggcggc aatggcggca tgggggagga ggcgcggatg gacgggaggt ggatggcgag
      181 gccggtgccc ttcacggcgg cgcagtacga ggagctggag caccaggcgc tcatatacaa
      241 gtacctggtg gccggcgtgt ccgtcccgcc ggatctcgtg ctccccatcc gccgcggcat
      301 cgagtccctc gccgcccgct tctaccacaa ccccctcgcc atcgggtacg gatcgtacct
      361 gggcaagaag gtggatccgg agccgggccg gtgccggcgc acggacggca agaagtggcg
      421 gtgcgccaag gaggccgcct ccgactccaa gtattgcgag cgccacatgc accgcggccg
      481 caaccgttca agaaagcctg tggaaacgca gctcgtctcg cactcccagc cgccggccgc
      541 ctccgtcgtg ccgcccctcg ccaccggctt ccacaaccac tccctctacc ccgccatcgg
      601 cggcaccaac ggtggtggag gcggggggaa caacggcatg tccatgcccg gcacgttctc
      661 ctccgcgctg gggccgcctc agcagcacat gggcaacaat gccgcctctc cctacgcggc
      721 tctcggcggc gccggaacat gcaaagattt caggtatacc gcatatggaa taagatcttt
      781 ggcagacgag cagagtcagc tcatgacaga agccatgaac acctccgtgg agaacccatg
      841 gcgcctgccg ccatcttctc aaacgactac attcccgctc tcaagctact ctcctcagct
      901 tggagcaacg agtgacctgg gtcagaacaa cagcagcaac aacaacagcg gcgtcaaggc
      961 cgagggacag cagcagcagc agccgctctc cttcccgggg tgcggcgact tcggcagcgg
     1021 cgactccgcg aagcaggaga accagacgct gcggccgttc ttcgacgagt ggccgaagac
     1081 gagggactcg tggtcggacc tgaccgacga caactcgaac gtcgcctcct tctcggccac
     1141 ccagctgtcg atctcgatac ccatgacgtc ctccgacttc tccgccgcca gctcccagtc
     1201 gcccaacggc atgctgttcg ccggcgaaat gtacgcggcc gctgccatgc agcagcaaca
     1261 cctgatgcag atgaaccaga gcatgatggg gggctacgct tcctctacca ctgccaccac
     1321 tgatctcatt cagcagtacc tggatgagaa caagcagctg atcctggcca tcctcgacaa
     1381 ccagaacaac ggcaaggtgg aggagtgcgc acggaaccaa gctaagctcc agcagaacct
     1441 catgtacctc gccgccatcg ccgacagcca gcctccgcag acggcatcgc tgtctcagta
     1501 cccgtccaac ctgatgatgc agtccgggcc gcggtacatg cagcagcagt cggcgcagat
     1561 gatgtcgccg cagtcgctga tggcggcgcg gtcgtcgatg atgtacgcgc agcaggccat
     1621 gtcgccgctc cagcagcagc agcagcagca gcagcaccag gcggccgcgc acggccagct
     1681 ggggatgtcc tccggcgcga ccaccgggtt caacctcctg cacggtgagg ccagcatggg
     1741 cggcggcggc ggcgccactg gcaacagcat gatgaacgcc agcgtcttct cggactatgg
     1801 ccgcggcggc agcggcgcca aggaggggtc gacctcgctg tcggccgacg ctcgcggcgc
     1861 caactctggc gcgcacagcg gcgacgggga gtacctcaag ggcaccgagg aggaaggaag
     1921 ctagtcagtg agaccgaatt ctcgccctat agtgagtcgt attacctggc cgtcgtttta
     1981 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     2041 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     2101 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     2161 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2221 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2281 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2341 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2401 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2461 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2521 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2581 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2641 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2701 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2761 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2821 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     2881 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     2941 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3001 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3061 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3121 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3181 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3241 tttattgctg ataaatctgg agccggtgag cgtggctctc gaggtatcat tgcagcactg
     3301 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3361 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3421 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3481 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3541 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3601 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3661 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3721 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3781 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3841 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3901 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3961 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4021 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4081 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4141 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4201 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4261 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4321 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     4381 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     4441 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     4501 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     4561 cacacgagct atttaggtga cactatagaa gtgaagctt
//
