LOCUS       pGG-A-pOsU3-sgRNA(ZmCHAOS)-B        3227 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local...wh-a93k265
VERSION     urn.local...wh-a93k265
KEYWORDS    .
SOURCE      urn.local...g-7zj7gen
  ORGANISM  urn.local...g-7zj7gen.
COMMENT     ##Clone_number-START##                                             
            Clone number :: C71                                                
            ##Clone_number-END##                                               
            ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG37                                      
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..449
                     /modified_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OsU3"
                     /Transferred_Similarity="98.67%"
                     /Primary_Match="98.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:71-dgycryx"">OsU3</a>"
                     /label="OsU3"
     spacer          450..469
                     /created_by="psbuser"
                     /label="ZmCHAOS spacer"
     Scaffold        470..552
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Scaffold(<a
                     href=""urn:local:thjac:2f-c4nnd55"">SpCas9Scaffold</a>)"
     GG              complement(553..563)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:28v-ciz6xqo"">B</a>"
                     /label="B"
     primer          complement(583..603)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1083..1187
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1188..2048
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2219..2807
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3171..3192
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tagtaattca tccaggtcac caagttctag gattttcaga actgcaactt
       61 attttatcaa ggaatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact
      121 taaagttatc aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc
      181 acaagacagg cgtctactac tggtgctacc agcaaatgct ggaagccggg aacactgggt
      241 acgttggaaa ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta
      301 gtgggccatg aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg
      361 gacgacaaca aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt
      421 taaaagagtt gtgcagatga tccgtggcac tggtcctgga acaccgcggg ttttagagct
      481 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      541 ggtgcttttt ttaacatgag accgaattct cgccctatag tgagtcgtat tacctggccg
      601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      721 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
      781 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
      841 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      961 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     1021 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     1081 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1141 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1201 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1261 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1321 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1381 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1441 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1501 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1561 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1621 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1681 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1741 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1801 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1861 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga ggtatcattg
     1921 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1981 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2041 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2101 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2161 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2221 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2281 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2341 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2401 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2461 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2521 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2581 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2641 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2701 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2761 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2821 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2881 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2941 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3001 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3061 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3181 aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
