LOCUS       pGG-A-pOsAct1-C         4101 bp    DNA     circular UNA 05-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.su-h3vcd58
VERSION     urn.local.psbuser.su-h3vcd58
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     ##E.coli_Glycerol_Stock-START##                                    
            E.coli Glycerol Stock :: CG938                                     
            ##E.coli_Glycerol_Stock-END##
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1426
                     /note="Unknown feature type:Promoter"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=21"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pOsAct"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6b-dc93wtv"">pOsAct</a>"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pOsAct"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:5u-elqmfkh"">pOsAct</a>"
                     /label="pOsAct"
     GG              complement(1427..1437)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(1457..1477)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1957..2061
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2062..2922
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3093..3681
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4045..4066
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tagctagcat actcgaggtc attcatatgc ttgagaagag agtcgggata
       61 gtccaaaata aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt
      121 ggtataaagt aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac
      181 tattataaaa attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg
      241 gtttttaagt ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc
      301 gtttgctttt gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg
      361 ctaatttgac ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat
      421 aataagatta aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata
      481 aaagataaac ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa
      541 agtgctatcc acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag
      601 tccagccaac tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg
      661 caccgcacgt ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca
      721 gcaggtgggt ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag
      781 gccggccctc cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc
      841 cctctcctcc catcccccca accctaccac caccaccacc accacctcca cctcctcccc
      901 cctcgctgcc ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac
      961 caccccgccc ctctcctctt tctttctccg tttttttttt ccgtctcgga cacgatcttt
     1021 ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg
     1081 aggggcggga tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg
     1141 gaatggggct ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt
     1201 ttaaaatttc cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat
     1261 atttttatat atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt
     1321 tttgtgggta gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt
     1381 tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta gaagccggct tgagaccgaa
     1441 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     1501 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     1561 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     1621 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1681 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1741 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1801 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1861 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1921 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2221 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2281 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2761 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     2821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3721 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3781 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3841 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3901 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3961 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4021 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     4081 gacactatag aagtgaagct t
//
