LOCUS       pG3HI-pZmUBI.2-ZmGRF1-GIF1-tNOS       11010 bp    DNA     circular UNA 16-FEB-2024
DEFINITION  .
ACCESSION   urn.local.psbuser.9m-h3v5t4a
VERSION     urn.local.psbuser.9m-h3v5t4a
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        354..381
                     /invitrogen=2250000
                     /vntifkey=29
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pDEST32-GG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Pc
                     promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pc (<a
                     href=""urn:local:.:2d-f0u4295"">GmR</a>)"
                     /label="pc"
     misc_feature    570..1103
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GmR"
                     /Transferred_Similarity="99.81%"
                     /Primary_Match="99.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2q-c4nnd56"">GmR</a>"
                     /label="GmR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1454..2042
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     rep_origin      2157..2766
                     /created_by="jonas"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="oriV"
                     /Transferred_Similarity="99.84%"
                     /Primary_Match="99.84%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:27y-hbq6m8l"">oriV</a>"
                     /label="oriV"
     regulatory      complement(2835..2858)
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 overdrive"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7j-dm4ikhd"">pTiT37 overdrive</a>"
                     /label="pTiT37 overdrive"
     regulatory      complement(2922..2946)
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7r-dm4iwd5"">pTiT37 RB</a>"
                     /label="pTiT37 RB"
     misc_feature    2958..2997
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7p-dgycryx"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     Linker          3010..3309
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker 1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:an-c544u6m"">Linker 1</a>"
                     /label="Linker 1"
     misc_feature    3320..3359
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U6"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7u-dgycryx"">U6</a>"
                     /label="U6"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        3372..5357
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:thjac:1m-c4nnd54"">pZmUBIL</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/-1008;
                     ZmUBIL.2 (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          4349..5358
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             5365..7317
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmGRF1-linker-ZmGIF1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ZmGRF-ZmGIF (<a
                     href=""urn:local:lapau:1y-dttkko1"">ZmGRF1-linker-ZmGIF1</
                     a>)"
                     /modified_by="psbuser"
                     /label="ZmGRF1-ZmGIF1"
     terminator      7322..7589
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:6c-dgycryw"">NOST</a>)"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="2 Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; tNOS (<a
                     href=""urn:local:.:fi-eaul7he"">2 Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fj-eaul7he"">3 Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:fe-eaul7he"">1 Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2u-f1y9293"">tNOS v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:13/-2;
                     tNOS (<a href=""urn:local:.:38-f1t9off"">tNOS v1</a>)"
                     /label="tNOS"
     misc_feature    7600..7639
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7v-dgycryx"">U7</a>"
                     /label="U7"
                     /note="Geneious type: Golden Gibson Cloning site"
     Linker          7652..7951
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker 7"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:b3-c545tfm"">Linker 7</a>"
                     /label="Linker 7"
     misc_feature    7962..8001
                     /created_by="lapau"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7w-dgycryx"">U8</a>"
                     /label="U8"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        8028..9442
                     /note="Unknown feature type:Promoter"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1}
                     {} 0} width 5 offset 0"
                     /note="/ApEinfo_revcolor=pink"
                     /note="/vntifkey=21"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pOsAct"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6b-dc93wtv"">pOsAct</a>"
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pOsAct"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:5u-elqmfkh"">pOsAct</a>"
                     /modified_by="psbuser"
                     /label="pOsAct1"
     Plant_Selection 8028..10908
                     /created_by="mamee"
                     /label="Hygro"
     CDS             join(9449..9787,9978..10664)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="HYG Intron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; Hyg (<a
                     href=""urn:local:lapau:o-dm37vg8"">HYG Intron</a>)"
                     /modified_by="psbuser"
                     /label="HPT"
     intron          9788..9977
                     /note="castor bean catalase intron, modified"
                     /X-Transferred_From="Hyg(Intron)"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; cat1
                     intron (<a
                     href=""urn:local:lapau:6o-dc9431i"">Hyg(Intron)</a>)"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="HYG Intron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; cat1
                     intron (<a href=""urn:local:lapau:o-dm37vg8"">HYG
                     Intron</a>)"
                     /label="cat1 intron"
     terminator      10669..10908
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="6 35ST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; t35S (<a
                     href=""urn:local:.:i9-eaulgzq"">6 35ST</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; t35S
                     (<a href=""urn:local:.:2k-f1t986v"">t35S v1</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:14/0;
                     t35S (<a href=""urn:local:.:lk-f0ukkf4"">t35S v2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:164/-6;
                     CaMV Poly(A) signal (<a
                     href=""urn:local:.:cr-gh4a2i2"">pDe-Cas9 - Basta</a>)"
                     /label="t35S"
     misc_feature    10919..10958
                     /standard_name="U9"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7x-dgycryx"">U9</a>"
                     /note="Geneious type: Golden Gibson Cloning site"
     regulatory      complement(10986..11010)
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pTiT37 LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7n-dm4itu5"">pTiT37 LB</a>"
                     /label="pTiT37 LB"
ORIGIN      
        1 ccagctcgag gcggcgccct gcagccagcc aacagctccc cgaccggcag ctcggcacaa
       61 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
      121 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
      181 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
      241 cgttgctgcc tgtgatcacc gcggtttcaa ccttgtattc accggtgtta tggttaatta
      301 acagcttcaa ctttattata caaagttgat aattcactgg cgcgccgcta gtttgacata
      361 agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      421 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      481 ctgttttttt gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      541 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagcaacgat gttacgcagc
      601 agggcagtcg ccctaaaaca aagttaggtg gctcaagtat gggcatcatt cgcacatgta
      661 ggctcggccc tgaccaagtc aaatccatgc gggctgctct tgatcttttc ggtcgtgagt
      721 tcggagacgt agccacctac tcccaacatc agccggactc cgattacctc gggaacttgc
      781 tccgtagtaa gacattcatc gcgcttgctg ccttcgacca agaagcggtt gttggcgctc
      841 tcgcggctta cgttctgccc aagtttgagc agccgcgtag tgagatctat atctatgatc
      901 tcgcagtctc cggcgagcac cggaggcagg gcattgccac cgcgctcatc aatctcctca
      961 agcatgaggc caacgcgctt ggtgcttatg tgatctacgt gcaagcagat tacggtgacg
     1021 atcccgcagt ggctctctat acaaagttgg gcatacggga agaagtgatg cactttgata
     1081 tcgacccaag taccgccacc taatcaattc gttcaagccg agatcggctt cccggccgcg
     1141 gagttgttcg gtaaattgtc acaacgccgc gaatatagtc tttaccatgc ccttggccac
     1201 gcccctcttt aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca
     1261 taacaaaagt ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat
     1321 tctgtctagt ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt
     1381 tattgtccgc ccacacccgc ttcgccccgt tccactgagc gtcagacccc gtagaaaaga
     1441 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1501 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1561 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1621 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1681 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1741 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1801 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1861 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1921 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1981 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2041 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2101 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agatagctgg
     2161 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     2221 cgaagccgtg tgcgagacac cccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
     2281 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     2341 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     2401 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     2461 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     2521 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
     2581 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     2641 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     2701 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc cttctcgaac
     2761 cctcccggtc gagtcatggt ctagcatgga tctcggggac gtcgtacttt gatcccgagg
     2821 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     2881 tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
     2941 ctgtcaagct tgagctccat tactcgcatc cattctcagg ctgtctcgtc tcgtctcctc
     3001 gagatacctg atgtgcctgc tatgcttaaa tacatacctc gcccagtagc tttccaatat
     3061 gggaacatca attgtacatc gggccgggat aatcatgtcg tcacggaact tactgtaaga
     3121 gtaataattc aaaagagatg tcggtttgct agttcacgta aaggtgcctc gcgccacctc
     3181 taagtaagtg agccgtcgag acattatccc tgattttctc actactatta gtactcacgg
     3241 cgcaatacca ccacagcctt gtctcgccag aatgccggtc agcatatgga agacctcaag
     3301 gcaggtcaag tattctagac tcgttcgctg ccacctaaga atactctacg gtcacatacc
     3361 tcgagatacc tgtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca
     3421 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt
     3481 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta
     3541 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac
     3601 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc
     3661 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca
     3721 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc
     3781 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt
     3841 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat
     3901 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc
     3961 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga
     4021 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac
     4081 cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc
     4141 cggcacggca ggcggcctcc tcctcctctc acggcacggc agctacgggg gattcctttc
     4201 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
     4261 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     4321 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     4381 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     4441 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     4501 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     4561 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     4621 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     4681 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     4741 cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat
     4801 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     4861 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     4921 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     4981 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     5041 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     5101 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     5161 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     5221 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     5281 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     5341 ttggtgttac ttctgcaggg ctccatggcg atgccgtatg cctctctttc cccggcaggc
     5401 gccgccgacc accgctcctc cacagccacg gcgtccctcg tccccttctg ccgctccact
     5461 ccgctctccg cgggcggcgg gctgggcgag gaggacgccc aggcgagcgc gaggtggccg
     5521 gccgcgaggc cggtggtgcc gttcacgccg gcgcagtacc aggagctgga gcagcaggcg
     5581 ctcatataca agtacctggt ggcgggcgtg cccgttccgc cggatctcgt ggttccaatc
     5641 cgccgcggcc tcgactccct cgcaacccgc ttctacggcc aacccacact cgggtacgga
     5701 ccgtacctgg ggaggaaact ggatccggag cccggccggt gccggcgaac ggacggcaag
     5761 aagtggcggt gctccaagga ggccgccccg gactccaagt actgcgagcg ccacatgcac
     5821 cgcggccgca accgttcaag aaagcctgtg gaaacgcagc tcgcgcccca gtcccaaccg
     5881 cccgccgccg cagccgtctc cgccgctccg cccctggcag ccgccgccgc cgccaccacc
     5941 aacggcagcg gcttccagaa ccactctctc tacccggcca tcgccggcag cactggtggt
     6001 ggaggaggag ttggcgggtc cggcaatatc tcctccccgt tctcctcgtc gatgggggga
     6061 tcgtctcagc tgcacatgga cagtgctgcc agctactcct acgcagctct tggtggtgga
     6121 actgcaaagg atctcaggta caacgcttac ggaataagat ctctggcgga cgagcacaac
     6181 cagctgatcg cagaagccat cgactcgtcg atagagagcc agtggcgcct ccccagctcg
     6241 tcgttcccgc tctcgagcta cccacatctc ggggcgctgg gcgacctggg cggccagaac
     6301 agcacggtga gctcgctgcc gaagatggag aagcagcagc cgccctcgtc cttcctaggg
     6361 aacgacaccg gggccggcat ggccatgggc tccgcctccg cgaagcagga gggccagacg
     6421 ctgcggcact tcttcgacga gtggcccaag gcgcgggact cctggccggg cctctccgac
     6481 gagacggcca gcctcgcctc gttccccccg gcgacccagc tgtcgatgtc catacccatg
     6541 gcgtcctccg acttctccgt ggccagctcc cagtcgccca acggtgagtc gcgtacgttc
     6601 ctgctggcca cggaccgaag ggcggccgct gccatgcagc agcaacacct gatgcagatg
     6661 aaccagaaca tgatgggggg ctacacctct cctgccgccg tgaccaccga tctcatccag
     6721 cagtacctgg acgagaacaa gcagctgatc ctggccatcc tcgacaacca gaacaatggc
     6781 aaggcggagg agtgcgaacg gcaccaagct aagctccagc acaacctcat gtacctggcc
     6841 gccatcgctg acagccagcc gccacagacc gcgccactat cacagtaccc gtccaacctg
     6901 atgatgcagc cgggccctcg gtacatgcca ccgcagtccg ggcagatgat gaacccgcag
     6961 tcgctgatgg cggcgcggtc ctccatgatg tacgcgcacc cgtccctgtc gccactccag
     7021 cagcagcagg cggcgcacgg acagctgggt atggctccag ggggcggcgg tggcggcacg
     7081 accagcgggt tcagcatcct ccacggcgag gccagcatgg gcggtggtgg tgctggcgca
     7141 ggcgccggca acaacatgat gaacgccggc atgttctcgg gctttggccg cagcggcagt
     7201 ggcgccaagg aagggtcgac ctctctgtcg gttgacgtcc ggggtggaac cagctccggc
     7261 gcgcagagcg gggacggcga gtacctcaaa gtcggcaccg aggaagaagg cagttagtca
     7321 gctagagtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     7381 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     7441 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     7501 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     7561 gcgcggtgtc atctatgtta ctagatcgag tattctagac aagacgctgg ctctgacatt
     7621 tccgctactg aactactcgc tcgagatacc ttgaatggcc ggaagtggta cggacgcaat
     7681 atgcgcgggt gagagggcaa ataggcaggt tcgcctacgt tacgctaggg ggcgattcta
     7741 taagaatgca cattgcatcg atacataaga tgtctcgacc gcatgcgcaa cttgtgaagt
     7801 gtctactatc cctaagccca tttctcgcac aataacccct gaatgtgtcc gcatctgatg
     7861 ttaccggggt tgagttagtg tcgagctcgc ggaacttatt gcatgagtag agatatgtaa
     7921 gagctgttag atagctcgct gagctaatag tgtattctag acctcgtctc aaccaaagca
     7981 atcaacccat caaccacctg gctcgagata ctaggatatc ggcacctagc tagcatactc
     8041 gaggtcattc atatgcttga gaagagagtc gggatagtcc aaaataaaac aaaggtaaga
     8101 ttacctggtc aaaagtgaaa acatcagtta aaaggtggta taaagtaaaa tatcggtaat
     8161 aaaaggtggc ccaaagtgaa atttactctt ttctactatt ataaaaattg aggatgtttt
     8221 tgtcggtact ttgatacgtc atttttgtat gaattggttt ttaagtttat tcgcttttgg
     8281 aaatgcatat ctgtatttga gtcgggtttt aagttcgttt gcttttgtaa atacagaggg
     8341 atttgtataa gaaatatctt taaaaaaacc catatgctaa tttgacataa tttttgagaa
     8401 aaatatatat tcaggcgaat tctcacaatg aacaataata agattaaaat agctttcccc
     8461 cgttgcagcg catgggtatt ttttctagta aaaataaaag ataaacttag actcaaaaca
     8521 tttacaaaaa caacccctaa agttcctaaa gcccaaagtg ctatccacga tccatagcaa
     8581 gcccagccca acccaaccca acccaaccca ccccagtcca gccaactgga caatagtctc
     8641 cacacccccc cactatcacc gtgagttgtc cgcacgcacc gcacgtctcg cagccaaaaa
     8701 aaaaaaaaga aagaaaaaaa agaaaaagaa aaaacagcag gtgggtccgg gtcgtggggg
     8761 ccggaaacgc gaggaggatc gcgagccagc gacgaggccg gccctccctc cgcttccaaa
     8821 gaaacgcccc ccatcgccac tatatacata cccccccctc tcctcccatc cccccaaccc
     8881 taccaccacc accaccacca cctccacctc ctcccccctc gctgccggac gacgagctcc
     8941 tcccccctcc ccctccgccg ccgccgcgcc ggtaaccacc ccgcccctct cctctttctt
     9001 tctccgtttt ttttttccgt ctcggacacg atctttggcc ttggtagttt gggtgggcga
     9061 gaggcggctt cgtgcgcgcc cagatcggtg cgcgggaggg gcgggatctc gcggctgggg
     9121 ctctcgccgg cgtggatccg gcccggatct cgcggggaat ggggctctcg gatgtagatc
     9181 tgcgatccgc cgttgttggg ggagatgatg gggggtttaa aatttccgcc atgctaaaca
     9241 agatcaggaa gaggggaaaa gggcactatg gtttatattt ttatatattt ctgctgcttc
     9301 gtcaggctta gatgtgctag atctttcttt cttctttttg tgggtagaat ttgaatccct
     9361 cagcattgtt catcggtagt ttttcttttc atgatttgtg acaaatgcag cctcgtgcgg
     9421 agcttttttg taggtagaag ccggctccat gaaaaagcct gaactcaccg cgacgtctgt
     9481 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     9541 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     9601 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     9661 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     9721 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     9781 tctgcaggta aatttctagt ttttctcctt cattttcttg gttaggaccc ttttctcttt
     9841 ttattttttt gagctttgat ctttctttaa actgatctat tttttaattg attggttatg
     9901 gtgtaaatat tacatagctt taactgataa tctgattact ttatttcgtg tgtctatgat
     9961 gatgatgata actgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
    10021 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
    10081 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
    10141 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
    10201 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
    10261 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
    10321 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
    10381 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
    10441 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
    10501 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
    10561 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
    10621 gtggaaaccg acgccccagc actcgtccga gggcaaagga atagtcagga taactagctg
    10681 agcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct
    10741 atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg
    10801 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
    10861 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgt attctagagt
    10921 tccttatcat ctggcgaatc ggacccacaa gagcactggg gccctctaga ggatccccgg
    10981 gtactgttta caccacaata tatcctgcca
//
