LOCUS       pDONRP2R.P3-mScarlet-HOTag6        3569 bp ds-DNA     circular SYN 28-SEP-2023
DEFINITION  .
ACCESSION   urn.local...79-gszzt3z
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   alsaf
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2019 from SnapGene 4.2.0 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|871862400|.
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
     Linker          128..169
                     /modified_by="mamee"
                     /label="linker"
     CDS             170..865
                     /vntifkey=4
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mScarlet"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="96.67%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-24
                     (adjusted to 96.67%; Adjusted-Interval-0:0/0); mScarlet-L
                     (<a href=""urn:local:lapau:r-e4ew2m2"">mScarlet</a>)"
                     /label="mScarlet-L"
     Linker          866..952
                     /modified_by="mamee"
                     /label="Linker2"
     CDS             953..1054
                     /note="/vntifkey=21"
                     /modified_by="mamee"
                     /label="HO-Tag6"
     attL3           complement(1056..1151)
     primer          complement(1209..1229)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        1290..1340
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1341..2150
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2297..2885
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     primer          3484..3504
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtggtactc gagtccggac tcagatctgg cagcggtgga ggcgatatca tggtgagcaa
      181 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
      241 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      301 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
      361 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
      421 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      481 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
      541 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg
      601 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
      661 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
      721 caagaagccc gtgcagatgc ccggcgccta caacgtcgac cgcaagttgg acatcacctc
      781 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
      841 cggcggcatg gacgagctgt acaagggcgg actcgagtcc ggactcagat ctggcagcgg
      901 tggaggcgat atcggcggac tcgagtccgg actcagatct ggcagcggtg gaaccttgag
      961 agaaatcgag gaattgttga ggaaaatcat tgaagacagc gtgcgcagtg tggccgagct
     1021 ggaggacatt gagaagtggc tgaagaaaat ctaaacaact ttattataca aagttggcat
     1081 tataaaaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1141 aatcattatt tggagctcca tggtagcgtt aacgcggccg cgatatcccc tatagtgagt
     1201 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     1261 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     1321 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     1381 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     1441 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     1501 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     1561 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     1621 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     1681 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     1741 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     1801 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     1861 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     1921 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1981 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2041 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2101 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2161 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     2221 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     2281 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2341 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2401 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2461 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2521 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2581 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2641 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     2701 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2761 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2821 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2881 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2941 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3001 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3061 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3121 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     3181 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     3241 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     3301 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     3361 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     3421 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     3481 gttgtaaaac gacggccagt cttaagctcg ggccctgcag ctctagagct cgaattctac
     3541 aggtcactaa taccatctaa gtagttgat
//
